Nucleotide sequence of the rat skeletal muscle actin gene

The actins constitute a family of highly conserved proteins found in all eukaryotic cells. Their conservation through a very wide range of taxonomic groups and the existence of tissue-specific isoforms make the actin genes very interesting for the study of the evolution of genes and their controlling elements. On the basis of amino acid sequence data, at least six different mammalian actins have been identified (skeletal muscle, cardiac muscle, two smooth muscle actins and the cytoplasmic beta- and gamma-actins). Rat spleen DNA digested by the EcoRI restriction enzyme contains at least 12 different fragments with actin-like sequences but only one which hybridized, in very stringent conditions, with the skeletal muscle cloned cDNA probe. Here we describe the sequence of the actin gene in that fragment. The nucleotide sequence codes for two amino acids, Met-Cys, preceding the known N-terminal Asp of the mature protein. There are five small introns in the coding region and a large intron in the 5'-untranslated region. Comparison of the structure of the rat skeletal muscle actin gene with available data on actin genes from other organisms shows that while the sequenced actin genes from Drosophila and yeast have introns at different locations, introns located at codons specifying amino acids 41, 121, 204 and 267 have been preserved at least from the echinoderm to the vertebrates. A similar analysis has been done by Davidson. An intron at codon 150 is common to a plant actin gene and the skeletal muscle acting gene.     

Why are Three-dimensional Organisms Composed of Two-dimensional Layers?

Multicellular organisms are ensembles of quasi-two-dimensional structures (sheets) of various kinds. Why should the development of all organisms be mediated by a quasi-two-dimensional structure? Why does such development avoid a direct confrontation with the third dimension? In this paper, we accept the challenge of addressing this question from the perspective of computational geometry and suggest that the construction of three-dimensional organisms may be explained by the constraints imposed on a bottom-up construction process.     

Acetylcholine receptors in the gastrulating chick embryo

Summary Muscarinic acetylcholine receptors are present in the gastrulating chick embryo.     

How Deep is the Surface? A Theoretical Framework for Understanding Meaning-Making in Living Systems

Living systems are characterized by unique properties that make them resistant to the ``information-processingperspective' of traditional cognitive science.This paper details those unique properties andoffers a new theoretical framework forunderstanding the behavior of living systems.This framework leans heavily on ideas fromgeneral systems theory (specifically Bateson'sinteractionist perspective), semiotics, andMerleau-Ponty's phenomenology. The benefits ofusing this framework are illustrated withexamples from two different domains: immunologyand verbal interaction.     

Heparanase is preferentially expressed in human psoriatic lesions and induces development of psoriasiform skin inflammation in mice

Heparanase is the sole mammalian endoglycosidase that selectively degrades heparan sulfate, the key polysaccharide associated with the cell surface and extracellular matrix of a wide range of tissues. Extensively studied for its capacity to promote cancer progression, heparanase enzyme was recently implicated as an important determinant in several inflammatory disorders as well. Applying immunohistochemical staining, we detected preferential expression of heparanase by epidermal keratinocytes in human psoriatic lesions. To investigate the role of the enzyme in the pathogenesis of psoriasis, we utilized heparanase transgenic mice in a model of 12-O-tetradecanoyl phorbol 12-myristate 13-acetate-induced cutaneous inflammation. We report that over-expression of the enzyme promotes development of mouse skin lesions that strongly recapitulate the human disease in terms of histomorphological appearance and molecular/cellular characteristics. Importantly, heparanase of epidermal origin appears to facilitate abnormal activation of skin-infiltrating macrophages, thus generating psoriasis-like inflammation conditions, characterized by induction of STAT3, enhanced NF-κB signaling, elevated expression of TNF-α and increased vascularization. Taken together, our results reveal, for the first time, involvement of heparanase in the pathogenesis of psoriasis and highlight a role for the enzyme in facilitating abnormal interactions between immune and epithelial cell subsets of the affected skin. Heparanase inhibitors (currently under clinical testing in malignant diseases) could hence turn highly beneficial in psoriatic patients as well.     

Cyclic nucleotides phosphodiesterase activity changes in early chick development

Differences in the activity of cyclic nucleotides phosphodiesterase develop in different germ layers during the gastrulation of the chick embryo.