Analysis of biomarkers for the cross-linkage of formaldehyde with bovine serum albumin peptides

Formaldehyde, a well-known environmental toxic hazard, has been found to produce endogenously via semicarbazide-sensitive amine oxidase-catalyzed oxidative deamination of methylamine. In diabetes, the activity of SSAO has been found to increase with a subsequent increase in endogenous formaldehyde production. It has been postulated that SSAO-induced production of formaldehyde may be involved in the alteration of protein structure, which may subsequently cause protein deposition associated with chronic pathological disorders. Formaldehyde has also been found to react （cross-link） with amino group of the N-terminal amino acid residue and with the side-chains of arginine, cysteine, histidine and lysine residues. Therefore, formaldehyde may be responsible, at least in part, for protein cross-linkage, oxidative stress and cytotoxicity. The cross-linking of formaldehyde with bovine serum albumin was studied using LC-MS and Mascot database. The peptides sequence for control BSA （untreated） digested with trypsin was matched in the online database search query by exporting the MS/MS data to online MASCOT database. In this way, a total of twenty-seven peptides were matched in the database search query. These twenty-seven peptides were then searched manually in all of the tryptic BSA samples treated with different concentrations of FA that were incubated in different time intervals. Six formaldehyde-treated BSA peptides （FKDLGEEHFK, HLVDEPQNLIK, KVPQVSTPTLVEVSR, RPCFSALTPDETYVPK, LVNELTEFAK, DAFLGSFLYEYSR） were found to be the possible markers for formaldehyde-protein/peptides adducts.     

Preparation and characterization of 3-(triethoxysilyl) propyl isocyanate self-assembled monolayer on surface of chip

Monolayer of 3-(triethoxysilyl) propyl isocyanate was prepared on the slide by self-assembled tech-nique. X-ray photoelectron spectroscopy (XPS) was employed to analyze the elementary composition of the film. Contact angle of distilled water was measured to characterize the surface state. It was shown that 3-(triethoxysilyl) propyl isocyanate had been successfully assembled on the slide. The in-crease of contact angle to 80° demonstrated that the hydrophobicity of the surface of chip was in-creased signific...     

一种基于顺序图的软件性能分析方法

软件性能工程SPE是一种重要的性能分析方法，它将UML顺序图转化为执行图，进而分析软件的性能是否符合期望的指标。然而软件性能工程（SPT）却未给出顺序图到执行图的具体转化方法，在实际应用特别是自动实现时存在一定的困难，文中通过对模型图的形式化定义，提出了一种顺序图转换为执行图的基本算法，并给出了一种基于顺序图的软件性能评价方法。     

Liquid droplet as efficient master in thin membrane fabrication of poly(dimethylsiloxane) microfluidic devices

In this paper,liquid droplet master was for the first time used to fabricate thin membranes integrated polymeric microfluidic devices.As demonstration,water liquid droplet master was used to make a thin membrane in PDMS microfluidic devices which were prepared by using printed master.The PDMS membrane can be controlled down to approximately 10 μm thick with considerable vapour permeation which was adjusted by environmental humidity.An array of the microfluidic devices with thin PDMS membranes was also used ...     

多层交替沉积后退火处理MgB2超导薄膜上约瑟夫森结的制备

在多层交替(SiC/[Mg/B]5)沉积后退火处理的MgB2薄膜上用紫外光刻和Ar离子刻蚀制作出SQUID环路膜条,然后用聚焦离子束(FIB)刻蚀方法在SQUID的环路上制作了150～300nm之间不同尺寸的纳米微桥结构,并测量了其电阻温度(R-T)曲线和电流电压(I-V)曲线.膜条的R-T曲线与薄膜基本相同,表明薄膜没有受到膜条制备过程中潮湿的影响.对SQUID的R-T关系测量发现电阻有较大升高,并看到由纳米微桥的存在而具有的结构.SQUID的I-V曲线表明,纳米微桥形成了弱连接,超流主要体现为约瑟夫森耦合电流.其中一个150nm宽纳米微桥的SQUID,其回滞消失的温度约为10K,在此温度下,得到临界电流Ic约为4.5mA,IcRN～2.25mV,单个纳米微桥结的临界电流密度约为1.5×107A/cm2.临界电流Ic随温度以幂指数关系变化,也验证了纳米微桥的弱连接特性.我们的实验对基于MgB2薄膜的约瑟夫森器件制备具有参考价值.     

Semi-crosslinked polyacrylamides as high-resolution and dynamic self-coating sieving matrices for protein capillary electrophoresis

This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked polyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes.     

Generating oxygen adatoms on Au(997) by thermal decomposition of NO<Subscript>2</Subscript>

We report our investigation of the interaction of NO₂ with the Au(997) vicinal surface by high-resolution photoelectron spectroscopy using synchrotron radiation as the excitation source. At 170 K, both core-level and valence-band photoemission results illustrate the decomposition of NO₂ on the Au(997) surface at low NO₂ exposures, forming coadsorbed NO(a) and O(a) species. After annealing at 300 K, NO(a) desorbs from Au(997) whereas O(a) remains on the surface. Upon annealing at 750 K, we observe no signal for adsorbed oxygen on Au(997). These results clearly demonstrate that thermal decomposition of NO₂ is an effective method to generate oxygen adatoms on Au(997) under ultrahigh-vacuum conditions.     

GD121P卷接机组吸丝成形关键技术的CFD仿真分析

采用计算流体力学数值仿真方法,对以GD121P卷接机组为原型的吸丝成形关键技术进行了研究.给出了问题的仿真方法,建立了仿真模型,并根据实验对比建立了吸丝带多孔介质模型.仿真获得了吸丝带附近压强场关键特征,结果表明:在烟丝平整器前端,吸丝带上下压差较大,有利于形成较紧密的烟丝层,降低回丝量;仿真结果符合工程实际.     

新型HLA-A2限制的B, C型HBV特异性CTL表位的筛选及鉴定

乙型肝炎病毒(HBV)特异性CD8+ T细胞(CTL)介导的细胞免疫应答在控制HBV感染和病毒清除中至关重要, 已鉴定的T细胞表位绝大多数都来自A, D基因型的乙肝病毒, 而在我国流行的B, C型病毒表位研究较少. 本研究合成重叠九肽肽库, 通过细胞结合试验和体外重折叠实验系统筛选和鉴定B型和C型HBV核心蛋白表位, 发现一个在60位氨基酸由L变异成V(L60V)而产生的新表位HBcAg60-68. 表位肽能在HLA-A2/K^b转基因小鼠体内激发特异性CTL细胞免疫反应. 检测HLA-A2阳性乙肝患者表位特异性CTL证明该九肽序列为体内自然加工的表位, 这为研究HBV感染中特异性CTL免疫应答提供新的依据.