Structural analysis of the promoter of tomato 1-aminocyclopropane-1-carboxylate synthase 6 gene(Le-ACS6)

Ethylene plays an important role in the regulation of many growth and developmental processes of higher plants. In tomato,Le-ACS6,a member of the ACC synthase multigene family involved in system 1 ethylene biosynthesis during fruit ripening,is subject to negative feedback regulation by ethylene. To identify the cis-elements that are responsible for the negative feedback control,we established an in vitro transient assay system employing particle bombardment on mature-green tomato fruit pericarp to examine the expression of a luciferase(LUC) reporter gene driven by a 5′-serially deleted Le-ACS6 promoter. The results localized putative cis-elements required for negative ethylene-response between -347 and -266 upstream from the translational start site ATG. Several lines of stable transformation of the Le-ACS6 promoter and GUS reporter fusion gene containing internal deletion from -347 to -266 were generated. The expression pattern of the GUS reporter showed that removal of the nucleotides from -347 to -266 completely eliminated the response of the Le-ACS6 promoter to exogenous ethylene.     

From deep basin gas to diagenetic trap—An example from the Yulin Gas Field in the Ordos Basin

In the updip portion of the Yulin Gas Field in the Ordos Basin, there are no any structural, stratigraphic and sedimentary lithologic seals. Using thin-section petrography, ultraviolet fluorescence microscopy, micro-thermometry and Raman microspectrometry, this paper finds out the diagenetic trap and studies the diagenetic history of this field. It was revealed that three phases of diagenesis and hydrocarbon charging happened in late Triassic, late Jurassic and the end of early Cretaceous respectively. In the first two phases, acid geofluid entered the reservoir and caused dissolution and cementation. Although the porosities had been increased, further compaction accompanying re-subsidence resulted in tight sandstone and conglomerates. Till the end of the early Cretaceous, bulk of gas migrated into the tight reservoir. Cementation, however, kept on in the updip portion of this field due to low gas saturation and formed the diagenetic trap. The mechanism for gas accumulation was changed from deep basin gas to diagenetic trap, which offers a sealing condition that can retain gas for much longer time.     

Assessment of the uncertainties in temperature change in China during the last century

We have used the China Homogenized Historic Temperature dataset and some long-term station series of the neighbor countries from CRUTEM3, a 5°×5° gridded dataset of monthly mean temperature since 1900, to provide a 107-year record of surface tem-perature trends and variability. We derived a comprehensive set of uncertainty estimates to accompany the data: measurement and sampling errors, uncertainties in temperature bias estimates, and uncertainties arising from limited observational coverage on large-scale averages have all been estimated. We reanalysed the temperature changes during the period of record. The best estimates of trends for 1900–2006 with uncertainties at 95% confidence range are about 0.09±0.017°C/decade for the year as a whole, and 0.14±0.021°C/decade, 0.11±0.021°C/decade, 0.04±0.017°C/decade, and 0.07±0.017°C/decade for winter, spring, summer and autumn respectively. For 1954–2006, the trends for annual, winter, spring, summer and autumn are: 0.26±0.032°C/decade, 0.35±0.046°C/decade, 0.25±0.051°C/decade, 0.16±0.037°C/decade and 0.22±0.055°C/decade. Winter saw the most significant warming trend in both 1900–2006 and 1954–2006, while during the most recent period (the satellite era, 1979–2006), all the seasons show similar warming trends: 0.45±0.13°C/decade, 0.51±0.11°C/decade, 0.52±0.16°C/decade, 0.37±0.10°C/decade and 0.50±0.16°C/decade for annual, winter, spring, summer and autumn. Trends arising from urbanization have been evaluated as less than 5% of the total warming trend for 1951–2001, so this bias was not removed.     

Effects of silty sand on CO<Subscript>2</Subscript> corrosion behavior of low-Cr tubing steel

An experimental study was carried out to assess the effects of silty sand on the CO 2 corrosion behavior of 1 wt% Cr (1Cr) and 3 wt% Cr (3Cr) tubing steel under 0.5 MPa CO 2 at 100°C and 1.5 m/s flow velocity.The 1Cr and 3Cr specimens both suffered general corrosion,but the surface was coarser in the pure CO 2 corrosion environment.Under silty sand conditions,severe pitting corrosion occurred on the 1Cr specimens and some acicular pitting appeared on the 3Cr specimens.The average corrosion rates of 1Cr and 3Cr steels increased by factors of 3 and 1.6,respectively.The corrosion products were analyzed by scanning electron microscopy (SEM),energy dispersive spectroscopy (EDS),X-ray diffraction (XRD),and electrical impedance spectroscopy (EIS).The results show that silty sand acts as an inclusion in corrosion product films and reduces the homogeneity and density of the products,rather than abrading the corrosion film.Ion-diffusion channels may build up around the irregular silty sand;this would degrade the protective capabilities of the product films and aggravate corrosion.     

