翻译控制肿瘤蛋白(TCTP)研究进展

翻译控制肿瘤蛋白（The translationally controlled tumor protein,TCTP）最初发现于肿瘤组织,后来又从其他组织中相继分离出来。TCTP是一类广泛存在于动物、植物及酵母中的、在序列上高度保守且具有很高表达量的蛋白家族。最初认为TCTP是一类生长相关蛋白,近年来研究表明,代TP具有多种生物学功能。     

非下采样Contourlet HMT模型

非下采样Contourlet变换（NSCT）不仅具有类似传统小波变换和Contourlet变换的多尺度和多方向特性,而且还具有良好的平移不变特性．文中对NSCT变换系数进行研究,获得NSCT变换的方向子带系数同样具有持续性和聚集性的结论;在此基础上结合隐Markov树模型,建立了一种新的基于NSCT域的隐Markov树模型NSCT—HMT,并且给出了该模型的参数结构以及模型参数的训练方法;最后将所提出的模型应用于图像去噪中取得了很好的仿真效果．     

Density functional theory study on the insertion reaction mechanism of dibromocarbene with formaldehyde

The insertion reaction mechanism of CBr2 with CH3CH2O has been studied by using the B3LYP/6-311G(d) and CCSD(T)/6-311G(d) at single point. The geometries of reactions,transition state and products were completely optimized. All the transition state is verified by the vibrational analysis and the internal re-action coordinate (IRC) calculations. The results show that reaction (1) is the dominant reaction path,which proceeds via two steps: i) two reactants form an intermediate (IM1),which is an exothermal re-action of 8.62 kJ·mol?1 without energy barrier; ii) P1 is obtained via the TS1 and the H-shift,in which the energy barrier is 44.53 kJ·mol?1. The statistical thermodynamics and Eyring transition state theory with Wigner correction are used to study the thermodynamic and kinetic characters of this reaction in temperature range from 100 to 2200 K. The results show that the appropriate reaction temperature ranges from 200 to 1900 K at 1.0 atm,in which the reaction has a bigger spontaneity capability,equi-librium constant (K) and higher rate constant (k).     

我国无底柱分段崩落法的发展方向

我国无底柱矿山长期以来,基本上采用10m×10m(分段高度×进路间距)左右的小结构参数,限制了无底柱采矿法优越性的发挥,大间距结构参数较小结构参数而言,对增大开采强度,减少采准工程量,降低采矿成本等均具有明显的效果。大间距无底柱采矿工艺更加适合我国当前的生产力发展水平,便于应用和推广。大间距结构为我国无底柱矿山首选结构参数     

多钉连接复合材料板钉传载荷的估算

多钉连接的钉传载荷和板孔应力的计算是很重要也很复杂的一个问题。采用各向异性板的复变函数理论和边界配置方法,对连接件进行分区,加以限制条件,对多钉连接的复合材料层合板的钉载和板与螺栓间的接触应力和接触区域进行了计算。板和钉之间的接触应力显著受到连接板的几何尺寸、加载的条件、材料的各向异性、钉与孔之间的间隙、钉的柔度等因素的影响。在计算条件中施加了这些因素的影响。     

物联网技术及其在军事领域中的应用

物联网技术涵盖了当前所有先进信息技术在各个层次的部署和应用,其军事上的应用主要体现在让未来战场"更加透彻的感知"、"更加深入的智能化"和"更加精细化的保障"。文中介绍了物联网概念和相关技术,结合物联网技术在识别、定位、跟踪、监测等方面实现智能化管理和控制的用途,阐述了其在军事领域中的几种典型应用。     

Endogenously generated amyloid β increases membrane fluidity in neural 2a cells

The effect of endogenously generated amyloid β on membrane fluidity was investigated in Neural 2a cells stably expressing Swedish mutant amyloid precursor protein (APPswe). Membrane fluidity was studied by fluorescence polarizability using 1,6-Diphenyl-1,3,5-Hexatriene (DPH) as the fluorescence probe. It was found that the membrane fluidity in APPswe cells was significantly higher than that in its wild type counterparts. Alleviating the effect of amyloid β either by γ secretase activity inhibition or by amy...     

代谢综合征药物高通量筛选模型的建立

线粒体对于真核细胞至关重要, 除了为细胞提供能量, 还参与细胞信号转导、分化与生长、凋亡等生命过程. 许多疾病的发生与线粒体功能失常密切相关, 包括神经退行性疾病、糖尿病、肥胖、肿瘤等. 由于线粒体膜电位是反映细胞功能状态的重要指针, 因此选用了代谢性细胞L6大鼠肌管细胞建立了一种快速且高效地分析线粒体膜电位的高通量筛选模型. 通过对线粒体染料JC-1浓度及孵育时间、待筛选化合物孵育时间、阳性化合物CCCP(carbonylcyanide-m-chlorophenylhydrazone) 浓度等实验条件的优化, 确立了筛选条件, 并对鱼藤酮、丙二酸、抗霉素A、寡霉素和黄连素(berberine)等线粒体抑制剂进行验证, 证实该筛选体系的可靠性. 以10 μmol L^-1 CCCP作为阳性对照, 筛选体系的整体CV值(变异系数)为5.92%, Z'因子为0.575, 符合高通量筛选的要求. L6肌管细胞线粒体膜电位高通量筛选模型的建立, 将为发现治疗代谢综合征的新药先导化合物提供更多机会.     

论矩阵的方根

对矩阵开方运算进行探讨,给出了方阵存在ｎ次方根的充要条件,求ｎ次方根方法．     

Spin-orbit coupling and zero-field splitting of the high-spin ferric enzyme-substrate complex: Protocatechuate 3,4-dioxygenase complexed with 3,4-dihydroxyphenylacetate

We used density functional calculations to investigate the electronic origins of the magnetic properties of the high-spin ferric enzyme-substrate complex protocatechuate 3,4-dioxygenase(3,4-PCD).The calculated g-tensors show that ligand-to-metal charge transfer transitions are from the protocatechuate(PCA) and Tyr408 orbitals to the Fe d orbitals,which lead to x-and y-polarized transitions.These polarized transitions require a spin-orbit coupling(SOC) matrix element in the z-direction,Lz(z=z’),resulting in a g z value of 2.0158,significantly deviating from 2.0023.A large zero-field splitting parameter value of+1.147cm-1 is due to △S =-1 spin-orbit mixing with the quartet states for the sextet ground state,accounting for around 73% of the SOC contribution.The SOC matrix elements indicate that the high-spin d 5 system Fe(Ⅲ),3,4-PCD-PCA is a weak spin-crossover compound with an SOC of 31.56 cm-1.