The knockout mouse project

Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes. Furthermore, many of these are limited in utility because they have not been made or phenotyped in standardized ways, and many are not freely available to researchers. It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain.     

喀斯特自然土壤中AM真菌对先锋植物根系的影响

【目的】研究丛枝菌根(Arbuscular mycorrhiza，AM)真菌在喀斯特自然土壤条件下对喀斯特先锋草本植物根系的影响。【方法】通过自然土接种AM真菌(N)、灭菌土接种AM真菌(M)及灭菌土壤对照(S)共3种土壤处理，种植喀斯特先锋植物狗尾草(Setaria viridis)、荩草(Arthraxon hispidus)、鬼针草(Bidens pilosa)及狼杷草(Bidens tripartita)，并测定它们的根系生物量、根长、根表面积、根体积、根平均直径、根尖数及根分枝数。【结果】荩草、鬼针草及狼杷草在N处理及M处理下具有较高的菌根侵染率，狗尾草的菌根侵染率较低。与S处理相比，M处理下AM真菌明显提高了荩草、鬼针草及狼杷草的根系生物量、根长、根表面积、根体积、根尖数、根分枝数及组织密度，降低了根平均直径、比根长、比根面积及比根体积；与M处理相比，N处理明显降低了荩草、鬼针草及狼杷草的根系生物量、根长、根表面积、根体积、根尖数、根分枝数及组织密度，提高了比根长、比根面积及比根体积，但对根平均直径无明显影响。【结论】荩草、鬼针草及狼杷草具有较高菌根侵染率，能与AM真菌共生获得更加发达的根系，而自然土壤削弱了AM真菌对荩草、鬼针草及狼杷草根系生长的促进作用。     

基于并行工程的产品开发过程管理的研究

并行工程采用集成化与并行化的思想来开发产品,强调在信息集成基础上的过程集成,运用工作流管理技术能够很好地达到此目的。在分析工作流管理技术的基础上,以冲压模具的开发过程为例来说明用工作流管理技术实现产品开发过程管理的     

Mosaic overgrowth with fibroadipose hyperplasia is caused by somatic activating mutations in PIK3CA

The phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway is critical for cellular growth and metabolism. Correspondingly, loss of function of PTEN, a negative regulator of PI3K, or activating mutations in AKT1, AKT2 or AKT3 have been found in distinct disorders featuring overgrowth or hypoglycemia. We performed exome sequencing of DNA from unaffected and affected cells from an individual with an unclassified syndrome of congenital progressive segmental overgrowth of fibrous and adipose tissue and bone and identified the cancer-associated mutation encoding p.His1047Leu in PIK3CA, the gene that encodes the p110α catalytic subunit of PI3K, only in affected cells. Sequencing of PIK3CA in ten additional individuals with overlapping syndromes identified either the p.His1047Leu alteration or a second cancer-associated alteration, p.His1047Arg, in nine cases. Affected dermal fibroblasts showed enhanced basal and epidermal growth factor (EGF)-stimulated phosphatidylinositol 3,4,5-trisphosphate (PIP(3)) generation and concomitant activation of downstream signaling relative to their unaffected counterparts. Our findings characterize a distinct overgrowth syndrome, biochemically demonstrate activation of PI3K signaling and thereby identify a rational therapeutic target.     

Thiaminpyrophosphatase bei der experimentellen Diphtherieintoxikation

Summary The determinations of thiamine pyrophosphatase activity were carried out in the liver homogenates of normal guinea-pigs and those poisoned with diphtheria toxin.The enzyme activity in the toxaemic liver tissue is twice that of normal guinea-pigs. It is supposed that this result may partly explain the increased level of pyruvic acid in the blood of patients with diphtheria. Further studies on this problem are under way.     

GH220合金中碳化物相变规律

研究了合金中M_6C,M_(23)C_6及MC的晶体结构、化学组成、形态、分布及数量,它们的溶解析出规律及互相转化。