Lysozyme refolding at high concentration by dilution and size—exclusion chromatography

This study of renaturation by dilution and size exclusion chromatography (SEC) addition of urea to improve yield as well as the initial and final protein concentrations showed that although urea decreased the rate of lysozyme refolding, it could suppress protein aggregation to sustain the pathway of correct refolding at high protein concentration; and that there existed an optimum urea concentration in renaturation buffer. Under the above conditions, lysozyme was successfully refolded from initial concentration of up to 40 mg/mL by dilution and 100 mg/mL by SEC, with the yield of the former being more than 40% and that of the latter being 34.8%. Especially, under the condition of 30 min interval time, i.e. tau > 2(t(R2) - t(R1)), the efficiency was increased by 25% and the renaturation buffer could be recycled for SEC refolding in continuous operation of downstream process.     

大开孔三段加热中温黑体炉的研制

报道了所研制的大开孔三段加热中温黑体炉腔体热源布置原则。利用靶后腔体控制靶温可使黑体空腔在各个工作温度(50—1200℃)范围内保持均匀,在280mm腔体有效长度内最大分布温差在2℃以内。利用区域近似拟合迭代法计算了腔体有效发射率分布,计算结果表明,大锥角(148.28°)靶面上各点有效发射率值近似相等,其值为0.9985。