Investigation of hrDNA targeting vector-mediated tumor-specific suicide gene therapy for hepatocellular carcinoma

Vectors pose most pivotal problem of gene therapy[1]. Because of the high transfection efficiency both in vitro and in vivo, the viral vector has been employed in 70% clinical trials of gene therapy (http://www.wiley.co.uk/ genmed/clinical). However, thei…     

Silica nanoparticle is a possible safe carrier for gene therapy

In order to develop a safe and effective gene therapy carrier, some toxicological and biodynamical experiments were carried out on silica nanoparticles （SiNPs）. First we prepared SiNPs with appropriate portions of cyclohexane, deionized water and ethyl silicate, and then transfected the modified SiNPs and GFP plasmid DNA complex into the HT1080 cells to test the effectiveness of transfection for gene therapy. At the same time, we injected the SiNPs into a number of mice through tail vein. Then we made the mice crossed to evaluate the acute, long-term and reproductive toxicity. In vivo distribution analysis and pathological examination were made on both adult mice and their offspring. SiNPs were uniform and had an average diameter of 40 nm, and the modified SiNPs carried exogenous DNA molecules into target cells and the transferred GFP fusion gene was effectively expressed in the cells. The SiNPs injected via tail vein were widely distributed in almost all of tissues, and the injected mice had the ability to reproduce normally. The in vivo and in vitro results of this study clearly show that SiNPs can be used as a safe and effective carrier for gene transfection and gene therapy.     

树突状细胞抗肿瘤疫苗的基础及临床研究现状

树突状细胞（Dendritic Cells,DCs）是目前发现的免疫系统中功能最强的专职递呈细胞。大量研究表明DCs疫苗在抗肿瘤免疫性中扮演着非常重要的角色。本文就DC的生物学特性及功能？在临床肿瘤治疗中的意义？国内外应用DC治疗常见肿瘤的现状进行综述。这将为有关DC的基础和临床试验提供有价值的信息。     

Expression of reconstructive hFⅧ in the hrDNA by using hrDNA targeting vector

Our lab has constructed a new nonviral vector-hrDNA targeting vector（pHrneo）, pHrneo is a human derived vector that can target gene into human ribosomal DNA（hrDNA） locus. In this study, we inserted expression cassette of reconstructive hF Ⅷ （hFⅧ-BDDAK39） to pHrneo to construct targeting vector： pHrneo-BDDAK39. Through electroporation of pHrneo-BDDAK39 into HT1080 cells, we identified the homologous recombinants by PCR and Southen blotting, and tested the expression of hFⅧ- BDDAK39 in the hrDNA locus. The hFⅧ-BDDAK39 was successfully targeted into hrDNA locus of HT-1080 by pHrneo-BDDAK39, and the efficiency of site-specific integration was 2.0×10^-5. hFⅧ-BDDAK39 in hrDNA locus of HT-1080 is found to be able to express efficiently （32±5 ng·10^6 cells^-1 .24 h^-1）. Targeting vector pHrneo-BDDAK39 can find use in gene therapy for hemophilia.     

软弱地层深大基坑富水特性及组合支护控制

为研究深大基坑地层富水特性以及组合支护方案下基坑控制效果,以在建连云港—镇江高速铁路线淮安东站站前广场基坑为工程背景,开展含水层抽水试验,明确地层富水特性以及水力联系,为基坑施工提供依据;设计地连墙、混合搅拌壁式地下连续墙(trench-cutting&re-mixing deep wall, TRD)两种支护以及分层开挖方案,并开展了基坑控制效果现场监测分析。结果表明：(1)影响含水层渗透性因素较多,应采用多种方法比对分析,获取较为合理的水文参数;本工程含水层潜水静水位标高+6.60～+6.80 m,第Ⅰ承压含水层静水位标高+3.73～+3.75 m,潜水和承压水之间有较厚的弱透水隔水层,两者之间没有明显的水力联系。(2)地连墙和TRD连接处深层水平位移最大,需要加强重点防控;地连墙侧位移分布近似均匀,TRD墙侧呈现上、中部大,下部小的“刀把”形,地连墙侧水平位移以及地表沉降控制效果均优于TRD墙侧。(3)桩顶和立柱隆沉具有明显的三阶段变化特征,支护方案对桩顶和立柱隆沉的影响差异较小;混凝土撑轴力和基坑外水位变化具有明显的四阶段变化特征;支护方案对第1道混凝土支撑即次支撑轴力...