索科线虫RAPD反应条件优化

以中华卵索线虫为材料,研究索科线虫DNA的提取以及对RAPD的影响因素,包括模板DNA、Mg2+、dNTP、引物和Taq聚合酶浓度等,探索最佳反应条件,构建了适于索科线虫RAPD分析的稳定的反应体系.25μL反应体系中,含模板DNA20~40 ng,10×PCR缓冲液2.5μL,MgCl22.0 mmol/L,dNTP 150μmol/L,引物0.3μmol/L,Taq聚合酶1U.实验证明,在对索科线虫进行RAPD分析时,采用优化的反应条件,规范实验操作,使用同厂家同批次的试剂,实验结果就会具有很好的重复性和稳定性.     

喷雾场激光全息照像微机控制系统

研制了一种喷雾场激光全息照像微机控制系统．该系统电机测速调速精度达到±１ｒ／ｍｉｎ，光开关开启绝对误差小于０．０２５°转角（１μｓ时差）．近３年的实践证明该系统工作稳定可靠．     

蒲圻陆水蝶类资源调查初报：Ⅱ，粉蝶科、眼蝶科、灰蝶科、弄蝶科

在湖北省蒲圻市陆水水库风景保护区共采集到粉蝶科蝴蝶8属14种，眼蝶科6属13种，灰蝶科12属12种，弄蝶科7属8种，其中16种为湖北省分布新记录，并记述了各种蝴蝶的学名，寄主和区系组成。     

基于时频单源点检测的雷达信号的盲分选

针对欠定条件下的雷达信号分选问题,提出一种基于时频单源点检测的雷达信号盲分选算法,该分选算法的创新点在于将雷达信号由时域转移到时频域进行分析.在时频域内,雷达信号具有一定的稀疏性,有助于实现信号盲分选,给出了基于时频单源点检测的雷达信号盲分选算法的具体步骤.该方法能够有效解决欠定条件下雷达信号盲分选问题,将其应用于雷达信号分选领域,具有一定的军事应用价值.仿真测试结果表明了该方法的可行性与优越性.     

略论高校图书馆期刊文献的收集与开发利用

知识经济时代高校图书馆的期刊工作十分重要,结合本校图书馆实际,对期刊文献的采购,收藏与开发利用进行了探讨。     

极端气候下湖南省某黏土滑坡特征与防治

湖南省地质灾害多发,且多为与斜坡稳定性相关的崩滑流。湖南省某黏土滑坡自2016年2月初发生局部滑动与垮塌等现象,后续持续发展。本文通过分析该滑坡特征和变形破坏成因,得出如下结论:边坡的初始变形破坏的形成因素包括地形地貌因素、岩性因素、持续强降雨、人类工程活动及极端气候下的冻融作用等;夏季暴雨期间变形加剧主要与强降雨有关;采用截排水工程、削方减载工程、坡脚挡土墙工程和监测工程相结合对该滑坡进行防治。     

改变传统观念促进优质服务

图书馆服务工作的出发点和归宿是为了搞好服务工作，不仅要有良好的办馆条件和结构藏书体系，而且要更新服务方式，提高服务质量，讲究服务效益。     

Numerical modelling of a turbulent bluff-body flow with Reynolds stress turbulent models

A bluff body stabilized flow has received specialattention recently[1—4]. In addition to its practical in terest, the bluff body flow is an excellent challengingtest case for turbulence model due to its simple andwell defined initial and boundary conditions with thecomplex recirculating flows.The bluff body stabilized flow investigated herewas studied experimentally by Dally et al.[1,2] As forits numerical simulation, Dally et al.[1] reported sim ulation results obtained using standard and …     

Tailoring impedance match and enhancing microwave absorption of Fe3O4/ Bi24Fe2O39/Bi hollow porous microrods by controlling their composition

A self-assembly/precipitate conversion/decomposition process was developed for the controllable synthesis of Fe_3O_4/Bi_(24)Fe_2O_(39)/Bi hollow porous microrods(HPMRs).The results demonstrated that the crystal size,component,and performances of HPMRs could be effectively modulated via changing Fe~(2+)/Bi~(3+)molar ratio(γ).Fe_3O_4/Bi_(24)Fe_2O_(39)/Bi HPMRs exhibited ferromagnetic behavior at room temperature.As Bi and Bi_(24)Fe_2O_(39)contents increased withγ,the saturation magnetization M_sand attenuation constantly decreased,whereas coercivity H_cand impedance matching ratio increased.Compounding Fe_3O_4with small quantities of Bi and Bi_(24)Fe_2O_(39)into HPMRs can significantly enhance microwave absorption.Fe_3O_4/Bi_(24)Fe_2O_(39)/Bi HPMRs formed atγ=1:0.25 exhibited the optimum microwave absorption performance.The minimum R_Lwas-47.3 dB at8.72 GHz,corresponding to 2.4 mm sample thickness.The absorption band with the reflection loss below-20 dB was up to 14.0 GHz for the absorber with a thickness of 1.4-8.0 mm.The results demonstrate that the introduction of electromagnetic transparent materials(Bi_(24)Fe_2O_(39)or Bi)can improve the microwave absorption performances of Fe_3O_4 composites owing to enhanced impedance matching rather than attenuation constant.