CO-binding pigment (P-450) and other electron transport components in hepatoma bearing rats

Zusammenfassung Basierend auf früheren Eiweissuntersuchungen bezüglich einer endoplasmatisch-retikulären Elektronenstromkette ergibt sich: NADPH-Oxydase und Zytochrom-c-Reduktase sind in der normalen Rattenleber wie in der Hepatoma (Typ 7777) tragenden gleich, während die Ferricyanid-Reduktase und P-450 in der Leber der letzteren herabgesetzt waren.     

Effect of sucrose on lipogenesis of rats chronically treated with ethanol

The effect of chronic ethanol administration with and without sucrose on the lipogenic enzymes of liver and adipose tissue of rats was studied. Ethanol markedly influenced the adipose lipogenic enzymes at 28 days. Sucrose caused a 2-10fold increase in lipogenic enzymes of both adipose and liver.     

Spontaneous DNA breakage in single living Escherichia coli cells

Spontaneous DNA breakage is predicted to be a frequent, inevitable consequence of DNA replication and is thought to underlie much of the genomic change that fuels cancer and evolution. Despite its importance, there has been little direct measurement of the amounts, types, sources and fates of spontaneous DNA lesions in living cells. We present a direct, sensitive flow cytometric assay in single living Escherichia coli cells for DNA lesions capable of inducing the SOS DNA damage response, and we report its use in quantification of spontaneous DNA double-strand breaks (DSBs). We report efficient detection of single chromosomal DSBs and rates of spontaneous breakage approximately 20- to 100-fold lower than predicted. In addition, we implicate DNA replication in the origin of spontaneous DSBs with the finding of fewer spontaneous DSBs in a mutant with altered DNA polymerase III. The data imply that spontaneous DSBs induce genomic changes and instability 20-100 times more potently than previously appreciated. Finally, FACS demonstrated two main cell fates after spontaneous DNA damage: viability with or without resumption of proliferation.     

Effect of sodium fluoride on the epinephrine response of liver and hepatoma adenyl cyclase

Zusammenfassung Die in vitro Stimulation der Adenyl-Cyclase durch Epinephrin zur Bildung der zyklischen AMP ist im Hepatomgewebe signifikant höher als in der normalen Rattenleber. Sodiumfluorid hemmt in beiden Fällen die Stimulation.     

Effect of sucrose on lipogenesis of rats chronically treated with ethanol

Summary The effect of chronic ethanol administration with and without sucrose on the lipogenic enzymes of liver and adipose tissue of rats was studied. Ethanol markedly influenced the adipose lipogenic enzymes at 28 days. Sucrose caused a 2-10fold increase in lipogenic enzymes of both adipose and liver.Acknowledgment. This work was supported in part by a grant from the North Carolina Alcohol Research Authority.