P?un’s systems as models of economic systems

In the paper a concept for modeling the economic systems by the formalism of P?un’s systems is proposed. The main idea is to represent economic systems as homomorphic images of Petri nets corresponding to the (super) cells of P?un’s systems.     

Minimal Sample Size in the Group Classification Problem

Bayes classification procedure for a group of independent vectors treated as a whole is considered. When the distributions are not specified, we obtain the bounds of the minimal sample size based on the Chernoff and the Bhattacharyya distances between the populations. The case of the normal distribution is also discussed.     

P?un’s systems as models of economic systems

In the paper a concept for modeling the economic systems by the formalism of P?un’s systems is proposed. The main idea is to represent economic systems as homomorphic images of Petri nets corresponding to the (super) cells of P?un’s systems.     

Pǎun''''s systems as models of economic systems

In the paper a concept for modeling the economic systems by the formalism of Paun's systems is proposed. The main idea is to represent economic systems as homomorphic images of Petri nets corresponding to the (super) cells of Paun's systems.     

Type VI secretion delivers bacteriolytic effectors to target cells

Peptidoglycan is the major structural constituent of the bacterial cell wall, forming a meshwork outside the cytoplasmic membrane that maintains cell shape and prevents lysis. In Gram-negative bacteria, peptidoglycan is located in the periplasm, where it is protected from exogenous lytic enzymes by the outer membrane. Here we show that the type VI secretion system of Pseudomonas aeruginosa breaches this barrier to deliver two effector proteins, Tse1 and Tse3, to the periplasm of recipient cells. In this compartment, the effectors hydrolyse peptidoglycan, thereby providing a fitness advantage for P. aeruginosa cells in competition with other bacteria. To protect itself from lysis by Tse1 and Tse3, P. aeruginosa uses specific periplasmically localized immunity proteins. The requirement for these immunity proteins depends on intercellular self-intoxication through an active type VI secretion system, indicating a mechanism for export whereby effectors do not access donor cell periplasm in transit.     

Inhibition of cytohesins by SecinH3 leads to hepatic insulin resistance

G proteins are an important class of regulatory switches in all living systems. They are activated by guanine nucleotide exchange factors (GEFs), which facilitate the exchange of GDP for GTP. This activity makes GEFs attractive targets for modulating disease-relevant G-protein-controlled signalling networks. GEF inhibitors are therefore of interest as tools for elucidating the function of these proteins and for therapeutic intervention; however, only one small molecule GEF inhibitor, brefeldin A (BFA), is currently available. Here we used an aptamer displacement screen to identify SecinH3, a small molecule antagonist of cytohesins. The cytohesins are a class of BFA-resistant small GEFs for ADP-ribosylation factors (ARFs), which regulate cytoskeletal organization, integrin activation or integrin signalling. The application of SecinH3 in human liver cells showed that insulin-receptor-complex-associated cytohesins are required for insulin signalling. SecinH3-treated mice show increased expression of gluconeogenic genes, reduced expression of glycolytic, fatty acid and ketone body metabolism genes in the liver, reduced liver glycogen stores, and a compensatory increase in plasma insulin. Thus, cytohesin inhibition results in hepatic insulin resistance. Because insulin resistance is among the earliest pathological changes in type 2 diabetes, our results show the potential of chemical biology for dissecting the molecular pathogenesis of this disease.