Retrograde transport of endocytosed Shiga toxin to the endoplasmic reticulum.

Shiga toxin and some other protein toxins that act on targets in the cytosol have previously been shown to enter the trans-Golgi network. Transport by this route may be necessary for translocation of the toxin to the cytosol and for intoxication, but it is not known whether the enzymatically active part of the toxins actually enters the cytosol from the trans-Golgi network. It has been suggested that such toxins are transported in a retrograde manner to the endoplasmic reticulum and that translocation occurs in this organelle, but retrograde transport of endocytosed material beyond the trans-Golgi network has never been demonstrated. Here we show that in butyric acid-treated A431 cells endocytosed Shiga toxin is not only transported to the trans-Golgi network, but also to all Golgi stacks, to the endoplasmic reticulum and to the nuclear envelope. Furthermore, butyric acid sensitizes the cells to Shiga toxin, which is consistent with the possibility that retrograde transport is required for translocation of the toxin to the cytosol.     

探讨使用野外随机取样推断随机变量概率分布的数学方法--杂草研究作为一个例子

我们在生态学研究中涉及到的大多数变量呈现出随机性，因而可以认为它们就是随机变量。概率分布是研究随机变量变化规律的基础，如何利用野外取样来推断检验随机变量的分布是本文要解决的主要问题。本文扩展了回归方法的应用，使其能用来推断一个随机变量的分布函数。并且该推断还能被统计检验。作为一个应用实例。该方法被用于确定杂草种子在特定条件下扩散的分布函数。在此条件下，该杂草种子扩散为近似的高斯(正态)分布。     

基于拉格朗日模型的CO2气固两相圆管内突扩流动沉积特性分析

CO2在通过安全阀的排放过程中,形成的固体CO2在安全阀下游管道突扩处沉积,导致管路的堵塞和冻结,危害被保护系统的安全.为了寻求减少固体CO2在安全阀下游管道突扩处的沉积量和进一步避免管道堵塞的方法,利用拉格朗日模型对CO2气固两相紊流在突扩管内的流动和沉积特性进行计算,通过比较沉积率计算值与实验值,验证了计算结果的合理性;利用该模型分析了各种流动参数对流动和沉积特性的影响.结果表明,通过安全阀开启排放过程中生成的固体颗粒直径尽可能远离颗粒回流量的峰值区域(即颗粒直径0.04~0.07 mm,St数为3.2~9.8的区域),可以降低固体CO2的沉积量,从而避免管道的堵塞和冻结.具体可以通过设计适当的安全阀开启时最小流通截面面积做到.     

Trapping methods for rangeland insects in burned and unburned sites: a comparison

Different insect trapping methods were compared weekly on recently burned and nearby unburned rangeland in the Great Basin of western Utah. Flight traps (or wing traps) collected the most specimens (total = 4,916 at burned site, 4,384 at unburned site) but represented the fewest species (no more than 11 spp./wk at either site); this method was not recommended because of difficulty in removing specimens from adhesive. Water traps amassed the next largest number of specimens (￣x ± SE/wk = 150.9 ± 75.3 at burned site, 158.0 ± 66.4 at unburned site) and the most species (￣x ± SE/wk = 21.6 ± 6.4 at burned site, 35. 1 ± 4.5 at unburned site). The weekly collections from pitfall traps between the sites were significantly different (P < .05) in number of specimens (￣x ± SE/wk = 8.1 ± 1.5 at burned site, 19.7 ± 4.8 at unburned site) and species (￣x ± SE/wk = 4.7 ± 0.6 at burned site, 9.5 ± 2.0 at unburned site). Malaise traps were the most convenient. Black Malaise traps collected more specimens (￣x ± SE/wk = 99.8 ± 19.4 at burned site, 90.6 ± 16.4 at unburned site) and species (￣x ± SE/wk = 22.9 ± 3.9 at burned site, 19.4 ± 6.5 at unburned site) than white malaise traps (specimens: ￣x ± SE/wk = 72.1 ± 22.4 at burned site, 87.1 ± 31.2 at unburned site; species: ￣x ± SE/ wk = 19.7 ± 6.6 at burned site, 16.4 ± 5.4 at unburned site), although this difference was not significant.     

