改革植物生理实验课教学刍议

本文就改革植物生理实验课提出自成体系开课的设想,并对改后教学计划、教学方法、成绩考核等阐述了自己的见解。     

Screening of the interacting proteins with NifA in <Emphasis Type="Italic">Azospirillum brasilense</Emphasis> Sp7 by the yeast two-hybrid system

NifA in AzospiriUum brasilense plays a key role in regulating the synthesis and activity of nitrogenase in response to ammonia and oxygen available. In this work we used the yeast two-hybrid system to identify the proteins that interact with NifA. The nifA gene was fused to the yeast two-hybrid vector pGBD-C2, and three A. brasilense Sp7 genomic libraries for use in yeast two-hybrid studies were constructed. Screening of the libraries identified four clones encoding proteins that interact with NifA. The confirmation of the interactions of each gene product of the four clones and NifA were carried out by exchanging the vectors for nifA and the four clones and by mutageneses of the four clones with shift reading frame experiments in yeast two-hybrid studies. DNA sequence analyses showed that two clones encode proteins containing PAS domains that play an important role in signal transduction. One clone has high similarity with the fhuE gene of Escherichia coli, whose gene product is involved in iron uptake and transportation, and the other clone encodes an unknown protein.     

纳米碳酸钙加入量对刚玉质浇注料性能的影响

以纯铝酸钙水泥为结合剂,研究纳米碳酸钙的加入量对刚玉质浇注料性能和显微结构的影响.结果表明,添加的纳米碳酸钙在热处理后与基质中的氧化铝反应形成片状的六铝酸钙,影响了浇注料的性能;随着纳米碳酸钙加入量的增加,在110℃热处理后浇注料的显气孔率和体积密度变化不大,耐压强度和抗折强度都略有增大,而在1000、1500、1600℃热处理后,浇注料显气孔率均逐渐增大,相应的体积密度均逐渐减小,耐压强度和抗折强度均逐渐降低.纳米碳酸钙的加入量能提高试样的强度保持率,但热震后试样的强度均明显降低;浇注料经1000～20℃一次水冷后强度保持率逐渐提高,热震性能有所改善;XRD和SEM分析结果表明,在高温热处理过程中纳米碳酸钙与刚玉反应形成片状的六铝酸钙,对结构产生不利影响,从而导致在高温热处理后,随着纳米碳酸钙加入量的增加,浇注料的显气孔率逐渐上升,强度逐渐降低.     

Interaction between PⅡ and NifA in Azospirillum brasilense Sp7

The interaction between PⅡ and NifA in A.brasilense Sp7 was investigated by using the yeast two-hybrid system.Our experimental results showed that PⅡ directly interacted with the entire NifA protein and its N-terminal domain,but did not interact with the central domin and the C-terminal domain of NifA.No interaction happened if glnB coding for PⅡ was frame-shift mutated.Pz,a homolog of PⅡ,had no interation with NifA.     

P2P系统分布式LSI的构建和更新

从P2P系统自组织和动态性特点出发,提出分布式环境下隐语义索引(LSI)构建和更新的P2P网络模型,设计适合P2P系统文档矩阵的降维表示(RDR)合并算法,结合信号和噪声子空间模型从理论上分析RDR合并算法的有效性及算法需要满足的前提条件;使用M atlab6.5针对标准文集测试RDR合并算法对查询精度的影响.理论分析和数字实验证明,该算法能够解决P2P系统中分布式LSI的构建和更新问题,能在可容忍的查询精度影响范围内,以较低的网络开销和计算量分布式地构建、更新隐语义索引.     

一种基于包丢失率保证的连接准入控制算法

介绍了一种基于等效虚拟模型的方法,对捕获的数据进行分析和参数适配,建立起基于虚拟源的等效模型和连接准入控制算法.该算法只要求用户端提供很少的流量特征参数,在包丢失率作为目标约束的条件下根据不同性质的连接源,计算用于准入控制的所有流量参数,具有对各种流量特性的可适用性.仿真表明该算法在不同程度的流突发和不同用户规模的环境下都有较好的预测性能.     

The secretion of lecithinase of Pseudomonas alcaligenes S2 was via type Ⅱ secretion pathway

Strain S2 is a lecithin （or phosphatidylcholine）- solubilizing bacterium, which was isolated from the rice rhizosphere in rural areas of Beijing, China. On the basis of a polyphasic study involving phenotypic tests, physiological and biochemical tests, 16S rDNA sequence analysis, G＋C content determination and DNA-DNA hybridizations analysis, strain S2 was identified as Pseudomonas alcaligenes. R alcaligenes S2 was mutagenized with Tn5 and four mutants showing decreased or increased solubilizing ability of lecithin were isolated based on the halo size around colonies on the solid plate supplemented with egg yolk. To characterize the genes of R alcaligenes S2 involved in solubilization of lecithin, the EcoR I fragments of the chromosomes from the four mutant strains carrying a single transposon were cloned, and the DNA sequences flanking the Tn5 were determined. The Tn5 insertion sites in the mutants M808, M1329 and M1400, showing decreased solubilizing ability of lecithin, were found to be located in the xcpS, xcpX and xcpW , respectively, whose products XcpS, XcpX and XcpW were the components of type Ⅱ secretion pathway. Complementation of xcpS, xcpX and xcpW could restore the corresponding mutants M808, M1329 and M1400 to solubilize lecithin. The data suggested that mutation in one of these xcp genes would lead to the absence of mature lecithinase secretion into the extracellular medium. The data also indicated that the secretion of lecithin-hydrolyzing enzyme of R alcaligenes was via type Ⅱ secretion pathway. In the mutant M20 showing increasing lecithin-hydrolyzing activity, the interrupted gene showed 86% identity with chpA of Pseudomonas aeruginosa PAO1, whose product plays an important role in controlling twitching motility of the bacterial ceils.     

无线多跳网络中802.11 DCF广播信道接入性能分析

分析了采用网络编码和机会路由方法的无线多跳网络中存在的信道接入问题,建模描述了802.11 DCF广播信道接入过程,并对影响无线多跳网络的吞吐率、延迟、信道资源分配等性能指标的因素进行了定量分析.利用M/M/1/K队列和离散时间的二维Markov链,建模描述具有相异报文到达速率的多个节点竞争信道时各个节点上的报文到达、排队、信道接入和发送过程.通过求解该模型,得到各节点的实际发送速率、报文平均服务时间和系统总吞吐率等指标的计算公式.将模型计算结果和基于NS-2网络模拟平台得到的实验结果进行对比.结果表明,该模型能够有效地预测各种传输负载组合情况下的报文平均服务时间和信道资源分配的公平性等网络性能指标.     

学生作息习惯与学习成绩的相关关系

以新乡学院数学与应用数学专业的41名学生为研究对象，统计了他们一学年的作息情况和学习成绩，并运用SPSS软件进行了相关性分析，得出了良好的作息习惯能有效提高学习成绩的结论。