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排序方式: 共有255条查询结果,搜索用时 171 毫秒
1.
HE Chenxia FENG Dengmin WU Wenjun DING Youfa CHEN Li CHEN Haoming YAO Jihua SHEN Qi LU Daru & XUE Jinglun . State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai China . Changhai Hospital The Second Military Medical University Shang-hai China . Hospital of Lishui Zhejiang China Correspondence should be addressed to Xue Jinglun 《科学通报(英文版)》2003,48(8)
The transfection of plasmid DNA into mammalian cells is an indispensable tool in the study of gene transfer and gene function. Since the original report in 1990 on the successful expression of a reporter gene in muscle[1], plasmid DNA injection has been widely used to mediate gene transfer study. As a gene transfer vector, naked DNA has many obvious advantages. It is easy and cheap to be prepared and more safe after transfection. But the plasmid mediated gene expression is low because of t… 相似文献
2.
ZHANG Xinbao D. E. Walling FENG Mingyi & WEN Anbang . Chengdu Institute of Mountain Hazards Environment Chinese Academy of Sciences 《科学通报(英文版)》2003,48(8):813-818
Throughout the past decade, the potential for using nuclear weapon-produced 137Cs ( half-life 30.2 a) fallout to quantify soil erosion rates on both cultivated and uncul- tivated lands for medium-term timescales (≈ 40 a) has been successfully demonstrated in a wide range of envi- ronments in different regions of the world[1—6]. Because in difficulties of distinguishing Chernobyl-derived compo- nent from total 137Cs input in many parts of Europe, and in measuring low activity samples of… 相似文献
3.
LU Anhuai GAO Xiang QIN Shan & WANG ChangqiuDepartment of Geology Peking University Beijing . China Correspondence should be addressed to Lu Anhuai 《科学通报(英文版)》2003,48(9):920-923
The Xiangtan manganese deposit (XTM) used to be considered a supergene oxide manganese ore in South China. We reported a new identification of the naturally outcropping cryptomelane by examining the physical, chemical and structural features of the XTM supergene oxide manganese ore. The MnO2 content was over 90%, K2O more than 3%, and water from 2.2%-3.1% which is similar to one in zeolite. The cell parameters of the cryptomelane were given as a0 = 0.9974 nm, b0 = 0.2863 nm, c0 = 0.9693 nm and β= 91.47°. There was a larger pseduotetragonal tunnel in the natural cryptomelane that was formed by [MnO6] octahedral double chains with aperture of 0.462×0.466 nm2, filled with K cations resulting in some Mn3+ substituting for Mn4+ to balance the negative charges of structure. The finding is important not only for prospecting manganese resources in South China, but also in application of octahedral molecular sieve of natural cryptomelane as that developed in the tetragonal molecular sieve of natural zeolite o 相似文献
4.
Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection 总被引:6,自引:0,他引:6
SHI Rong MA Wenli WU Qinghua ZHANG Bao SONG Yanbin GUO Qiuye XIAO Weiwei WANG Yan & ZHENG Wenling . Institute of Molecular Biology First Military Medical University Guangzhou China . Department of Medical Research Guangzhou Liu Hua Qiao Hospital Guangzhou China Correspondence should be addressed to Ma Wenli 《科学通报(英文版)》2003,48(12):1165-1169
In April 2003, a novel coronavirus[1,2] which was associated with cases of Severe Acute Respiratory Syn-drome (SARS) was first isolated and sequenced in Canada. The genome of SARS coronavirus (SARS-CoV) is 29727[3] nucleotides in length and has 11 known open reading frames (ORFs). Although the genome organiza-tion of this virus is similar to that of other coronaviruses, phylogenetic analyses and sequence alignment show that SARS-CoV is not closely related to any of the previously ch… 相似文献
5.
