Absence of cooperative haemoglobin-oxygen binding in Sphenodon, a reptilian relict from the Triassic

It is generally accepted that the sigmoidal nature of the haemoglobin-oxygen dissociation curve (ODC) is necessary for efficient oxygen transport in terrestrial vertebrates because it allows large volumes of oxygen to be bound or released for relatively small changes in the partial pressure of oxygen (PO2) in the blood. Furthermore, the amount of oxygen to tissues is increased by hydrogen ions produced from the dissociation of carbon dioxide in solution. The generality of these key features of cooperative oxygen binding and the Bohr effect holds for reptiles, birds and mammals, including representatives with special respiratory requirements for diving, burrowing and living at high altitude. Sphenodon punctatus is the sole surviving representative of the ancient order of 'beakhead' reptiles (order Rhynchocephalia) which were once widely distributed during the Triassic period before the spectacular radiation of dinosaur faunas. We have now investigated the oxygen transporting properties of blood from Sphenodon and find that the ODC is hyperbolic, with a high affinity for oxygen and very small Bohr effect. This combination of characteristics is unique among terrestrial vertebrates and accords with a low demand for oxygen and limited scope for aerobic activity.     

Abnormal display of PfEMP-1 on erythrocytes carrying haemoglobin C may protect against malaria

Haemoglobin C, which carries a glutamate-to-lysine mutation in the beta-globin chain, protects West African children against Plasmodium falciparum malaria. Mechanisms of protection are not established for the heterozygous (haemoglobin AC) or homozygous (haemoglobin CC) states. Here we report a marked effect of haemoglobin C on the cell-surface properties of P. falciparum-infected erythrocytes involved in pathogenesis. Relative to parasite-infected normal erythrocytes (haemoglobin AA), parasitized AC and CC erythrocytes show reduced adhesion to endothelial monolayers expressing CD36 and intercellular adhesion molecule-1 (ICAM-1). They also show impaired rosetting interactions with non-parasitized erythrocytes, and reduced agglutination in the presence of pooled sera from malaria-immune adults. Abnormal cell-surface display of the main variable cytoadherence ligand, PfEMP-1 (P. falciparum erythrocyte membrane protein-1), correlates with these findings. The abnormalities in PfEMP-1 display are associated with markers of erythrocyte senescence, and are greater in CC than in AC erythrocytes. Haemoglobin C might protect against malaria by reducing PfEMP-1-mediated adherence of parasitized erythrocytes, thereby mitigating the effects of their sequestration in the microvasculature.     

CO and H(3)(+) in the protoplanetary disk around the star HD141569

Massive planets have now been found orbiting about 80 stars. A long outstanding question critical to theories of planet formation has been the timescale on which gas-giant planets form; in particular, stars more massive than the Sun may blow away the surrounding gas associated with their formation more quickly than it can be accumulated by the protoplanetary cores. Evidence for a protoplanet around a Herbig AeBe star (such stars are 2 3 times more massive than the Sun) would constrain the timescale of planet formation. Here we report the detection of CO and H(3)(+) emission from the 5-10-million-year-old Herbig AeBe star HD141569. We interpret the CO data as indicating that the inner disk surrounding the star is past the early phase of accretion and planetesimal formation, and that most of the gas has been cleared out to a distance of more than 17 astronomical units. CO effectively destroys H(3)(+) (ref. 2), so their presence in the same source is surprising. Moreover, H(3)(+) line emission has previously been detected only from the atmospheres of the giant planets in the Solar System. The H(3)(+) and CO may therefore be distributed in the disk at different circumstellar distances, or, alternatively, H(3)(+) may be located in the extended envelope of a protoplanet.