排序方式: 共有29条查询结果,搜索用时 31 毫秒
1.
Ada Collura Laetitia Marisa Diletta Trojan Olivier Buhard Anaïs Lagrange Arnaud Saget Marianne Bombled Patricia Méchighel Mira Ayadi Martine Muleris Aurélien de Reynies Magali Svrcek Jean-François Fléjou Jean-Claude Florent Florence Mahuteau-Betzer Anne-Marie Faussat Alex Duval 《Cellular and molecular life sciences : CMLS》2013,70(4):729-742
Links between cancer and stem cells have been proposed for many years. As the cancer stem cell (CSC) theory became widely studied, new methods were developed to culture and expand cancer cells with conserved determinants of “stemness”. These cells show increased ability to grow in suspension as spheres in serum-free medium supplemented with growth factors and chemicals. The physiological relevance of this phenomenon in established cancer cell lines remains unclear. Cell lines have traditionally been used to explore tumor biology and serve as preclinical models for the screening of potential therapeutic agents. Here, we grew cell-forming spheres (CFS) from 25 established colorectal cancer cell lines. The molecular and cellular characteristics of CFS were compared to the bulk of tumor cells. CFS could be isolated from 72 % of the cell lines. Both CFS and their parental CRC cell lines were highly tumorigenic. Compared to their parental cells, they showed similar expression of putative CSC markers. The ability of CRC cells to grow as CFS was greatly enhanced by prior treatment with 5-fluorouracil. At the molecular level, CFS and parental CRC cells showed identical gene mutations and very similar genomic profiles, although microarray analysis revealed changes in CFS gene expression that were independent of DNA copy-number. We identified a CFS gene expression signature common to CFS from all CRC cell lines, which was predictive of disease relapse in CRC patients. In conclusion, CFS models derived from CRC cell lines possess interesting phenotypic features that may have clinical relevance for drug resistance and disease relapse. 相似文献
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B. S. Meldrum R. A. Vigouroux P. Rage J. B. Brierley 《Cellular and molecular life sciences : CMLS》1973,29(5):561-563
Résumé Des états de mal, durant de 3 à 7 h 30 sont provoqués par la bicuculline chez 3 babouins adolescents, immobilisés par de la gallamine et ventilés artificiellement. La tension artérielle et le débit cérébral sont augmentés pendant la première partie de la crise et redeviennent normaux secondairement. Des lésions ischémiques sont observées dans la zone fragile de l'hippocampe (zone de Sommer) et dans le cortex mais le cervelet reste indemne. 相似文献
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Dina C Meyre D Gallina S Durand E Körner A Jacobson P Carlsson LM Kiess W Vatin V Lecoeur C Delplanque J Vaillant E Pattou F Ruiz J Weill J Levy-Marchal C Horber F Potoczna N Hercberg S Le Stunff C Bougnères P Kovacs P Marre M Balkau B Cauchi S Chèvre JC Froguel P 《Nature genetics》2007,39(6):724-726
We identified a set of SNPs in the first intron of the FTO (fat mass and obesity associated) gene on chromosome 16q12.2 that is consistently strongly associated with early-onset and severe obesity in both adults and children of European ancestry with an experiment-wise P value of 1.67 x 10(-26) in 2,900 affected individuals and 5,100 controls. The at-risk haplotype yields a proportion of attributable risk of 22% for common obesity. We conclude that FTO contributes to human obesity and hence may be a target for subsequent functional analyses. 相似文献
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Effect of different deformation and annealing procedures on non-magnetic textured Cu<Subscript>60</Subscript>Ni<Subscript>40</Subscript> alloy substrates 下载免费PDF全文
Jin Cui Hong-li Suo Jin-hua Wang Jean-Claude Grivel Lin Ma Chun-yan Li Yao-tang Ji Shaheen Kausar Min Liu Yi Wang 《矿物冶金与材料学报》2018,25(8):930-936
In this work, a series of specimens was prepared by the casting method. Sharp cube-textured substrates were processed by heavy cold rolling and recrystallization annealing (i.e., the rolling-assisted biaxially textured substrates (RABiTS) method). Both the rolling and the recrystallization texture in the alloy tapes were investigated by X-ray diffraction and electron back-scatter diffraction, respectively. The results showed that a strong copper-type deformation texture was obtained in the heavy cold-rolled substrate. In addition, the recrystallization annealing process was found to be very important for the texture transition in the Cu–Ni alloy substrates. The cube texture content in the Cu60Ni40 alloy substrates reached 99.7% (≤10°) after optimization of the cold-rolling procedure and the recrystallizing heat-treatment process, whereas the content of low-angle grain boundaries (from 2° to 10° misorientation) in the substrate reached 95.1%. 相似文献
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Reynaud CA Descatoire M Dogan I Huetz F Weller S Weill JC 《Cellular and molecular life sciences : CMLS》2012,69(10):1625-1634
Humoral memory is maintained by two types of persistent cells, memory B cells and plasma cells, which have different phenotypes
and functions. Long-lived plasma cells can survive for a lifespan within a complex niche in the bone marrow and provide continuous
protective serum antibody levels. Memory B cells reside in secondary lymphoid organs, where they can be rapidly mobilized
upon a new antigenic encounter. Surface IgG has long been taken as a surrogate marker for memory in the mouse. Recently, however,
we have brought evidence for a long-lived IgM memory B cell population in the mouse, while we have also argued that, in humans,
