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DNA fingerprinting transforms the art of cell authentication. 总被引:4,自引:0,他引:4
The increasing diversity of new cell cultures is seriously stretching the capabilities of traditional methods of identification. DNA fingerprinting is set to play an important role in increasing confidence in the authenticity of cultures in research and industry. 相似文献
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A comparison has been made between the separation of nucleated cells from human bone marrow aspirates by high mol. wt polymers and the buffy coat techniques. 相似文献
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Summary Intestinal microorganisms are able to effect the metabolic reductive fission of hydrazines but not hydrazides during incubation in vitro.We thank the Medical Research Council for a studentship (to GCB). 相似文献
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Abazov VM Abbott B Abdesselam A Abolins M Abramov V Acharya BS Adams DL Adams M Ahmed SN Alexeev GD Alton A Alves GA Arnoud Y Avila C Babintsev VV Babukhadia L Bacon TC Baden A Baffioni S Baldin B Balm PW Banerjee S Barberis E Baringer P Barreto J Bartlett JF Bassler U Bauer D Bean A Beaudette F Begel M Belyaev A Beri SB Bernardi G Bertram I Besson A Beuselinck R Bezzubov VA Bhat PC Bhatnagar V Bhattacharjee M Blazey G Blekman F Blessing S Boehnlein A Bojko NI Bolton TA Borcherding F Bos K 《Nature》2004,429(6992):638-642
The standard model of particle physics contains parameters--such as particle masses--whose origins are still unknown and which cannot be predicted, but whose values are constrained through their interactions. In particular, the masses of the top quark (M(t)) and W boson (M(W)) constrain the mass of the long-hypothesized, but thus far not observed, Higgs boson. A precise measurement of M(t) can therefore indicate where to look for the Higgs, and indeed whether the hypothesis of a standard model Higgs is consistent with experimental data. As top quarks are produced in pairs and decay in only about 10(-24) s into various final states, reconstructing their masses from their decay products is very challenging. Here we report a technique that extracts more information from each top-quark event and yields a greatly improved precision (of +/- 5.3 GeV/c2) when compared to previous measurements. When our new result is combined with our published measurement in a complementary decay mode and with the only other measurements available, the new world average for M(t) becomes 178.0 +/- 4.3 GeV/c2. As a result, the most likely Higgs mass increases from the experimentally excluded value of 96 to 117 GeV/c2, which is beyond current experimental sensitivity. The upper limit on the Higgs mass at the 95% confidence level is raised from 219 to 251 GeV/c2. 相似文献
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An in vitro demonstration of proteolysis by macrophages and its increase with coumarin 总被引:1,自引:0,他引:1
Zusammenfassung Es wird gezeigt, dass die normale proteolytische Tätigkeit von stimulierten peritonealen Makrophagen der Maus durch das Benzo-pyron-Cumarin in vitro beträchtlich erhöht wird.
We would like to thank Schaper and Brümmer KG, West Germany, for the coumarin, and the Australian Research Grants Committee for their support. 相似文献
We would like to thank Schaper and Brümmer KG, West Germany, for the coumarin, and the Australian Research Grants Committee for their support. 相似文献
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S. Harvey C. G. Scanes A. Chadwick N. J. Bolton 《Cellular and molecular life sciences : CMLS》1979,35(5):694-695
Summary The effect of an acid extract of chicken hypothalami on the in vitro secretion of prolactin and growth hormone (GH) by dispersed chicken pituitary cells has been investigated. Both prolactin and GH release were stimulated in a dose related manner in the presence of the hypothalamic extract (HE). Somatostatin had no effect on the basal or HE stimulated release of prolactin although it did inhibit the HE induced release of GH. 相似文献
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Sexton PF Norris RD Wilson PA Pälike H Westerhold T Röhl U Bolton CT Gibbs S 《Nature》2011,471(7338):349-352
'Hyperthermals' are intervals of rapid, pronounced global warming known from six episodes within the Palaeocene and Eocene epochs (~65-34 million years (Myr) ago). The most extreme hyperthermal was the ~170 thousand year (kyr) interval of 5-7 °C global warming during the Palaeocene-Eocene Thermal Maximum (PETM, 56?Myr ago). The PETM is widely attributed to massive release of greenhouse gases from buried sedimentary carbon reservoirs, and other, comparatively modest, hyperthermals have also been linked to the release of sedimentary carbon. Here we show, using new 2.4-Myr-long Eocene deep ocean records, that the comparatively modest hyperthermals are much more numerous than previously documented, paced by the eccentricity of Earth's orbit and have shorter durations (~40?kyr) and more rapid recovery phases than the PETM. These findings point to the operation of fundamentally different forcing and feedback mechanisms than for the PETM, involving redistribution of carbon among Earth's readily exchangeable surface reservoirs rather than carbon exhumation from, and subsequent burial back into, the sedimentary reservoir. Specifically, we interpret our records to indicate repeated, large-scale releases of dissolved organic carbon (at least 1,600 gigatonnes) from the ocean by ventilation (strengthened oxidation) of the ocean interior. The rapid recovery of the carbon cycle following each Eocene hyperthermal strongly suggests that carbon was re-sequestered by the ocean, rather than the much slower process of silicate rock weathering proposed for the PETM. Our findings suggest that these pronounced climate warming events were driven not by repeated releases of carbon from buried sedimentary sources, but, rather, by patterns of surficial carbon redistribution familiar from younger intervals of Earth history. 相似文献
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T. B. Bolton R. J. Lang T. Takewaki C. D. Benham 《Cellular and molecular life sciences : CMLS》1985,41(7):887-894
Summary Dispersal of the constituent cells of mammalian visceral and vascular smooth muscles has permitted recordings both of membrane currents under whole-cell voltage clamp, and of currents through single ionic channels using the patch-clamp technique. A rectangular depolarizing step applied to a single cell under voltage clamp yielded a net inward current followed by a net outward current in normal physiological solution. In isolated, inside-out patches of cell membrane a calcium- and potential-sensitive K channel (100 pS conductance) and a calcium-insensitive, potential-sensitive K+ channel (50 pS conductance) with slow kinetics have so far been identified and characterized. 相似文献