首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   9篇
  免费   0篇
研究方法   2篇
综合类   7篇
  2008年   1篇
  2006年   2篇
  2005年   1篇
  1999年   1篇
  1992年   1篇
  1979年   1篇
  1978年   1篇
  1965年   1篇
排序方式: 共有9条查询结果,搜索用时 194 毫秒
1
1.
A transcriptional hierarchy involved in mammalian cell-type specification.   总被引:41,自引:0,他引:41  
  相似文献   
2.
An RNA map predicting Nova-dependent splicing regulation   总被引:1,自引:0,他引:1  
  相似文献   
3.
Kuriyan J  Darnell JE 《Nature》1999,398(6722):22-3, 25
  相似文献   
4.
DRB-induced premature termination of late adenovirus transcription   总被引:18,自引:0,他引:18  
N W Fraser  P B Sehgal  J E Darnell 《Nature》1978,272(5654):590-593
  相似文献   
5.
6.
7.
8.
Nova regulates brain-specific splicing to shape the synapse   总被引:2,自引:0,他引:2  
Alternative RNA splicing greatly increases proteome diversity and may thereby contribute to tissue-specific functions. We carried out genome-wide quantitative analysis of alternative splicing using a custom Affymetrix microarray to assess the role of the neuronal splicing factor Nova in the brain. We used a stringent algorithm to identify 591 exons that were differentially spliced in the brain relative to immune tissues, and 6.6% of these showed major splicing defects in the neocortex of Nova2-/- mice. We tested 49 exons with the largest predicted Nova-dependent splicing changes and validated all 49 by RT-PCR. We analyzed the encoded proteins and found that all those with defined brain functions acted in the synapse (34 of 40, including neurotransmitter receptors, cation channels, adhesion and scaffold proteins) or in axon guidance (8 of 40). Moreover, of the 35 proteins with known interaction partners, 74% (26) interact with each other. Validating a large set of Nova RNA targets has led us to identify a multi-tiered network in which Nova regulates the exon content of RNAs encoding proteins that interact in the synapse.  相似文献   
9.
HITS-CLIP yields genome-wide insights into brain alternative RNA processing   总被引:2,自引:0,他引:2  
Protein-RNA interactions have critical roles in all aspects of gene expression. However, applying biochemical methods to understand such interactions in living tissues has been challenging. Here we develop a genome-wide means of mapping protein-RNA binding sites in vivo, by high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP). HITS-CLIP analysis of the neuron-specific splicing factor Nova revealed extremely reproducible RNA-binding maps in multiple mouse brains. These maps provide genome-wide in vivo biochemical footprints confirming the previous prediction that the position of Nova binding determines the outcome of alternative splicing; moreover, they are sufficiently powerful to predict Nova action de novo. HITS-CLIP revealed a large number of Nova-RNA interactions in 3' untranslated regions, leading to the discovery that Nova regulates alternative polyadenylation in the brain. HITS-CLIP, therefore, provides a robust, unbiased means to identify functional protein-RNA interactions in vivo.  相似文献   
1
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号