Fiber optic biosensor of immobilized firefly luciferase

Luciferase from firefly lantern extract was immobilized on CNBr-activated Sepharose 4B,The kinetic properties of immobilized luciferase were extensively studied.The Km‘ for D-luciferin is 11.9umol/L,the optimum pH and temperature for Sepharose-bound enzyme were 7.8 and 25℃ respectively.A luminescence fiber optic biosensor,making use of immobilized crude luciferase was developed for assay of ATP.The peak light intensity was linear with respect to ATP concentration in range of 10^-9-10^-5mol/L.A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.     

等离子体技术与生物材料的表面改性

简述了等离子体聚合和表面处理技术特性，并对近年来，采用等离子体技术改善生物材料表面性质的研究和应用状况作了综述．