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1.
A topoisomerase II inhibitor, novobiocin, and a deacetylase inhibitor, butyrate, synergistically transformed human liver cells into fibroblast-like cells. This morphological change was associated with an increased production of procollagen type III peptide and a simultaneous assembly of actin, tubulin, vimentin and cytokeratin. Novobiocin and butyrate had no marked effect on the phosphorylation state of cytokeratin proteins, but synergistically enhanced [3H]acetate uptake. From these results, it can be speculated that protein acetylation plays an important role in inducing the assembly of cytoskeletal proteins and the morphological transformation of human liver cells. 相似文献
2.
基于SIMULINK的Fuzzy-PID控制器设计 总被引:1,自引:0,他引:1
设计了一种Fuzzy-PID控制算法,利用MATLAB的SIMULINK进行仿真研究,结果表明,该控制器具有较强的抗干扰能力和鲁棒性,有较好的动、静态控制精度. 相似文献
3.
Hypothalamic histamine modulates adaptive behavior of rats at high environmental temperature 总被引:1,自引:0,他引:1
K. Fujimoto T. Sakata K. Ookuma M. Kurokawa A. Yamatodani H. Wada 《Cellular and molecular life sciences : CMLS》1990,46(3):283-285
Summary Histamine content in the rat hypothalamus was lower at 4°C and higher at 31°C compared to that at 21°C. Pretreatment with -fluoromethylhistidine, a suicide inhibitor of histidine decarboxylase, attenuated both the increased level of hypothalamic histamine and rat adaptive behavior at 31°C. Increase of histamine content in the hypothalamus appears to be an important factor contributing to rat adaptive behavior to high environmental temperature. 相似文献
4.
5.
受到资讯媒体新科技影响 ,图书馆读者使用行为已从单一的馆藏利用 ,转为期望图书馆提供更多元化的服务 ;另外在财政紧缩 资源扩增 人力不足等因素下 ,图书馆提供各类型服务的运作模式也受到相当的冲击 ,图书馆为提供以读者为道向的服务 ,必需付出许多努力并改变服务哲学 ,因而图书馆馆员的专业知能发展要不断提升才能因应数位时代的环境改变 ,并提供读者一个优质的资讯服务 ,发挥图书馆LearningResourceCenter学习资源中心的角色 .本研究主要探讨图书馆如何在有限资源下 ,有系统地规划发展馆员专业知能 ,并且利用那一种方法可以最有效率、花费最少成本达到最大的馆员能力提升效果 ,根据文献探讨以及学习行为方法理论 ,提出一个适合发展馆员专业知能的模式 (Approachmodel) ,通过探讨逢甲大学图书馆的两个专案其馆员专业知能转变的历程 ,检视了此发展模式的成效 ,并提出未来该模式可再改进的方向及建议 相似文献
6.
Anne Berna François Bernier Eric Chabrière Mikael Elias Ken Scott Andrew Suh 《Cellular and molecular life sciences : CMLS》2009,66(14):2205-2218
DING proteins, identified mainly by their eponymous N-terminal sequences, are ubiquitous in living organisms. Amongst bacteria,
they are common in pseudomonads, and have been characterised with respect to genetics and structure. They form part of a wider
family of phosphate-binding proteins, with emerging roles in phosphate acquisition and pathogenicity. Many DING proteins have
been isolated in eukaryotes, in which they have been associated with very diverse biological activities, often in the context
of possible signalling roles. Disease states in which DING proteins have been implicated include rheumatoid arthritis, lithiasis,
atherosclerosis, some tumours and tumour-associated cachexia, and bacterial and viral adherence. Complete genetic and structural
characterisation of eukaryotic DING genes and proteins is still lacking, though the phosphate-binding site seems to be conserved.
Whether as bacterial proteins related to bacterial pathogenicity, or as eukaryotic components of biochemical signalling systems,
DING proteins require further study.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
7.
Sato T Mushiake S Kato Y Sato K Sato M Takeda N Ozono K Miki K Kubo Y Tsuji A Harada R Harada A 《Nature》2007,448(7151):366-369
A number of proteins are known to be involved in apical/basolateral transport of proteins in polarized epithelial cells. The small GTP-binding protein Rab8 was thought to regulate basolateral transport in polarized kidney epithelial cells through the AP1B-complex-mediated pathway. However, the role of Rab8 (Rab8A) in cell polarity in vivo remains unknown. Here we show that Rab8 is responsible for the localization of apical proteins in intestinal epithelial cells. We found that apical peptidases and transporters localized to lysosomes in the small intestine of Rab8-deficient mice. Their mislocalization and degradation in lysosomes led to a marked reduction in the absorption rate of nutrients in the small intestine, and ultimately to death. Ultrastructurally, a shortening of apical microvilli, an increased number of enlarged lysosomes, and microvillus inclusions in the enterocytes were also observed. One microvillus inclusion disease patient who shows an identical phenotype to Rab8-deficient mice expresses a reduced amount of RAB8 (RAB8A; NM_005370). Our results demonstrate that Rab8 is necessary for the proper localization of apical proteins and the absorption and digestion of various nutrients in the small intestine. 相似文献
8.
一种新型高速码垛机械手的设计与实现 总被引:13,自引:0,他引:13
针对包装袋规格和码垛技术要求,提出一种新型的高速码垛机械手的设计方案.该方案采用气压驱动,并通过手指开合机构、侧板夹紧机构和压袋机构,实现包装袋的可靠抓包、搬运和码放等动作.在此基础上,通过详细分析与计算,完成了该机械手的结构设计,并在Pro/E平台中建立了该机械手的三维CAD模型.对该机械手的动作规划、动力学计算以及气动系统等问题进行了分析与研究.仿真结果表明,该机械手能够在规定的时间内完成规定动作. 相似文献
9.
Electrons moving in two dimensions under the influence of strong magnetic fields effectively lose their kinetic energy and display exotic behaviour dominated by Coulomb forces. When the ratio of electrons to magnetic flux quanta in the system (ν) is near 5/2, the electrons are predicted to condense into a correlated phase with fractionally charged quasiparticles and a ground-state degeneracy that grows exponentially as these quasiparticles are introduced. The only way for electrons to transform between the many ground states would be to braid the fractional excitations around each other. This property has been proposed as the basis of a fault-tolerant quantum computer. Here we present observations of localized quasiparticles at ν = 5/2, confined to puddles by disorder. Using a local electrometer to compare how quasiparticles at ν = 5/2 and ν = 7/3 charge these puddles, we were able to extract the ratio of local charges for these states. Averaged over several disorder configurations and samples, we found the ratio to be 4/3, suggesting that the local charges are = e/3 and = e/4, where e is the charge of an electron. This is in agreement with theoretical predictions for a paired state at ν = 5/2. Confirming the existence of localized e/4 quasiparticles shows that proposed interferometry experiments to test statistics and computational ability of the state at ν = 5/2 would be possible. 相似文献
10.
Don't judge species on their origins 总被引:1,自引:0,他引:1