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研究了疏水柱吸附分离工业酵母(S.Cerevisiae)中的L-天门冬氨酸酶.考察了三种疏水吸附剂TskgelButy_Toyopearl,TskgelPhenyl_Toyopearl和TsKgelEther_Toyopearl650C的吸附性能.研究表明,通过选择控制流动相的pH值和离子强度,一次吸附过程中L-天门冬氨酸酶的分离纯化纯度可提高14倍并保持较高的活性。实验还考察了吸附等温线类型和温度等因素的影响.结果表明,酵母中分子的电荷和疏水性是疏水柱吸附的重要影响因素. 相似文献
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Onouchi Y Gunji T Burns JC Shimizu C Newburger JW Yashiro M Nakamura Y Yanagawa H Wakui K Fukushima Y Kishi F Hamamoto K Terai M Sato Y Ouchi K Saji T Nariai A Kaburagi Y Yoshikawa T Suzuki K Tanaka T Nagai T Cho H Fujino A Sekine A Nakamichi R Tsunoda T Kawasaki T Nakamura Y Hata A 《Nature genetics》2008,40(1):35-42
Kawasaki disease is a pediatric systemic vasculitis of unknown etiology for which a genetic influence is suspected. We identified a functional SNP (itpkc_3) in the inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) gene on chromosome 19q13.2 that is significantly associated with Kawasaki disease susceptibility and also with an increased risk of coronary artery lesions in both Japanese and US children. Transfection experiments showed that the C allele of itpkc_3 reduces splicing efficiency of the ITPKC mRNA. ITPKC acts as a negative regulator of T-cell activation through the Ca2+/NFAT signaling pathway, and the C allele may contribute to immune hyper-reactivity in Kawasaki disease. This finding provides new insights into the mechanisms of immune activation in Kawasaki disease and emphasizes the importance of activated T cells in the pathogenesis of this vasculitis. 相似文献
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T. Yoshikawa Y. Furukawa Y. Wakamatsu M. Kondo 《Cellular and molecular life sciences : CMLS》1982,38(4):501-502
Summary Immunopotentiators such as BCG, levamisole, PS-K and OK-432 prevent carbon tetrachloride (CCl4) hepatotoxicity, and in spite of exposure to CCl4 the liver tissue levels of thiobarbituric acid (TBA) reactive substances were not increased in rats pretreated with such immunopotentiators. 相似文献
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Shanghai and Yokohama are very similar in the positions in their respective state economy and in the orbit of eco-nomic structure development. Since industrial districts in Yokohama city were established dozens years earlier than Shanghai, the experience of Yokohama must be very useful for Shanghai urban planning. 相似文献
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In nervous systems with symmetry about the midline, many neurons project axons from one side to the other. Although several of the components controlling midline crossing have been identified, little is known about how axons choose the appropriate pathway when crossing. For example, in the Drosophila embryo axons cross the midline in one of two distinct tracts, the anterior or posterior commissure (AC or PC, respec tively). Here we show that the Derailed (Drl) receptor tyrosine kinase is expressed by neurons that project in the AC, and that in the absence of Drl such neurons often project abnormally into the PC. Conversely, misexpression of Drl in PC neurons forces them to cross in the AC. The behaviour of Drl-misexpressing neurons and the in vivo binding pattern of a soluble Drl receptor probe indicate that Drl acts as a guidance receptor for a repellent ligand present in the PC. Our results show that Drl is a novel component in the control of midline crossing. 相似文献
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In nervous systems with bilateral symmetry, many neurons project axons across the midline to the opposite side. In each segment of the Drosophila embryonic nervous system, axons that display this projection pattern choose one of two distinct tracts: the anterior or posterior commissure. Commissure choice is controlled by Derailed, an atypical receptor tyrosine kinase expressed on axons projecting in the anterior commissure. Here we show that Derailed keeps these axons out of the posterior commissure by acting as a receptor for Wnt5, a member of the Wnt family of secreted signalling molecules. Our results reveal an unexpected role in axon guidance for a Wnt family member, and show that the Derailed receptor is an essential component of Wnt signalling in these guidance events. 相似文献
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Onouchi Y Ozaki K Burns JC Shimizu C Terai M Hamada H Honda T Suzuki H Suenaga T Takeuchi T Yoshikawa N Suzuki Y Yasukawa K Ebata R Higashi K Saji T Kemmotsu Y Takatsuki S Ouchi K Kishi F Yoshikawa T Nagai T Hamamoto K Sato Y Honda A Kobayashi H Sato J Shibuta S Miyawaki M Oishi K Yamaga H Aoyagi N Iwahashi S Miyashita R Murata Y Sasago K Takahashi A Kamatani N Kubo M Tsunoda T Hata A Nakamura Y Tanaka T;Japan Kawasaki Disease Genome Consortium;US Kawasaki Disease Genetics Consortium 《Nature genetics》2012,44(5):517-521
We performed a genome-wide association study (GWAS) of Kawasaki disease in Japanese subjects using data from 428 individuals with Kawasaki disease (cases) and 3,379 controls genotyped at 473,803 SNPs. We validated the association results in two independent replication panels totaling 754 cases and 947 controls. We observed significant associations in the FAM167A-BLK region at 8p22-23 (rs2254546, P = 8.2 × 10(-21)), in the human leukocyte antigen (HLA) region at 6p21.3 (rs2857151, P = 4.6 × 10(-11)) and in the CD40 region at 20q13 (rs4813003, P = 4.8 × 10(-8)). We also replicated the association of a functional SNP of FCGR2A (rs1801274, P = 1.6 × 10(-6)) identified in a recently reported GWAS of Kawasaki disease. Our findings provide new insights into the pathogenesis and pathophysiology of Kawasaki disease. 相似文献
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S. Baba Y. Arimoto D. Yoshikawa Y. Toyoda I. Miwa J. Okuda 《Cellular and molecular life sciences : CMLS》1979,35(8):1094-1097
Summary The localization of mutarotase in rat kidney was investigated by fluorescein-labelled and peroxidase-labelled antibody techniques, and by method of isolation of the nuclei and cytoplasm in non-aqueous solvents. In these immunohistochemical studies, mutarotase was almost exclusively recognized in the nuclei of epithelial cells of renal tubules and glomeruli in rat. The specific activity of mutarotase was found to be 1.5 times higher in the nuclei (122 units/g dry wt) than that in the cytoplasm (80 units/g dry wt) isolated with non-aqueous solvents. These results suggest that mutarotase may be involved in the metabolism of D-glucose in nuclei.Acknowledgment. The authors thank Prof. Y. Nishizuka (Department of Biochemistry, School of Medicine, Kobe University) for his valuable advice. 相似文献