these same cells are not classical memory B cells but marginal zone (MZ) B cells which, as opposed to their mouse MZ counterpart,
recirculate and carry a mutated B cell receptor. In this review, we will discuss these apparently paradoxical results. 相似文献
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Charlier C Coppieters W Rollin F Desmecht D Agerholm JS Cambisano N Carta E Dardano S Dive M Fasquelle C Frennet JC Hanset R Hubin X Jorgensen C Karim L Kent M Harvey K Pearce BR Simon P Tama N Nie H Vandeputte S Lien S Longeri M Fredholm M Harvey RJ Georges M 《Nature genetics》2008,40(4):449-454
The widespread use of elite sires by means of artificial insemination in livestock breeding leads to the frequent emergence of recessive genetic defects, which cause significant economic and animal welfare concerns. Here we show that the availability of genome-wide, high-density SNP panels, combined with the typical structure of livestock populations, markedly accelerates the positional identification of genes and mutations that cause inherited defects. We report the fine-scale mapping of five recessive disorders in cattle and the molecular basis for three of these: congenital muscular dystony (CMD) types 1 and 2 in Belgian Blue cattle and ichthyosis fetalis in Italian Chianina cattle. Identification of these causative mutations has an immediate translation into breeding practice, allowing marker assisted selection against the defects through avoidance of at-risk matings. 相似文献
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Jin Cui Hong-li Suo Jin-hua Wang Jean-Claude Grivel Lin Ma Chun-yan Li Yao-tang Ji Shaheen Kausar Min Liu Yi Wang 《北京科技大学学报》2018,(8)
In this work,a series of specimens was prepared by the casting method.Sharp cube-textured substrates were processed by heavy cold rolling and recrystallization annealing(i.e.,the rolling-assisted biaxially textured substrates(RABi TS) method).Both the rolling and the recrystallization texture in the alloy tapes were investigated by X-ray diffraction and electron back-scatter diffraction,respectively.The results showed that a strong copper-type deformation texture was obtained in the heavy cold-rolled substrate.In addition,the recrystallization annealing process was found to be very important for the texture transition in the Cu–Ni alloy substrates.The cube texture content in the Cu60 Ni40 alloy substrates reached 99.7%(≤10°) after optimization of the cold-rolling procedure and the recrystallizing heat-treatment process,whereas the content of low-angle grain boundaries(from 2° to 10° misorientation) in the substrate reached 95.1%. 相似文献
9.
Effect of the synthesis route on the structural and dielectric properties of SrBi<Subscript>1.8</Subscript>Y<Subscript>0.2</Subscript>Nb<Subscript>2</Subscript>O<Subscript>9</Subscript> ceramics 下载免费PDF全文
Mohamed Afqir Amina Tachafine Didier Fasquelle Mohamed Elaatmani Jean-Claude Carru Abdelouahad Zegzouti Mustapha El Hammioui 《矿物冶金与材料学报》2018,25(11):1304-1312
This work is devoted to the synthesis and characterization of yttrium-doped SrBi2Nb2O9 ceramics prepared by three methods: solid state reaction, co-precipitation, and hydrothermal. Multiple characterizations, specifically scanning electron microscopy (SEM), X-ray powder diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR), were used to validate the structural feature. The crystallite size was estimated by Scherrer’s formula and the Williamson–Hall plot. The effect of the process on the band intensities of the FTIR spectra was investigated. The crystallite size and microstructure of ceramics prepared from different synthesis processes were strongly influenced by the sinterability. SEM images revealed nanograin ceramics for materials prepared by co-precipitation and hydrothermal methods and micrograin ceramics prepared by the solid state method. The synthesized compounds underwent phase transitions at 480–465°C. The dielectric and electrical properties of these Y-doped SrBi2Nb2O9 ceramics appear to be dependent on the grain size. 相似文献
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Pryor WR Rymer AM Mitchell DG Hill TW Young DT Saur J Jones GH Jacobsen S Cowley SW Mauk BH Coates AJ Gustin J Grodent D Gérard JC Lamy L Nichols JD Krimigis SM Esposito LW Dougherty MK Jouchoux AJ Stewart AI McClintock WE Holsclaw GM Ajello JM Colwell JE Hendrix AR Crary FJ Clarke JT Zhou X 《Nature》2011,472(7343):331-333
Although there are substantial differences between the magnetospheres of Jupiter and Saturn, it has been suggested that cryovolcanic activity at Enceladus could lead to electrodynamic coupling between Enceladus and Saturn like that which links Jupiter with Io, Europa and Ganymede. Powerful field-aligned electron beams associated with the Io-Jupiter coupling, for example, create an auroral footprint in Jupiter's ionosphere. Auroral ultraviolet emission associated with Enceladus-Saturn coupling is anticipated to be just a few tenths of a kilorayleigh (ref. 12), about an order of magnitude dimmer than Io's footprint and below the observable threshold, consistent with its non-detection. Here we report the detection of magnetic-field-aligned ion and electron beams (offset several moon radii downstream from Enceladus) with sufficient power to stimulate detectable aurora, and the subsequent discovery of Enceladus-associated aurora in a few per cent of the scans of the moon's footprint. The footprint varies in emission magnitude more than can plausibly be explained by changes in magnetospheric parameters--and as such is probably indicative of variable plume activity. 相似文献