Coulomb-blockade transport in single-crystal organic thin-film transistors

Coulomb-blockade transport--whereby the Coulomb interaction between electrons can prohibit their transport around a circuit--occurs in systems in which both the tunnel resistance, Rb between neighbouring sites is large (>h/e2) and the charging energy, E(C) (E(C) = e2/2C, where C is the capacitance of the site), of an excess electron on a site is large compared to kT. (Here e is the charge of an electron, k is Boltzmann's constant, and h is Planck's constant.) The nature of the individual sites--metallic, superconducting, semiconducting or quantum dot--is to first order irrelevant for this phenomenon to be observed. Coulomb blockade has also been observed in two-dimensional arrays of normal-metal tunnel junctions, but the relatively large capacitances of these micrometre-sized metal islands results in a small charging energy, and so the effect can be seen only at extremely low temperatures. Here we demonstrate that organic thin-film transistors based on highly ordered molecular materials can, to first order, also be considered as an array of sites separated by tunnel resistances. And as a result of the sub-nanometre sizes of the sites (the individual molecules), and hence their small capacitances, the charging energy dominates at room temperature. Conductivity measurements as a function of both gate bias and temperature reveal the presence of thermally activated transport, consistent with the conventional model of Coulomb blockade.     

High K+ content explains the abolition of the action potential in amphibian sciatic nerve in vitro byLathyrus sativus seed extract

Summary The action potential in amphibian sciatic nerve in vitro has been reported to be abolished by the topical application ofLathyrus sativus seed extract. We have confirmed this effect, but find that it is probably caused by the high K⁺ content of such seed extracts and that organic neurotoxins are not implicated.Acknowledgments. We thank the Wellcome Trust for financial support, Professor M.W. Bradbury for help with the ion determinations and Miss M.B. Burbridge of the Tropical Products Institute, London, for obtaining seed from Bombay, India.     

High K+ content explains the abolition of the action potential in amphibian sciatic nerve in vitro by Lathyrus sativus seed extract.

The action potential in amphibian sciatic nerve in vitro has been reported to be abolished by the topical application of Lathyrus sativus seed extract. We have confirmed this effect, but find that it is probably caused by the high K+ content of such seed extracts and that organic neurotoxins are not implicated.     

Measurement of the intracellular free calcium concentration in salamander rods

Measurement of the free calcium concentration within a photo-receptor outer segment has been considered an important aim since the proposal by Hagins and Yoshikami that the primary event in phototransduction is a release of Ca (2+) inside the cell. More recent evidence has cast doubt on the calcium hypothesis, and the observations of Yau and Nakatani and Matthews et al. suggest that the internal Ca (2+) concentration ([Ca (2+)]i), may decrease after a flash of light. In the present study we have measured [Ca (2+)]i directly by using a new method for incorporating the Ca-sensitive photoprotein aequorin into an isolated rod. We report that the light response is accompanied by a decrease in [Ca (2+)]i, caused by the closure of light-sensitive channels which are the main route for Ca (2+) entry into the outer segment. Of the Ca (2+) entering through light-sensitive channels, about 95% is sequestered by a rapid and reversible buffering mechanism. Calcium is removed from the cell by an electrogenic pump in which 3 Na (+) ions are exchanged for each Ca (2+); the pump is highly active and the free Ca (2+) in the cell declines with a time constant of ~0.5 s after a flash of light.     

Sex differences in level of a vasoactive plasma protein and changes during pregnancy

Zusammenfassung Menschenplasma enthält ein Eiweiss, welches sämtliche Blutgefässe in vitro einengt, jedoch in vivo Arterienerweiterung zu veranlassen scheint. Das Plasmaniveau von Frauen ist höher als das von Männern. Während der Schwangerschaft fällt es ab und kehrt postpartum zu dem Ausgangspunkt der Kontrollen zurück, oder es steigt sogar über diesen Ausgangspunkt hinaus.     

A relationship between the yeast cell cycle genes CDC4 and CDC36 and the ets sequence of oncogenic virus E26

We report here significant primary sequence homology among the predicted translational products of three genes: CDC4 , CDC36 and ets. CDC4 and CDC36 are Saccharomyces cerevisiae cell division cycle genes, while ets is a transformation-specific sequence of avian erythroblastosis virus E26. The deduced primary structures of the three gene products were compared by computer to a large data base of known and predicted protein sequences. The search revealed 22.0-25.5% identity over regions of 140-206 codons, respectively between the different pairwise combinations. For these particular sequences, these identity scores fall 3.4-4.0 standard deviations above the empirically-determined mean values of fortuitous similarity. S. cerevisiae calls require CDC36 and CDC4 in order to complete two early events in the cell cycle: execution of start ( CDC36 ) and spindle pole body separation ( CDC4 ). In virus E26, the ets sequence is linked in frame with delta gag and mybE in the tripartite structure 5'-delta gag- mybE -ets-3', comprising the E26 transforming oncogene. The homologies described here suggest that the biochemical functions or regulation of the CDC4 , CDC36 and ets products may be related.