A deficiency of the homeotic complex of the beetle Tribolium

In Drosophila, the establishment of regional commitments along most of the anterior/posterior axis of the developing embryo depends on two clusters of homeotic genes: the Antennapedia complex (ANT-C) and the bithorax complex (BX-C). The red flour beetle has a single complex (HOM-C) representing the homologues of the ANT-C and BX-C in juxtaposition. Beetles trans-heterozygous for two particular HOM-C mutations spontaneously generate a large deficiency, presumably by an exchange within the common region of two overlapping inversions. Genetic and molecular results indicate that this deficiency spans at least the interval between the Deformed and abdominal-A homologues. In deficiency homozygous embryos, all gnathal, thoracic and abdominal segments develop antennal appendages, suggesting that a gene(s) has been deleted that acts to distinguish trunk from head. There is no evidence that beetles have a homologue of the segmentation gene fushi tarazu of similar genomic location and function. On the basis of the genetic tractability, convenient genome size and organization of Tribolium, and its relatively long phylogenetic divergence from Drosophila (>300 million years), we have integrated developmental genetic and molecular analyses of the HOM-C. We isolated about 70 mutations in the complex representing at least six complementation groups. The homeotic phenotypes of adults and lethal embryos lead us to believe that these beetle genes are homologous with the Drosophila genes indicated in Fig. 1 (see text).     

苯酚-硫酸法测定藏木香中多糖含量

采用传统的水煮醇沉法从藏木香中提取可溶性多糖,通过Sevag法脱蛋白后,以葡萄糖为对照品,使用苯酚-硫酸法测定多糖含量.结果表明,在波长486nm处测定吸光度,10-100μg/mL范围内吸光度与被测含量之间具有良好的线性关系,藏木香中多糖的含量为64.37%.     

甘青青兰挥发性成分GC/MS分析

目的：分离鉴定出甘青青兰（Dracocephalum tanguticum Maxim.）挥发油的化学成分.方法：用气相色谱-质谱（GC/MS）联用技术及峰面积归一化法测定各组分的相对含量.结果：共鉴定出23种化合物,占总色谱峰总面积的87.46%.结论：甘青青兰挥发油中的化学成分主要为[-]-反-松香芹乙酯和桉油精,两者分别占总挥发油中化学成分的60.30%和9.31%.     

钢管相贯口变参数变结构建库与数据提取方法研究

零件的批处理和零件库的建立是计算机辅助设计的重要内容之一，以钢管相贯口为例应用UG的几种建库方法，通过分析电子表格、零件族、GRIP编程在变参变结构的复杂零件建库时存在的问题，提出了一种将零件族和GRIP编程相结合的建库方法来建立变参变结构的复杂零件的零件库。     

带有时变时滞的中立型随机系统的鲁棒镇定和H_∞控制

研究了带有时变时滞的中立型随机系统的鲁棒镇定和H∞控制问题.利用Lyapunov泛函方法和It o^公式,基于状态反馈控制器,以线性矩阵不等式(LMI)形式给出了闭环系统鲁棒镇定及H∞控制的新方法.最后,数值算例说明了该方法的有效性.     

The genome of the model beetle and pest Tribolium castaneum

Tribolium castaneum is a member of the most species-rich eukaryotic order, a powerful model organism for the study of generalized insect development, and an important pest of stored agricultural products. We describe its genome sequence here. This omnivorous beetle has evolved the ability to interact with a diverse chemical environment, as shown by large expansions in odorant and gustatory receptors, as well as P450 and other detoxification enzymes. Development in Tribolium is more representative of other insects than is Drosophila, a fact reflected in gene content and function. For example, Tribolium has retained more ancestral genes involved in cell-cell communication than Drosophila, some being expressed in the growth zone crucial for axial elongation in short-germ development. Systemic RNA interference in T. castaneum functions differently from that in Caenorhabditis elegans, but nevertheless offers similar power for the elucidation of gene function and identification of targets for selective insect control.     

论竞技健美操技术创新的概念与分类

该研究以竞技健美操技术创新为研究对象,采用文献资料法、案例分析法,对查阅的文献和案例进行综合的归纳分析,在研究竞技健美操技术创新发展现状基础上,结合其他项目技术创新的特点,对竞技健美操技术创新的概念进行了阐述;对竞技健美操技术创新进行了系统的分类.     

Ultrabithorax is required for membranous wing identity in the beetle Tribolium castaneum

The two pairs of wings that are characteristic of ancestral pterygotes (winged insects) have often undergone evolutionary modification. In the fruitfly, Drosophila melanogaster, differences between the membranous forewings and the modified hindwings (halteres) depend on the Hox gene Ultrabithorax (Ubx). The Drosophila forewings develop without Hox input, while Ubx represses genes that are important for wing development, promoting haltere identity. However, the idea that Hox input is important to the morphologically specialized wing derivatives such as halteres, and not the more ancestral wings, requires examination in other insect orders. In beetles, such as Tribolium castaneum, it is the forewings that are modified (to form elytra), while the hindwings retain a morphologically more ancestral identity. Here we show that in this beetle Ubx 'de-specializes' the hindwings, which are transformed to elytra when the gene is knocked down. We also show evidence that elytra result from a Hox-free state, despite their diverged morphology. Ubx function in the hindwing seems necessary for a change in the expression of spalt, iroquois and achaete-scute homologues from elytron-like to more typical wing-like patterns. This counteracting effect of Ubx in beetle hindwings represents a previously unknown mode of wing diversification in insects.