Do multiple cycles of aeolian deposit-pedogenesis exist in the reticulate red clay sections in southern China? 总被引:4,自引:0,他引:4
HU Xuefeng CHENG Tianfan & WU Hexin Department of Environmental Science Engineering Shanghai Uni-versity Shanghai China Correspondence should be addressed to Hu Xuefeng 《科学通报(英文版)》2003,48(12):1251-1258
Since the Quaternary scientists successfully dis-closed the paleoclimatic significance of the loess-paleo- sol sequence in the Loess Plateau, Northwest China, the loess-paleosol sequence has become well-known terres-trial materials revealing the multiple cyclic changes of Quaternary climates. As a carrier of paleoenvironmental information, it could compare beauty with the deep ocean deposit[8]. In recent years, however, the researchers began to put their sights to southern China. They try t… 相似文献
6.
Climatic significance of stable carbon isotope in tree rings of Abies spectabibis in southeastern Tibet 总被引:3,自引:0,他引:3
LIU Xiaohong Qin Dahe SHAO Xuemei CHEN Tuo & REN Jiawen . Cold Arid Regions Environment Engineering Research Insti-tute Chinese Academy of Sciences Lanzhou China . Institute of Geographic Sciences Nature Resources Research Chinese Academy of Sciences Beijing China Correspondence should be addressed to Liu Xiaohong 《科学通报(英文版)》2003,48(18):2000-2004
The annually cross-dated stable carbon isotope of tree-ring a-cellulose of Abies spectabibis collected from the southeastern Tibetan Plateau is used to examine its rela-tionship with climatic parameters. The residual D13C series in treerings is constructed after removing the effects of age trend and rising CO2. We found a close relationship between D13C in tree rings and the relative humidity of September—November of the previous year measured at the nearby Ny-ingchi Meteorological Station, albeit a strong 搇agged effect? Thus we developed a transfer function to reconstruct the autumn relative humidity for the Nyingchi region, which explained 37.9% of the total variance (p < 0.001). Our results suggest a high frequency and moderate amplitude variance of the relative humidity before 1800, and the variance re-versed afterwards. 相似文献
7.
WANG Dongxiao LIU Xiongbin WANG Wenzhi DU Yan & ZHOU Weidong LED South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou Correspondence should be addressed to Wang Dongxiao 《科学通报(英文版)》2004,49(7)
The South China Sea (SCS) is the largest marginal sea in the northwestern Pacific. Several important water gateways exist around the SCS, such as Luzon Strait, Taiwan Strait, etc., that lie in the northeastern SCS, and others like Kalimantan Strait, Balawan Strait, etc., lie in the south. Luzon Strait connects the SCS and Pacific via the Philippine Sea, and its water exchange has significant impact on the interior circulations of the SCS[1]. Since direct observation data about the … 相似文献
8.
安全管理工作的一个重要目标是避免各类事故的发生,而避免事故的前提是对事故隐患进行排查治理。事故隐患排查治理是企业落实安全第一、预防为主、综合治理安全方针的形式之一,是预防事故的重要手段。本文分析了铁路施工企业在事故隐患排查治理中存在的一般安全生产频发的问题,并提出了对策。 相似文献
9.
一望无垠的孤烟大漠托起一座座高大宏伟的黄土金字塔形建筑,在广袤的西部天空下显得格外雄壮。逝去的岁月,埋藏着帝国多少的恢宏与辛酸;失落的文明,涵盖了党项民族多少的永恒和更变。一股充满着神奇的力量,庇护着这方乐土,它的神秘面纱能否被揭开,使其重现人间?屏住呼吸,让时光倒流,带着你的疑惑,和我一起穿越到西夏王国的时空,去探寻千百年的美好宏愿。壮观而神秘的西夏王陵在宁夏回族自治区首府银川以西约30km的贺兰山麓下,西夏王陵九代帝王安息之所款款坐落于此。沉睡900多年,依然巍然挺立。 相似文献
10.
Fei Jinggao 《系统工程与电子技术(英文版)》1994,(3)
Some of Advances on the Error Analysis in the Frequency Domain for a Digital Simulation ModelFeiJinggao(BeijingInstituteofCom... 相似文献