Structural analysis of the promoter of tomato 1-aminocyclopropane-l-carboxylate synthase 6 gene （Le-ACS6）

Ethylene plays an important role in the regulation of many growth and developmental processes of higher plants. In tomato, Le-ACS6, a member of the ACC synthase multigene family involved in system 1 ethylene biosynthesis during fruit ripening, is subject to negative feedback regulation by ethylene. To identify the cis-elements that are responsible for the negative feedback control, we established an in vitro transient assay system employing particle bombardment on mature-green tomato fruit pericarp to examine the expression of a luciferase （LUC） reporter gene driven by a 5＇-serially deleted Le-ACS6 promoter. The results localized putative cis-elements required for negative ethylene-response between -347 and -266 upstream from the translational start site ATG Several lines of stable transformation of the Le-ACS6 promoter and GUS reporter fusion gene containing internal deletion from -347 to -266 were generated. The expression pattern of the GUS reporter showed that removal of the nucleotides from -347 to -266 completely eliminated the response of the Le-ACS6 promoter to exogenous ethylene.     

Catalysis of organic pollutant photodegradation by metal phthalocyanines immobilized on TiO<Subscript>2</Subscript>@SiO<Subscript>2</Subscript>

A TiO2@SiO2 hybrid support was prepared by the sol-precipitation method using n-octylamine as a template.The photocatalyst manganese phthalocyanine tetrasulfonic acid (MnPcS) was immobilized on the support to form MnPcS-TiO2@SiO2.X-ray diffraction (XRD) and UV-Visible diffuse reflectance spectra (UV-Vis DRS) were employed to characterize the catalyst.The photocatalytic degradation of rhodamine B (RhB) and the catalytic oxidation of o-phenylenediamine (OPDA) under visible light irradiation were used as probe reactions.The mineralization efficiency and the degradation mechanism were evaluated using chemical oxygen demand (COD Cr) assays and electron spin resonance (ESR),respectively.RhB was efficiently degraded by immobilized MnPcS-TiO2@SiO2 under visible light irradiation.Complete decolorization of RhB occurred after 240 min of irradiation and 64.02% COD Cr removal occurred after 24 h of irradiation.ESR results indicated that the oxidation process was dominated by the hydroxyl radical (·OH) and superoxide radical (O-·2) generated in the system.     

The first determination of atmospheric phosphine in Antarctica

It is generally thought that phosphine (PH3) concentrations exist at the low ng/m3 level during the night and at the pg/m3 level during daylight in the remote atmosphere of the lower troposphere. The first de- termination of gaseous PH3 on the Antarctic Millor Peninsula is reported in this paper. No PH3 was de- tected in the air samples around 10:00 when it was sunny. However, PH3 was found in all the 10:00 air samples when it was cloudy or light snow with the average of 75.3±28.8 ng/m3 (n=5). It was also found in nearly all the samples around 22:00 with the average of 87.2±70.9 ng/m3 (n=11). Atmospheric PH3 concentrations around 22:00 were generally higher than those around 10:00 in January and they were almost the same in February. In addition, PH3 concentrations around 22:00 showed a downtrend with the decreasing air temperature, suggesting that light intensity and air temperature had an important effect on atmospheric PH3 concentration. It is very surprising to have found that high concentrations of PH3 exist in the Antarctic atmosphere under the influence of strong UV-radiation and light intensity. The tentative analyses show that dry, cold and very clean atmosphere may be very suitable for the PH3 sur- vival and cause the concentration to increase and accumulate in the local atmosphere. New ap- proaches for the PH3 formation and the process of atmospheric chemistry may exist under such an extreme environment. Atmospheric PH3 may also be from the emissions of local sources.     

金属材料的超声衰减特征及辨识的新方法

利用超声技术研究了辨识金属材料的方法.以成分相近、热处理温度相同和成分相同但热处理温度不同的多个金属材料为例,计算了金属材料对不同频率超声波的衰减系数,分析了同一频率下采样率对超声衰减谱稳定性的影响,研究了金属材料的微观组织对超声衰减的影响.结果表明,采样率越高,衰减谱的多次实验计算越趋于稳定;金属材料的超声衰减谱与其微观组织结构密切相关,其携带有金属材料的微观组织特征信息;利用金属间超声衰减谱相关系数可辨识金属材料,对贵重金属、金属文物等的防伪与辨识提供了一定的参考依据.     

关于兰州经济区产业集聚发展的思考

兰州经济区是甘肃省"十一五"规划重点发展的区域,从大地域经济组织和国家宏观发展战略上都具有重要意义。本文提出了发展九大产业集群支撑兰州经济区发展的思路以及区域整合机制。     

Responses of rice growth to copper stress under free-air CO2 enrichment （FACE）

The responses of rice to the second degree contamination of copper were studied by pot experiments under free-air CO2 enrichment （FACE） with 570 μmol ·mol^-1 of CO2. The results showed that the content of copper in rice leaves was reduced with the CO2 concentration reaching 570 μmol· mol^-1 and this happened more significantly under the second degree contamination of copper. Under FACE, activities of superoxide dismutase （SOD） enzyme in rice leaves treated by copper contamination were induced, whereas the contents of glutathione （GSH） and glutathione disulfide （GSSG） had no significant difference from the control. In the presence of ambient CO2, activities of SOD enzyme treated by copper pollution were suppressed during the whole rice growth, however, the contents of GSH and GSSG were induced at tillering and jointing stages, and then restored to the control levels in later growth under the second degree contamination of copper. With the rice growing, the content of malondialdehyde （MDA） rises continuously, but there had been no significant difference between the treatments at the same growth stage. Further studies are needed on the response mechanism of rice to Cu stress under elevated CO2.