Structural and expressional analysis of a cDNA that expresses predominantly in rice stamens

The development of stamen is an important stage during the sexual reproduction in higher plants. Studying the genes that predominantly or even specifically expressed in stamens will aid in undestanding the molecular mechanism of stamen development. The structure of a rice CHS-like gene (D5) which predominantly expressed in anthers has been analysised, showing high structural similarity with two other anther-specific genes (A1 and BA42) fromBrassica napus. Northern analysis also reveals that the three genes share a similar expression pattern and that the expression of D5 may relate to the early development of pollens. It is concluded that D5, A1 and BA42 represent a group of diverged CHS-like genes which are related to stamen development.     

偶维数拟正则映照的一点注记

给出了偶维数下的外幂算子与Ｈｏｄｇｅ星算子可交换的充要条件，并给出矩阵Ａ与其外幂矩阵之间的关系。     

(E)-3-芳亚甲基色满酮类化合物的快速合成及其抑真菌活性

在微波辐射下,通过色满酮与芳香醛的缩合反应,快速合成了12个新的(E)-3-芳亚甲基色满酮类化合物2a～2l.所有化合物结构均经IR,1 H NMR,13 C NMR及元素分析确认.初步的抑菌活性测定结果表明:目标化合物在质量浓度100mg/L下对5种供试的植物病原真菌表现出不同程度的抑菌活性.其中化合物(E)-6-氯-3-(2,4-二氯苯基)亚甲基色满酮2c对梨黑斑病菌抑制活性最强,抑制率为68.2%.     

石珊瑚起源的形态学和分子系统学研究

石珊瑚是现代最重要的造礁动物,在地质史的过去和现在都非常重要。石珊瑚的起源一直是珊瑚研究中颇有争议的重大问题：石珊瑚究竟是起源于古生代的皱壁珊瑚、一种石珊瑚形珊瑚,还是一类软体海葵,目前还很难下定论。建立在现代石珊瑚及其化石的形态学特征基础上的传统研究手段和新兴的分子系统学分析手段,是研究石珊瑚起源问题的2种主要方法。用分子生物学方法测定了珊瑚的18S rDNA,构建了珊瑚纲珊瑚的系统发育树,对当前国际上争论纷纷的有关石珊瑚起源问题进行了形态学和分子系统学的比较研究,以期为更好地解决石珊瑚起源问题提供思路。     

基于灰度跳变与形态学的车牌定位法

车牌定位是车牌识别系统的关键步骤,定位的准确与否直接影响车牌识别的结果.提出了一种基于灰度跳变与形态学的定位方法.对于车牌候选区的二值图像,根据车牌的水平分布特点,从长宽之比、面积之比等多个方面进行综合分析,从而可以更加精确地对车牌进行识别.实验验证表明,基于该算法的车牌识别系统能够达到较高的车牌识别率.     

一些新的双边不等式

不等式出现于数学的各个分支,并广泛应用于数学、物理学、力学和工程技术领域.给出了双边不等式成立的一个充分条件,并得到了几个与Seiffert平均、广义海伦平均、算术平均和加权几何平均有关的新的双边不等式.     

近2000年来太白山高山带不同海拔植被对气候变化的响应

根据西佛爷池(海拔3410 m)剖面的有机地球化学指标, 重建太白山高山带过去2000年的气候变化。对采自该剖面的样品进行孢粉分析, 并结合太白山跑马梁(海拔3556 m)、三清池(海拔3080 m)和芳香寺(海拔3000 m)剖面的孢粉数据, 进行聚类分析, 探讨高山带不同海拔植被对气候变化响应规律。结果表明, 不同海拔植被对相对冷干气候的响应有更好的一致性, 且林线过渡带内这种一致性更明显。随着现代升温期的到来, 植被对气候的响应主要受高程影响。研究结果可为认识晚全新世以来高山环境系统的气候?植被耦合关系提供一定的参考。     

Inducible expression pattern of rice Bowman-Birk inhibitor gene<Emphasis Type="Italic">OsWIP1-2</Emphasis> and its protease inhibitory activity

The WIP1-2 gene was cloned from rice. It be-longs to the Bowman-Birk inhibitor gene family. Northernblot showed that expression of this gene was induced bywounding and jasmonic acid (JA). It indicates that the OsWIPI gene plays an important role in the rice defense sys-tem. The OsWIP1-2 was cloned into pET28a and expressed inE. coli. Its expressed product was purified in the form offusion protein and tested for the inhibitory activities againsttrypsin and chymotrypsin. It was found that the fusion pro-tein could inhibit chymotrypsin, but not trypsin. It was alsofound that the His tag at its C-terminal affected its inhibitoryactivity significantly. The fusion protein with a naturalC-terminal had the inhibitory activity, while no inhibitoryactivity was detected in the fusion protein with a (His)6-tag atits C-terminal. This implies that extra amino acid residues atthe C-terminal of OsWIP1-2 may interfere with its correctfolding. The inhibitory assay indicated that the members ofrice Bowman-Birk inhibitor gene family probably differenti-ated both in their structure and function.     

一阶线性椭圆型偏微分方程组的R-H-DH-D2H复合边值问题

研究了一阶线性椭圆型偏微分方程组的边界条件中含有二阶偏导数的R-H-DH-D^2H复合边值问题，利用消去法将该问题化为等价的广义解析向量的Hilbert边值问题，并利用奇异积分方程组理论给出了问题的可解性条件．