运用试验设计法优化某军品压铸弹尾工艺

根据某军品部件出现的问题，应用试验设计法成功地找出了最优组合，提高了该部件的强度，解决了该强度问题，使生产得以正常进行。     

波纹纤维混凝土的动力耗散研究

根据曲线纤维的几何特点和受力性能 ,推导了波纹纤维混凝土的本构关系 ,在本构理论的基础上进行动力耗散研究     

管理信息系统教学刍议

企业管理的发展变化和计算机信息技术的迅猛发展,给管理信息系统的教学提出了更高的要求,要求管理信息系统的教学体系要不断完善、教学内容要不断充实,教学方式要不断改进。从预备知识的重要性、教学内容的更新、强化案例教学、加强实践环节等方面进行探讨,探索出一条真正有效、切实可行的教学改革之路。     

Structure of astacin and implications for activation of astacins and zinc-ligation of collagenases.

Astacin, a digestive zinc-endopeptidase from the crayfish Astacus astacus L., is the prototype for the 'astacin family', which includes mammalian metallo-endopeptidases and developmentally regulated proteins of man, fruitfly, frog and sea urchin. Here we report the X-ray crystal structure of astacin, which reveals a deep active-site cleft, with the zinc at its bottom ligated by three histidines, a water molecule and a more remote tyrosine. The third histidine (His 102) forms part of a consensus sequence, shared not only by the members of the astacin family, but also by otherwise sequentially unrelated proteinases, such as vertebrate collagenases. It may therefore represent the elusive 'third' zinc ligand in these enzymes. The amino terminus of astacin is buried forming an internal salt-bridge with Glu 103, adjacent to His 102. Astacin pro-forms extended at the N terminus, as observed for some 'latent' mammalian astacin homologues, did not exhibit this 'active' conformation, indicating an activation mechanism reminiscent of trypsin-like serine proteinases.     

浅谈普通话的可感性特点

作为审美对象,普通话和其它美的形象一样,是具有特殊规定性的形式和内容的统一体,从形式上看,普通话的可感形象体现在它的语音美及节奏美。     

对交通隧道防排水原则的探讨

从保护地下水环境和隧道防排水的技术经济效果两个方面，阐述了隧道工程控制型防排水的必然性和必要性，并建议了两种模型。     

Development and function of T cells in mice rendered interleukin-2 deficient by gene targeting.

Interleukin-2 (IL-2) is a lymphocytotropic hormone which is thought to have a key role in the immune response of mammalian cells. It is produced by a subpopulation of activated T-lymphocytes and acts in vitro as the principal auto- and paracrine T-cell growth factor (for reviews see refs 1-3). IL-2 is, however, not the sole T-cell growth factor, nor does it act exclusively on T cells, also promoting growth of NK cells and differentiation of B cells. A role for IL-2 in T-cell development has been postulated but remains controversial. Here we test the requirement for IL-2 in vivo using IL-2-deficient mice generated by targeted recombination. We find that mice homozygous for the IL-2 gene mutation are normal with regard to thymocyte and peripheral T-cell subset composition, but that a dysregulation of the immune system is manifested by reduced polyclonal in vitro T-cell responses and by dramatic changes in the isotype levels of serum immunoglobulins.     

A small GTP-binding protein dissociates from synaptic vesicles during exocytosis.

Low-molecular-weight GTP-binding proteins are strong candidates for regulators of membrane traffic. In yeast, mutations in the sec4 or ypt1 genes encoding small GTP-binding proteins inhibit constitutive membrane flow at the plasma membrane or Golgi complex, respectively. It has been suggested that membrane fusion-fission events are regulated by cycling of small GTP-binding proteins between a membrane-bound and free state, but although most of these small proteins are found in both soluble and tightly membrane-bound forms, there is no direct evidence to support such cycling. In rat brain a small GTP-binding protein, rab3A, is exclusively associated with synaptic vesicles, the secretory organelles of nerve terminals. Here we use isolated nerve terminals to study the fate of rab3A during synaptic vesicle exocytosis. We find that rab3A dissociates quantitatively from the vesicle membrane after Ca2(+)-dependent exocytosis and that this dissociation is partially reversible during recovery after stimulation. These results are direct evidence for an association-dissociation cycle of a small GTP-binding protein during traffic of its host membrane.     

高校图书馆“学科馆员”制度的建立与馆员继续教育

针对目前高校图书馆高贵质人才匮乏的现状，论述了高校图书馆“学科馆员”制度建立的必然性及对“学科馆员”的高贵质要求，指出馆员的继续教育是培养“学科馆员”的主要人才保障，提出了“学科馆员”继续教育的内容及方式。