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An RNA map predicting Nova-dependent splicing regulation 总被引:1,自引:0,他引:1
Ule J Stefani G Mele A Ruggiu M Wang X Taneri B Gaasterland T Blencowe BJ Darnell RB 《Nature》2006,444(7119):580-586
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DRB-induced premature termination of late adenovirus transcription 总被引:18,自引:0,他引:18
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本文证明了公式β(n)=σ(n-1)+1其中α(n-1)是n-1次多项式微分系统的不为直线的最多条数,βn)是n次多项式微分系统的不变直线的不同斜率的最大个数。这里假设所讨论的多项式系统只有限条不变直线。 相似文献
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Nova regulates brain-specific splicing to shape the synapse 总被引:2,自引:0,他引:2
Ule J Ule A Spencer J Williams A Hu JS Cline M Wang H Clark T Fraser C Ruggiu M Zeeberg BR Kane D Weinstein JN Blume J Darnell RB 《Nature genetics》2005,37(8):844-852
Alternative RNA splicing greatly increases proteome diversity and may thereby contribute to tissue-specific functions. We carried out genome-wide quantitative analysis of alternative splicing using a custom Affymetrix microarray to assess the role of the neuronal splicing factor Nova in the brain. We used a stringent algorithm to identify 591 exons that were differentially spliced in the brain relative to immune tissues, and 6.6% of these showed major splicing defects in the neocortex of Nova2-/- mice. We tested 49 exons with the largest predicted Nova-dependent splicing changes and validated all 49 by RT-PCR. We analyzed the encoded proteins and found that all those with defined brain functions acted in the synapse (34 of 40, including neurotransmitter receptors, cation channels, adhesion and scaffold proteins) or in axon guidance (8 of 40). Moreover, of the 35 proteins with known interaction partners, 74% (26) interact with each other. Validating a large set of Nova RNA targets has led us to identify a multi-tiered network in which Nova regulates the exon content of RNAs encoding proteins that interact in the synapse. 相似文献
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Licatalosi DD Mele A Fak JJ Ule J Kayikci M Chi SW Clark TA Schweitzer AC Blume JE Wang X Darnell JC Darnell RB 《Nature》2008,456(7221):464-469
Protein-RNA interactions have critical roles in all aspects of gene expression. However, applying biochemical methods to understand such interactions in living tissues has been challenging. Here we develop a genome-wide means of mapping protein-RNA binding sites in vivo, by high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP). HITS-CLIP analysis of the neuron-specific splicing factor Nova revealed extremely reproducible RNA-binding maps in multiple mouse brains. These maps provide genome-wide in vivo biochemical footprints confirming the previous prediction that the position of Nova binding determines the outcome of alternative splicing; moreover, they are sufficiently powerful to predict Nova action de novo. HITS-CLIP revealed a large number of Nova-RNA interactions in 3' untranslated regions, leading to the discovery that Nova regulates alternative polyadenylation in the brain. HITS-CLIP, therefore, provides a robust, unbiased means to identify functional protein-RNA interactions in vivo. 相似文献
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