mRNA differential display on gene expression in settlement metamorphosis process of Ruditapes philippinarum larvae

The mRNA differential display （DDRT-PCR） technique was adopted to find out the genes related to settlement metamorphosis development process of Ruditapes philippinarum larvae. In this study, we have obtained three hundred and forty-six amplification bands in total from pediveliger larvae, veliger larvae, eye spot larvae and post-larvae. Sixty-five out of three hundred and forty-six bands are distinctly differential display from band pattern, which can be put into four groups, standing for different expression characters. Sixteen differential display bands were cloned, sequenced and analyzed and nine different sequences are obtained in the study. Three sequences have higher similarity to the cDNAs deposited in database and three are very similar to the rDNA of other species, considered as the rDNA of Ruditapes philippi narum. The rest three sequences are found to be novel sequences after analyzed. Their accession numbers are AY916799, AY916798, and AY916797 respectively. We thought the novel sequences are possibly relevant to the early embryo development of Ruditapes philippinarum larvae and can provide some fundamental understandings that are helpful for the improvement of scallop seed raising industry.     

化工原理流体力学自主设计性实验的教学实践

流体力学综合实验是化工原理课程教学中一个非常重要的实验,其中涉及到离心泵特性、管路特性、流体流动阻力测定等内容,为了提高学生独立操作、独立思考的能力并培养其工程观念,我校将其扩展为自主设计性实验,由学生自己设计、组装实验装置,在此过程中,学生们认识了大量在课堂上见不到的管道、三通、弯头、阀门等工程管件;在优化工艺、独立处理实验中的问题、解决事故的过程中,学生们与实际工程问题零距离接触,大大提高了他们处理工程问题的能力,收到了很好的成效,将其作为基础资料,对今后的实验教学改革提供依据。