Energetic landscape of alpha-lytic protease optimizes longevity through kinetic stability.

During the evolution of proteins the pressure to optimize biological activity is moderated by a need for efficient folding. For most proteins, this is accomplished through spontaneous folding to a thermodynamically stable and active native state. However, in the extracellular bacterial alpha-lytic protease (alphaLP) these two processes have become decoupled. The native state of alphaLP is thermodynamically unstable, and when denatured, requires millennia (t1/2 approximately 1,800 years) to refold. Folding is made possible by an attached folding catalyst, the pro-region, which is degraded on completion of folding, leaving alphaLP trapped in its native state by a large kinetic unfolding barrier (t1/2 approximately 1.2 years). alphaLP faces two very different folding landscapes: one in the presence of the pro-region controlling folding, and one in its absence restricting unfolding. Here we demonstrate that this separation of folding and unfolding pathways has removed constraints placed on the folding of thermodynamically stable proteins, and allowed the evolution of a native state having markedly reduced dynamic fluctuations. This, in turn, has led to a significant extension of the functional lifetime of alphaLP by the optimal suppression of proteolytic sensitivity.     

O''''-Sialon-ZrO2复相材料反应烧结过程中的相组成及显微结构研究

研究了CaO稳定的氧化锆、锆英石、Si3N4及活性α-Al2O3体系反应烧结制备O＇-Sialon—ZrO2复相材料过程中物相组成的变化及显微结构特征一结果表明，1550℃下体系中已经形成了所谓的过渡液相，这种过渡液相的存在，加速了锆英石的分解，促进了O＇-Sialon“溶解-扩散-再结晶”的结晶过程。1600℃及以上温度烧后，锆英石完全分解，随着烧结温度的提高，基质中O＇-Sialon针状晶体发育长大并形成了比较紧密的、晶体间相互交织的显微结构，与此同时，由于Ca^2＋的脱溶，稳定氧化锆骨料大晶粒分解为相对细小的m-ZrO2。     

基于组态软件的隔离开关视频监控系统设计

针对现阶段组态软件中,对于视频监控的支持还远远不能达到工程的要求。本文章探讨并实现了如何在通用组态软件中很好的嵌入自主开发的视频监控模块,以实现隔离开关的视频监控和传统监控一体化功能。     

《淮南万毕术》夏造冰之新解——兼与厚宇德先生商榷

二十多年来学术界关于《淮南万毕术》“夏造冰”问题的讨论颇具戏剧性．1983年洪震襄否定了这种可能性，十年之后李志超、赵虹君的相关研究得出了相反的结果．2004年厚宇德研究了李、赵肯定说实验方案，得到出乎意料的相反结论．本文根据原文提出一个新的技术思路，按此思路，则有可能实现“夏造冰”．实际操作中能不能达到预想的目标，还有待于实验验证．     

试论发展文化产业的重要意义

当今,发展文化产业有着十分重要的意义。它不仅有助于培养新的经济增长点,扩大城市就业,实现经济社会的可持续发展,而且能够更好地满足人民群众日益增长的文化需求,推动社会主义和谐社会的建设。     

C1 inhibitor hinge region mutations produce dysfunction by different mechanisms.

Heterozygosity for a mutant dysfunctional C1 inhibitor protein, a member of the serine proteinase inhibitor (serpin) superfamily, results in type II hereditary angioneurotic oedema. We identified a "hinge" region mutation in C1 inhibitor with a Val to Glu replacement at P14 Val-432. Recombinant C1 inhibitors P10 Ala-->Thr and P14Val-->Glu did not form stable complexes with fluid phase C1s or kallikrein. The P14 Val-->Glu mutant, however, was cleaved to a 96K form by C1s, while the P10 Ala-->Thr mutant was not. The recombinant P10 mutant also did not complex with C1s, kallikrein or beta-factor Xlla-Sepharose. The two mutations, therefore, result in dysfunction by different mechanisms: in one (P14 Val-->Glu), the inhibitor is converted to a substrate, while in the other (P10 Ala-->Thr), interaction with target protease is blocked.     

微弱信号提取中的小波方法研究

针对于实际应用中的小信号特别是完全被噪声淹没情况下的微弱信号提取的问题，本文依据白噪声信号的小波变换系数相对比有用信号的小波系数小的特点，利用小波变换对信号进行消噪来提取微弱信号，仿真结果表明：小波变换能够有效的消除噪声，将有用微弱信号从受噪声污染的信号中提取出来。     

基于选区的彩色图像抽取和融合算法研究

给出了一种利用图像中定义的选择区域（选区），从复杂背景中尽可能保持原样地提取出所关心图像的图像抽取方法；同时还介绍了如何对抽出的图像进行融合设置，进行看似自然的、无缝的融合；另外对算法的应用过程进行了适当的描述，作出算法的实际使用情况说明．     

基于NS2的网络教学研究

网络仿真是研究网络协议的有效手段，同时也是向学生展示网络协议的工作状况和性能的方法。介绍网络仿真软件NS2的工作原理和特点，探讨NS2在网络教学中的应用，并给出了应用实例。     

大学英语分层教学探讨

由于高校扩招，学生入校时英语成绩两极分化十分严重，这对大学英语教学形成了挑战。因材施教的教学原则和“i＋1”理论为大学英语分层教学提供了理论依据：按学生英语水平分层教学，教师有针对性地备课施教，并通过有效的教学管理机制，使学生始终处于积极活跃的状态，以达到较好的教学效果。