Systemic praxis in the education of the agricultural systems practitioner

This paper describes the context and the systemic experiential theories that have informed the praxis of educating agricultural systems practitioners. The praxis has involved a process of action research with students and with clients in farming and other rural community organizations. The praxis encourages learners to bring a range of methodologies of inquiry to bear upon problematic issues, contingent upon the nature of such issues. Informed by a number of different theories, and by reflecting on our own work, an earlier model of a nested hierarchy of systems of inquiry has been reconstructed to become a more useful guide to educational strategies: Each contingent methodology can now be seen to have its own learning, meta-learning, and epistemic learning dimensions. A key to enriched learning for responsible changes in agriculture and rural development lies in the facilitation of the consciousness of, and competency at, such a systemic pluralism of methodologies.     

Complex reorganization and predominant non-homologous repair following chromosomal breakage in karyotypically balanced germline rearrangements and transgenic integration

We defined the genetic landscape of balanced chromosomal rearrangements at nucleotide resolution by sequencing 141 breakpoints from cytogenetically interpreted translocations and inversions. We confirm that the recently described phenomenon of 'chromothripsis' (massive chromosomal shattering and reorganization) is not unique to cancer cells but also occurs in the germline, where it can resolve to a relatively balanced state with frequent inversions. We detected a high incidence of complex rearrangements (19.2%) and substantially less reliance on microhomology (31%) than previously observed in benign copy-number variants (CNVs). We compared these results to experimentally generated DNA breakage-repair by sequencing seven transgenic animals, revealing extensive rearrangement of the transgene and host genome with similar complexity to human germline alterations. Inversion was the most common rearrangement, suggesting that a combined mechanism involving template switching and non-homologous repair mediates the formation of balanced complex rearrangements that are viable, stably replicated and transmitted unaltered to subsequent generations.     

Book Review: Systemic Intervention: Philosophy,Methodology and Practice,Gerald Midgley,Kluwer Academic/Plenum Publishers,New York, 2000, 447 pp., $40, £28 (cloth), ISBN 0-306-46488-8 (cloth)

Systemic Practice and Action Research -     

Tectonic contraction across Los Angeles after removal of groundwater pumping effects

After the 1987 Whittier Narrows and 1994 Northridge earthquakes revealed that blind thrust faults represent a significant threat to metropolitan Los Angeles, a network of 250 continuously recording global positioning system (GPS) stations was deployed to monitor displacements associated with deep slip on both blind and surface faults. Here we augment this GPS data with interferometric synthetic aperture radar imagery to take into account the deformation associated with groundwater pumping and strike-slip faulting. After removing these non-tectonic signals, we are left with 4.4 mm yr-1 of uniaxial contraction across the Los Angeles basin, oriented N 36 degrees E (perpendicular to the major strike-slip faults in the area). This indicates that the contraction is primarily accommodated on thrust faults rather than on the northeast-trending strike-slip faults. We have found that widespread groundwater and oil pumping obscures and in some cases mimics the tectonic signals expected from the blind thrust faults. In the 40-km-long Santa Ana basin, groundwater withdrawal and re-injection produces 12 mm yr-1 of long-term subsidence, accompanied by an unprecedented seasonal oscillation of 55 mm in the vertical direction and 7 mm horizontally.