Sirtuin activators mimic caloric restriction and delay ageing in metazoans

Caloric restriction extends lifespan in numerous species. In the budding yeast Saccharomyces cerevisiae this effect requires Sir2 (ref. 1), a member of the sirtuin family of NAD+-dependent deacetylases. Sirtuin activating compounds (STACs) can promote the survival of human cells and extend the replicative lifespan of yeast. Here we show that resveratrol and other STACs activate sirtuins from Caenorhabditis elegans and Drosophila melanogaster, and extend the lifespan of these animals without reducing fecundity. Lifespan extension is dependent on functional Sir2, and is not observed when nutrients are restricted. Together these data indicate that STACs slow metazoan ageing by mechanisms that may be related to caloric restriction.     

New synonymy and new species of American bark beetles (Coleoptera: Scolytidae)

New replacement names for junior homonyms are presented as follows; Acanthotomicus tuberculifer for A. (Mimips) tuberculatus Schedl 1967, Chactoptelius versicolor for C. (Acrantus) tricolor Schedl 1958, Hylesinopsis angolanus for H. (Aridiamerus) angolensis Schedl 1982, Hylurgops tuberculifer for H. tuberculatus Schedl 1947, Pseudothysanoes spinatifer for P. spinatus Wood 1956, Scolytodes aterrimus for S. ater (Hylocurosoma atrum Eggers) 1941, Scolytodes boliviensis for S. (Prionosceles) bolivianus Eggers 1928, Scolytodes brasiliensis for S. (Hexacolus) brasiliensis Schedl 1935, Scolytodes discriminatus for S. discedens Eggers 1943, Scolytodes elongatissimus for S. elongatus (Hylocurosoma elongatum Eggers) 1943, Scolytodes gennaens for S. genialis Wood 1978, Scolytodes laevigatulus for S. (Hexacolus) laevigatus Schedl 1962, Scolytodes laevicorpus for S. laevis (Hylocurosoma laeve Eggers) 1943, Scolytodes majus for S. major Eggers 1943, Scolytodes medialis for S. medius Eggers 1943. New synonymy is reported for Dactylipalpus niger Schedl (= D. unctus Wood), Dendroctonus armandi Tsai & Li (= D. prosorovi Kurenzov & Kononov), Sinophloeus porter Brèthes (= S. destructor Eggers). Species new to science include: Acacicis bicornis (New Guinea), Acacicis zeylanicus (Sri Lanka), Sphaerotrypes bengalensis (India), Sphaerotrypes costatus (North Andaman Island), Sphaerotrypes cristatus (Sri Lanka), Sphaerotrypes pentacme (Burma), Sphaerotrypes ranasinghei (Sri Lanka), Xylechinus ougeiniac (India), Xylechinus padus (India).     

Terrestrial vertebrate fauna of the Kaiparowits Basin

This report includes data collected during an investigation by Brigham Young University personnel from 1971 to 1976, as well as a literature review. The fauna of the Kaiparowits Basin is represented by 7 species of amphibians (1 salamander, 5 toads, and 1 tree frog), 29 species of reptiles (1 turtle, 16 lizards, and 12 snakes), 183 species of birds (plus 2 hypothetical), and 74 species of mammals. Geographic distribution of the various species within the basin are discussed. Birds are categorized according to their population and seasonal status. Avian habitat relationships are discussed, and extensions of range are reported for 5 species of birds. Three threatened or endangered avian species occur in the basin. Four avian species seem to have declined significantly in numbers in recent years.     

New synonymy and new species of American bark beetles (Coleoptera: Scolytidae), Part III

