Two classes of metabolites fromTheonella swinhoei are localized in distinct populations of bacterial symbionts

The marine spongeTheonella swinhoei (lithistid Family Theonellidae, Order Astrophorida) has yielded many important, bioactive natural products, most of which share structural features with bacterial natural products. The presence of microbial symbionts inT. swinhoei has been reported, and it was originally suggested that the cytotoxic macrolide swinholide A and many of the bioactive cyclic peptides fromT. swinhoei were all produced by symbiotic cyanobacteria. By transmission electron microscopy, we found four distinct cell populations to be consistently present inT. swinhoei: eukaryotic sponge cells, unicellular heterotrophic bacteria, unicellular cyanobacteria and filamentous heterotrophic bacteria. Purification and chemical analyses of each cell type showed the macrolide swinholide A to be limited to the mixed population of unicellular heterotrophic bacteria, and an anti-fungal cyclic peptide occurred only in the filamentous heterotrophic bacteria. Contrary to prior speculation, no major metabolites were located in the cyanobacteria or sponge cells.     

A hybrid forecasting approach for piece‐wise stationary time series

We consider the problem of forecasting a stationary time series when there is an unknown mean break close to the forecast origin. Based on the intercept‐correction methods suggested by Clements and Hendry (1998) and Bewley (2003), a hybrid approach is introduced, where the break and break point are treated in a Bayesian fashion. The hyperparameters of the priors are determined by maximizing the marginal density of the data. The distributions of the proposed forecasts are derived. Different intercept‐correction methods are compared using simulation experiments. Our hybrid approach compares favorably with both the uncorrected and the intercept‐corrected forecasts. Copyright © 2006 John Wiley & Sons, Ltd.     

Sequence comparison of human pituitary growth hormone,human chorionic somatomammotropin and ovine pituitary lactogenic hormone

Zusammenfassung Ein Vergleich der Aminosäuresequenzen von menschlichen Wachstumshormonen und menschlichem Chorion-Somatomammotropin mit denjenigen von Prolactin zeigt, dass 26% des Rückstandes in allen drei Molekülen die gleiche Position hatten mit zusätzlichen 34% als möglichen Ersatz. Alle drei Hormone scheinen einen gleichen Ursprung zu haben.     

Superfluid helium: visualization of quantized vortices

When liquid helium is cooled to below its phase transition at 2.172 K, vortices appear with cores that are only ?ngstr?ms in diameter, about which the fluid circulates with quantized angular momentum. Here we generate small particles of solid hydrogen that can be used to image the cores of quantized vortices in their three-dimensional environment of liquid helium. This technique enables the geometry and interactions of these vortices to be observed directly.     

Field-tuned quantum effects in a triangular-lattice Ising magnet

We report thermodynamic and neutron scattering measurements of the triangular-lattice quantum Ising magnet TmMgGaO4 in longitudinal magnetic fields.Our experime...     

Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9

Variable regions 1 and 2 (V1/V2) of human immunodeficiency virus-1 (HIV-1) gp120 envelope glycoprotein are critical for viral evasion of antibody neutralization, and are themselves protected by extraordinary sequence diversity and N-linked glycosylation. Human antibodies such as PG9 nonetheless engage V1/V2 and neutralize 80% of HIV-1 isolates. Here we report the structure of V1/V2 in complex with PG9. V1/V2 forms a four-stranded β-sheet domain, in which sequence diversity and glycosylation are largely segregated to strand-connecting loops. PG9 recognition involves electrostatic, sequence-independent and glycan interactions: the latter account for over half the interactive surface but are of sufficiently weak affinity to avoid autoreactivity. The structures of V1/V2-directed antibodies CH04 and PGT145 indicate that they share a common mode of glycan penetration by extended anionic loops. In addition to structurally defining V1/V2, the results thus identify a paradigm of antibody recognition for highly glycosylated antigens, which-with PG9-involves a site of vulnerability comprising just two glycans and a strand.     

A forecasting comparison of classical and Bayesian methods for modelling logistic diffusion

A Bayesian procedure for forecasting S‐shaped growth is introduced and compared to classical methods of estimation and prediction using three variants of the logistic functional form and annual times series of the diffusion of music compact discs in twelve countries. The Bayesian procedure was found not only to improve forecast accuracy, using the medians of the predictive densities as point forecasts, but also to produce intervals with a width and asymmetry more in accord with the outcomes than intervals from the classical alternative. While the analysis in this paper focuses on logistic growth, the problem is set up so that the methods are transportable to other characterizations of the growth process. Copyright © 2001 John Wiley & Sons, Ltd.