Fabrication and Characterization of Chitosan Nerve Conduits with Microtubular Architectures

Porous multi-channel chitosan conduits were fabricated using a novel phase-separation technique with an axial temperature gradient. First, porous chitosan tubes were made with a mold that was composed of two concentric polytetrafiuoroethylene tubes. Then 1%-3% （w/v） chitosan solution was injected into the chitosan tube while the two ends of the tube were closed with steel rods. Then the outside of the tube was wrapped with a layer of thermal insulating material to reduce the heat transfer through the outside, and the tubes were placed in a freezer. The resulting phase separation then occurred in the presence of an axial temperature gradient. The porosity, microtubule diameter, and orientation were controlled by adjusting the polymer concentration and temperature gradient. After the preparation course, no poisonous substances remained on the conduits. The mechanical properties, swelling, and biodegradability of the chitosan conduits were investigated, and a scanning electron microscope was used to observe the tubular morphology and growth of neuroblastoma cells （N2A, mouse） in the conduits. The results demonstrate that the multi-channel chitosan conduits have suitable mechanical strength, swelling, degradation properties, and nerve cell affinity, so they hold promise for use as neural tissue engineering scaffolds.     

Neural Stem Cell Affinity of Chitosan and Feasibility of ChitosanBased Porous Conduits as Scaffolds for Nerve Tissue Engineering

Introduction Neural stem cells (NSCs) are known to have both self- proliferation potential and multiple differentiation po- tential[1]. Previous studies have shown that NSCs can differentiate into various cell types such as neurons, as- trocytes, oligoden…     

促进周围神经再生的药物研究进展

周围神经损伤是目前临床上常见的创伤并发症.促进周围神经损伤后的再生,恢复其功能已日益成为研究的热点.近年来,不少学者做了药物促进周围神经再生的研究并取得了一定效果.本文将近年来临床与实验研究中常用促进周围神经再生的药物做一综述.     

In Vitro Cytotoxicity and Protein Drug Release Properties of Chitosan/Heparin Mlcrospheres

Chitosan/heparin microspheres were prepared using the water-in-oil emulsification solvent evaporation technique. The microsphere diameters were controlled by selecting the fabrication process parameters. Scanning electron micrographs showed that the chitosan/heparin microspheres were regular and the surface morphology was smooth. Fourier transform infrared showed that the chitosan amino groups reacted with heparin carboxylic groups to form acylamides in the microspheres. Analysis of the microsphere cytotoxicity showed that they had no cytotoxic effect and behaved very similar to the negative control （polystyrene）. To analyze the protein drug release profiles of the microspheres, bovine serum albumin was loaded as a model drug into the microspheres and released in vitro. Marked retardation was observed in the BSA release profiles. The results show that chitosan/heparin microspheres may provide a useful controlled release protein drug system for used in pharmaceutics.     

骨形态发生蛋白研究进展

本文对骨形态发生蛋白的分子生物学研究状况、骨形态发生蛋白的载体及其促进骨形成机理和临床应用等方面进行了阐述,并提出了骨形态发生蛋白在临床应用中存在的问题及应用前景.     

如何提高学生的数学学习效率

在我看来,数学是一门规律性、技巧性很强的学科,所以在教学过程中教师所起到的作用是有限的,尤其是在课堂教学过程中,学生的听课效果往往对其学习效果起决定性的作用。因此,我们在数学教学过程中最主要的任务是培养学生的自学能力,提高学生的学习效率。     

Fiber-Based Chitosan Tubular Scaffolds for Soft Tissue Engineering： Fabrication and in Vitro Evaluation

Porous, two-ply tubular chitosan conduits for guided tissue regeneration were fabricated by combining the textile technique （inner layer） with the thermally induced phase separation process （outer layer）. A hollow chitosan tube was prepared using an industrial warp knitting process with chitosan yarns. Then, an appropriate diameter mandrel was inserted into the pre-fabricated tube. The tube and the mandrel were dipped into the chitosan solution together, taken out, and freeze-dried. After being neutralized in alkaline solution and dried at room temperature, the mandrel was removed to create the chitosan tubular scaffold. Scanning electron micrographs show that the resulting tubes have a biphasic wall structure, with a fibrous inner layer and a semipermeable outer layer. The swelling properties and the mechanical strength before and after in vitro degradation were investigated. The biocompatibility of the scaffolds was also investigated by co-culturing neuroblastoma cells （N2A, mouse） with the scaffolds. The results suggest that these chitosan tubular scaffolds are useful for the regeneration of tissues requiring a tubular scaffold.     

薄壁轴承安装座工艺研究

轴承安装座为薄壁复杂件,外形尺寸较大,大端面壁厚不到3mm。内孔和外圆尺寸精度较高,技术条件要求极为严格,工艺制造的过程中很难加工保证,需要制定合理的工艺方案,研究解决薄壁型面的大端面、内孔加工变形以及内孔在铣加工深环槽后的变形问题,保证外圆、内孔尺寸及技术条件是技术难点。针对工件结构特点分析出端面、内孔变形原因,增加了相关工序内容、自制了关键工装、选择了合理的切削参数,最终顺利攻克这一难题。     

In Vitro Cytotoxicity and Protein Drug Release Properties of Chitosan/Heparin Microspheres

Chitosan/heparin microspheres were prepared using the water-in-oil emulsification solvent evapo- ration technique. The microsphere diameters were controlled by selecting the fabrication process parame- ters. Scanning electron micrographs showed that the chitosan/heparin microspheres were regular and the surface morphology was smooth. Fourier transform infrared showed that the chitosan amino groups reacted with heparin carboxylic groups to form acylamides in the microspheres. Analysis of the microsphere cytotox- icity showed that they had no cytotoxic effect and behaved very similar to the negative control (polystyrene). To analyze the protein drug release profiles of the microspheres, bovine serum albumin was loaded as a model drug into the microspheres and released in vitro. Marked retardation was observed in the BSA re- lease profiles. The results show that chitosan/heparin microspheres may provide a useful controlled release protein drug system for used in pharmaceutics.     

基于极点分析方法的晶体振荡器电路设计及仿真

提出了一种利用Hspice软件进行极点数据分析去设定振荡器电路参数值的方法.利用极点与系统稳定性的关系,结合振荡器的工作过程,只要选取合适的电路参数将整个系统的极点设置在一个恰当的位置,振荡器在最终瞬态仿真中的参数就比较理想.这种分析设计方法的好处是避免了反复进行电路的瞬态仿真,大大节省了电路设计的时间.利用极点分析的方法,也可以进行其他复杂电路的稳定性分析.