New synonomy is proposed as follows: Metacorthylus Blandford (= Paracorthylus Wood), Corthylus spinifer Schwarz (= Corthylus tomentosus Schedl), Cryptocarinus brevicollis Eggers ( = Cryptocarenus coronatus Wood), Dendrocranulus guatemalensis (Hopkins), n. comb. (= Dendrocranulus parallelus Schedl), Dendroterus mexicanus Blandford (= Conophthocranulus umbratus Schedl), Hypothenemus cylindricus (Hopkins) (= Hypothenemus guadeloupensis Schedl), Hypothenemus erectus LeConte (= Stephanoderes discedens Schedl), Hypothenemus javanus (Eggers) (= Stephanoderes pistor Schedl, Stephanoderes prosper Schedl), Xyleborus pseudotenuis Schedl (= Xyleborus tenuis Schedl), and Xyleborus villosulus Blandford (= Xyleborus coccotrypoides Eggers, villosus Schedl). Scolytodes punctiferus n. n. is proposed for Scolytodes punctifer Wood, 1971 (nec Wood 1969). The following species are named as new to science: Amphicranus argutus, A. fulgidus, A. micans, Pityophthorus inops, P. debilis, P. strictus. P. galeritus. P. sobrinus, P. laetus, P. lenis, P. conspectus P. medialis (Costa Rica), P. perexiguus (Costa Rica and Panama), P. scitulus (Panama), P. costatulus, P. costabilis, P. detentus, P. nebulosus, P. melanurus, P. indigens, P. burserae, P. molestus, P. diligens, P. corruptus (Mexico), P. tenax, P. nugalis, P. minutalis (Guatemala), P. nemoralis, P. morosus, P. hermosus (Honduras), Araptus gracilens (Mexico).     

Relict occurrence of three "American" Scolytidae (Coleoptera) in Asia

The first non-American representatives of the bark beetle genus Pseudopityophthorus , of the subtribe Corthylina (Corthylini), and of the Ips concinnus species group are named. All are apparently relicts of an early faunal exchange with North America. The new taxa include: Gnatharus , new genus, and its type-species Gnatharus tibetensis , new species (Tibet); Xenophthorus , new subgenus, and its type-species Pseudopityophthorus (Xenophthorus) peregrinus , new species (Tibet), and Ips orientalis , new species (Tibet, China). The intercontinental exchange of other taxa in Scolytidae are also mentioned.     

Multimodal fast optical interrogation of neural circuitry

Our understanding of the cellular implementation of systems-level neural processes like action, thought and emotion has been limited by the availability of tools to interrogate specific classes of neural cells within intact, living brain tissue. Here we identify and develop an archaeal light-driven chloride pump (NpHR) from Natronomonas pharaonis for temporally precise optical inhibition of neural activity. NpHR allows either knockout of single action potentials, or sustained blockade of spiking. NpHR is compatible with ChR2, the previous optical excitation technology we have described, in that the two opposing probes operate at similar light powers but with well-separated action spectra. NpHR, like ChR2, functions in mammals without exogenous cofactors, and the two probes can be integrated with calcium imaging in mammalian brain tissue for bidirectional optical modulation and readout of neural activity. Likewise, NpHR and ChR2 can be targeted together to Caenorhabditis elegans muscle and cholinergic motor neurons to control locomotion bidirectionally. NpHR and ChR2 form a complete system for multimodal, high-speed, genetically targeted, all-optical interrogation of living neural circuits.     

A draft genome of Yersinia pestis from victims of the Black Death

Technological advances in DNA recovery and sequencing have drastically expanded the scope of genetic analyses of ancient specimens to the extent that full genomic investigations are now feasible and are quickly becoming standard. This trend has important implications for infectious disease research because genomic data from ancient microbes may help to elucidate mechanisms of pathogen evolution and adaptation for emerging and re-emerging infections. Here we report a reconstructed ancient genome of Yersinia pestis at 30-fold average coverage from Black Death victims securely dated to episodes of pestilence-associated mortality in London, England, 1348-1350. Genetic architecture and phylogenetic analysis indicate that the ancient organism is ancestral to most extant strains and sits very close to the ancestral node of all Y. pestis commonly associated with human infection. Temporal estimates suggest that the Black Death of 1347-1351 was the main historical event responsible for the introduction and widespread dissemination of the ancestor to all currently circulating Y. pestis strains pathogenic to humans, and further indicates that contemporary Y. pestis epidemics have their origins in the medieval era. Comparisons against modern genomes reveal no unique derived positions in the medieval organism, indicating that the perceived increased virulence of the disease during the Black Death may not have been due to bacterial phenotype. These findings support the notion that factors other than microbial genetics, such as environment, vector dynamics and host susceptibility, should be at the forefront of epidemiological discussions regarding emerging Y. pestis infections.