Western balsam bark beetle, Dryocoetes confusus Swaine, flight periodicity in northern Utah

The flight periodicity of western balsam bark beetle ( Dryocoetes confusus Swaine) in Big Cottonwood Canyon, Utah, was studied during the summer months of 1992, 1993, and 1994. Contents of baited funnel traps were tallied by species up to 3 times weekly. Two main periods of flight activity were observed each year. The first and, generally, largest occurred in early summer soon after flight was initiated for the season. A 2nd period was observed in late summer, generally August. Timing of the 2 periods was influenced by unusually warm or cool weather in each study year. The 1st period had more males than females while the 2nd period had a majority of females. Except during periods of cool or wet weather, western balsam bark beetles were found to be active at least at minimal levels from June through September.     

Danes commemorating Darwin: apes and evolution at the 1909 anniversary

This article analyses the Danish 1909 celebrations of the centenary of Charles Darwin's birth on 12 February 1809. I argue that the 1909 meetings, lectures and publications devoted to Darwin and his theory of evolution by natural selection can be characterised by ambivalence: on the one hand, tribute to a great man of science who established a new view of nature and, on the other hand, scepticism towards the Darwinian mechanism of natural selection and the wider religious and political implications drawn from his theory. The article examines both professional and popular commemorative activities, focusing primarily on celebratory articles carried in widely circulated magazines and newspapers. I identify three types of interpretations of Darwin's ideas which I characterise as ‘radical’, ‘evangelical’ and ‘safe’ science. These different positions were closely linked to the political and cultural divisions of the periodical press. Moreover, my analysis of the popular press offers a solid basis for asserting that to most people Darwinism was associated with human evolution, primarily the relationship between man and apes, while more sophisticated discussions about the crisis of Darwinism prominent among naturalists played only a secondary role in the public arena. This article demonstrates the value of using newspapers as historical sources when looking for public images of Darwin, popular receptions of Darwinism and representations of science in general.     

Disruption of calcitonin gene-related peptide signaling accelerates muscle denervation and dampens cytotoxic neuroinflammation in SOD1 mutant mice

Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease. Neuronal vacuolization and glial activation are pathologic hallmarks in the superoxide dismutase 1 (SOD1) mouse model of ALS. Previously, we found the neuropeptide calcitonin gene-related peptide (CGRP) associated with vacuolization and astrogliosis in the spinal cord of these mice. We now show that CGRP abundance positively correlated with the severity of astrogliosis, but not vacuolization, in several motor and non-motor areas throughout the brain. SOD1 mice harboring a genetic depletion of the βCGRP isoform showed reduced CGRP immunoreactivity associated with vacuolization, while motor functions, body weight, survival, and astrogliosis were not altered. When CGRP signaling was completely disrupted through genetic depletion of the CGRP receptor component, receptor activity-modifying protein 1 (RAMP1), hind limb muscle denervation, and loss of muscle performance were accelerated, while body weight and survival were not affected. Dampened neuroinflammation, i.e., reduced levels of astrogliosis in the brain stem already in the pre-symptomatic disease stage, and reduced microgliosis and lymphocyte infiltrations during the late disease phase were additional neuropathology features in these mice. On the molecular level, mRNA expression levels of brain-derived neurotrophic factor (BDNF) and those of the anti-inflammatory cytokine interleukin 6 (IL-6) were elevated, while those of several pro-inflammatory cytokines found reduced in the brain stem of RAMP1-deficient SOD1 mice at disease end stage. Our results thus identify an important, possibly dual role of CGRP in ALS pathogenesis.