神府烟煤和黄天棉褐煤的快速热解

用电加热金属丝网技术研究了文题。用冷阱(-196℃)捕集CO_2、CO、CH_4、C_2H_4和C-2H_6等气相组成,由气相色谱测定各气相组分析出率。考察了保温加热和脉冲加热时,挥发分析出率和各气相组成析出率随温度变化的规律。根据总包单反应模型,计算了升温过程中总挥发分析出和各气相组成析出的动力学参数。     

产结晶纤维素酶的嗜热菌鉴定及其酶学性质

从云南腾冲热海温泉采集的样品中,分离出一株产结晶纤维素酶的嗜热菌TC-1.该菌株呈杆状,革兰氏阴性,生理生化特征分析和16S rDNA序列同源性比对表明它属于亚栖热菌属Meiothermus.结晶纤维素、木聚糖和葡萄糖可以诱导该菌产生结晶纤维素酶,其中结晶纤维素诱导的酶活力最高.对其产生的结晶纤维素酶进行了初步纯化与酶学性质研究,该酶的最适反应温度为70℃,最适pH为6.0,在50℃～70℃和pH5.5～6.5之间酶活力相对稳定.     

有限二尺度方程的讨论

以 B 样条为例讨论了有限二尺度方程两方面的应用,特别从另一角度推导了 B 样条二尺度方程。     

Dating recent lake sediments using spheroidal carbonaceous particle （SCP）

Dating lake sediment using sedimentary event is the supplement and calibration to traditional dating by radionuclide such as ^210Pb and ^137Cs. Based on the change of spheroidal carbonaceous particle (SCP) concentration, the age sequence of lake sediments can be deduced. It is one of the dating methods using sedimentary event. SCP is formed from combustion of fossil fuel at high temperature up to1750℃ and at a rate of heating of approaching 104℃/s. It can be dispersed to several hundred kilometers away from its source and deposited with precipitation or dryly deposited, and kept in sediments. Compared with Cs or Pb, there is no evidence for SCP that it decays in lake sediments and is unremovable once stored except by physical disturbance because it is mainly composed of element carbon. Handy method to extract, identify and calculate has been developed. Although fossil fuel has been used early in China, combustion at high temperature started later since emergence of electricity generation. The productivity of SCP is positively related with the generated thermal power, which is reflected as the SCP concentration in lake sediments increases with the increase of generated thermal power. Therefore, reliable sediment markers from the start of the SCP record and the remarkable variation can be used for dating purpose. In China, electricity industry started from the 1950s, and rapid increase of generated power took place since 1978. Based on these time markers, SCP time sequences of lake sediment cores LH and LL-4 from Longgan Lake, the middle reach of the Yangtze River, have been established, which is comparable with the results from ^137Cs and ^210pb, and has eliminated the errors of dating using ^137Cs and ^210pb.     

双组分单分子膜行为特征的研究

以DPPC／DPPG、DPPC／DOPG、DPPC／DMPA三组二元混合物为对象,制备了相应的单分子膜;并测定其表面压-分子平均面积(π—A)曲线,运用表面热力学原理对其进行分析,建立了过量自由能与表面压、组成等参数之间的函数关系式。实验数据及计算结果表明,该关系式能定量描述双组分单分子膜在舒张-压缩循环过程中的“挤出”现象。     

植物GSTZ基因在毕赤酵母中的高效分泌表达

谷胱甘肽S-转移酶zeta类(GSTZ)是一种重要的多功能酶,与细胞生化代谢、环境净化等密切相关.将拟南芥、甘蓝型油菜"陕2B"和"垦C1"的GSTZ基因重组到表达载体pPIC9,电转化到毕赤酵母株GS115中,得到了分泌表达GSTZ酶的毕赤酵母工程菌株.甲醇诱导表达显示,最佳诱导时间在48～60 h,DCA-DC比活力为83.21～115.31 U/mg,GSTZ分泌量为25.71～42.90 U/mL.研究结果表明,植物GSTZ基因在毕赤酵母中的表达是高效的,为大规模发酵生产GSTZ奠定了基础.     

公司制对创新的推动作用与实证研究

公司制从发明到现如今的不断发展都在催生着、孕育着创新的元素。本文以实证研究的方法梳理了公司从起源到发展对社会、科学、经济中创新的启发与应用。     

嗜热厌氧产乙醇杆菌乙醇代谢途径的初步研究

以乙醇产量和细胞密度的提高为目标,从碳源和氮源入手,对嗜热厌氧产乙醇杆菌THermoanaerobacter ethanolicus JW200的培养基作了初步优化以提高乙醇代谢途径中的关键酶的得率,便于提纯.葡萄糖浓度的提高对乙醇的产生有明显的诱导作用,但当葡萄糖浓度高于2%,乙醇产量反而下降.酵母粉对乙醇产量没有促进作用,单纯提高酵母粉浓度对细胞密度无显著影响,而同时提高葡萄糖和酵母粉浓度至2.0%,OD600最高可达2.0.2%葡萄糖,0.5%酵母粉对单位细胞乙醇产量的诱导效果最佳.T.ethanolicusJW200粗酶液中未检测到丙酮酸脱羧酶的活性,经亲和柱Cibacron Blue-3GA部分纯化,在NAD洗脱蛋白中检测到辅酶A依赖型乙醛脱氢酶的活性,表明辅酶A依赖型乙醛脱氢酶是该菌乙醇代谢途径中的关键酶,它和乙醇脱氢酶共同作用,将乙酰辅酶A转化成乙醇.     

基于空间和光谱信息保持的多光谱图像融合算法

文章采用基于空间和光谱信息保持的多光谱图像融合框架,以生成具有高空间质量的多光谱图像。本文采用的融合框架的能量泛函包括四项,第一项为边缘自适应提取约束项,从全色图像中自适应提取边缘细节信息并注入多光谱图像中;第二项为线性组合系数约束项,基于对多光谱图像各波段线性组合系数的估计以改善融合图像的空间质量;第三项为光谱信息保持约束项,基于L2范数分波段估计模糊核以保持多光谱图像的光谱信息;第四项为波段比例关系保持约束项,基于融合前后波段之间的比例关系相等的假设以减轻融合图像光谱失真。在QuickBird和Pavia University图像数据上进行仿真实验,结果表明,与SFIM、MTF_GLP、MTF_GLP_HPM、PCA、GS、GSA、AIHS、GFPCA等算法相比,本文方法的融合图像具有较高的空间和光谱质量。