排序方式: 共有16条查询结果,搜索用时 15 毫秒
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Wright FA Strug LJ Doshi VK Commander CW Blackman SM Sun L Berthiaume Y Cutler D Cojocaru A Collaco JM Corey M Dorfman R Goddard K Green D Kent JW Lange EM Lee S Li W Luo J Mayhew GM Naughton KM Pace RG Paré P Rommens JM Sandford A Stonebraker JR Sun W Taylor C Vanscoy LL Zou F Blangero J Zielenski J O'Neal WK Drumm ML Durie PR Knowles MR Cutting GR 《Nature genetics》2011,43(6):539-546
A combined genome-wide association and linkage study was used to identify loci causing variation in cystic fibrosis lung disease severity. We identified a significant association (P = 3.34 × 10(-8)) near EHF and APIP (chr11p13) in p.Phe508del homozygotes (n = 1,978). The association replicated in p.Phe508del homozygotes (P = 0.006) from a separate family based study (n = 557), with P = 1.49 × 10(-9) for the three-study joint meta-analysis. Linkage analysis of 486 sibling pairs from the family based study identified a significant quantitative trait locus on chromosome 20q13.2 (log(10) odds = 5.03). Our findings provide insight into the causes of variation in lung disease severity in cystic fibrosis and suggest new therapeutic targets for this life-limiting disorder. 相似文献
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Montuori N Bifulco K Carriero MV La Penna C Visconte V Alfano D Pesapane A Rossi FW Salzano S Rossi G Ragno P 《Cellular and molecular life sciences : CMLS》2011,68(14):2453-2467
The receptor (CXCR4) for the stromal-derived factor-1 (SDF1) and the urokinase-receptor (uPAR) are up-regulated in various tumors. We show that CXCR4-transfected cells migrate toward SDF1 on collagen (CG) and do not on vitronectin (VN). Co-expression of cell-surface uPAR, which is a VN receptor, impairs SDF1-induced migration on CG and allows migration on VN. Blocking fMLP receptors (fMLP-R), alpha-v integrins or the uPAR region capable to interact with fMLP-Rs, impairs migration of uPAR/CXCR4-transfected cells on VN and restores their migration on CG. uPAR co-expression also reduces the adherence of CXCR4-expressing cells to various components of the extracellular matrix (ECM) and influences the partitioning of beta1 and alpha-v integrins to membrane lipid-rafts, affecting ECM-dependent signaling. uPAR interference in CXCR4 activity has been confirmed in cells from prostate carcinoma. Our results demonstrate that uPAR expression regulates the adhesive and migratory ability of CXCR4-expressing cells through a mechanism involving fMLP receptors and alpha-v integrins. 相似文献
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M. Aglietta A. Stacchini F. Sanavio W. Piacibello 《Cellular and molecular life sciences : CMLS》1985,41(3):375-376
Summary The effect of 2 H2 receptor antagonists (ranitidine and cimetidine) on the in vitro growth of human granulomonopoietic precursors (CFU-GM) was studied. Ranitidine, although having an anti H2 receptor activity much greater than that of cimetidine, displays the same toxicity for CFU-GM.Acknowledgments. This work was supported by CNR, Rome, PFCCN and AIRC, Milan. 相似文献
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The effect of lithium on normal and chronic granulocytic leukemia (CGL) granulo-monocytic precursors (CFU-GM) has been studied. Lithium slightly increases normal CFU-GM growth whereas it is without effect or moderately inhibitory for CGL CFU-GM. It is suggested that it is unlikely that lithium therapy enhances the proliferation of a silent leukemic clone. 相似文献
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基于遗传BP神经网络的区域物流需求预测 总被引:1,自引:0,他引:1
胡万达 《重庆三峡学院学报》2014,(5)
针对当前区域物流需求预测数据复杂且可变性较大、预测方法环境适应性较差的问题,提出了基于遗传BP神经网络的区域物流需求预测模型。首先,分析区域物流需求预测影响因素,并建立区域物流需求预测指标体系;其次,采用遗传算法优化预测网络中的可变参数,并建立多输入-多输出的BP神经网络多元预测模型;最后,通过实例结果表明该模型具有较高的预测精度和有效度。 相似文献
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Wanda Krawczyńska Aleksandra Przełecka 《Cellular and molecular life sciences : CMLS》1973,29(10):1277-1280
Résumé Des petits fragments de foie de rat ont été maintenus dans un tampon 2 mM tris HCl avec ou sans saccharose (concentration final 0,25M). L'incubation du tissus pendant au moins 1 h dans le tampontris cause une décondensation de la chromatine, qui peut être retardée par le saccharose. Les changements morphologiques des types de chromatine n'ont pas l'effet d'incorporer l'uridine dans le noyaux interphasiques du foie. 相似文献
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热芯盒树脂是在铸造技术中应用比较广泛的一种粘结剂,但是成本较高,本文介绍的一种通过加入低成本的改性剂而研制成的低成本热芯盒树脂,基本上达到国内外同类产品水平,在生产上原料来源广泛,有一定的实际意义。 相似文献
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Sun L Rommens JM Corvol H Li W Li X Chiang TA Lin F Dorfman R Busson PF Parekh RV Zelenika D Blackman SM Corey M Doshi VK Henderson L Naughton KM O'Neal WK Pace RG Stonebraker JR Wood SD Wright FA Zielenski J Clement A Drumm ML Boëlle PY Cutting GR Knowles MR Durie PR Strug LJ 《Nature genetics》2012,44(5):562-569
Variants associated with meconium ileus in cystic fibrosis were identified in 3,763 affected individuals by genome-wide association study (GWAS). Five SNPs at two loci near SLC6A14 at Xq23-24 (minimum P = 1.28 × 10(-12) at rs3788766) and SLC26A9 at 1q32.1 (minimum P = 9.88 × 10(-9) at rs4077468) accounted for ~5% of phenotypic variability and were replicated in an independent sample of affected individuals (n = 2,372; P = 0.001 and 0.0001, respectively). By incorporating the knowledge that disease-causing mutations in CFTR alter electrolyte and fluid flux across surface epithelium into a hypothesis-driven GWAS (GWAS-HD), we identified associations with the same SNPs in SLC6A14 and SLC26A9 and established evidence for the involvement of SNPs in a third solute carrier gene, SLC9A3. In addition, GWAS-HD provided evidence of association between meconium ileus and multiple genes encoding constituents of the apical plasma membrane where CFTR resides (P = 0.0002; testing of 155 apical membrane genes jointly and in replication, P = 0.022). These findings suggest that modulating activities of apical membrane constituents could complement current therapeutic paradigms for cystic fibrosis. 相似文献
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Summary Amino acid acceptor activity of tRNA in the human placenta as measured throughout gestation was found to be the lowest in post-term placenta. Aminoacylation of tRNA proceeded with maximum activity in the stage of formation of the placenta. 相似文献