手性药物拆分方法的研究

本通过对几种拆分手性药物的方法的列举，比较几种方法，详细介绍了几种方法的特点，以及它们的不同作用。各个方法中又列出了不同的线索，根据这些线索来研究这几种拆分的方法，它们的优缺点以及针对这些缺点的解决方法，最后给出了几个典型的用该方法的实验，以辨别它们的效用。     

Identification of the gene responsible for methylmalonic aciduria and homocystinuria, cblC type

Methylmalonic aciduria and homocystinuria, cblC type (OMIM 277400), is the most common inborn error of vitamin B(12) (cobalamin) metabolism, with about 250 known cases. Affected individuals have developmental, hematological, neurological, metabolic, ophthalmologic and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood. The cblC locus was mapped to chromosome region 1p by linkage analysis. We refined the chromosomal interval using homozygosity mapping and haplotype analyses and identified the MMACHC gene. In 204 individuals, 42 different mutations were identified, many consistent with a loss of function of the protein product. One mutation, 271dupA, accounted for 40% of all disease alleles. Transduction of wild-type MMACHC into immortalized cblC fibroblast cell lines corrected the cellular phenotype. Molecular modeling predicts that the C-terminal region of the gene product folds similarly to TonB, a bacterial protein involved in energy transduction for cobalamin uptake.     

普通化学实验教学改革探索与实践

针对多年来农业院校传统的普通化学实验课教学,进行了教学模式、教学内容、考核方式和实验管理体系等全方位的改革探索和实践.结果表明,经过实验教学改革与实践使学生的实验基本技能、综合素质能力和实验水平有了很大的提高,取得了满意的教学效果.