Carnosine-like immunoreactivity in the primary olfactory neuron of the rat

Summary Using the peroxidase-antiperoxidase immunohistochemical technique, carnosine-like immunoreactivity was demonstrated to localize specifically within the primary olfactory neuron.This study was supported by grants from the Ministry of Education, Science and Culture, Japan (No. 61218016). Reprint requests to I. N., Department of Anatomy, School of Medicine, Fujita-Gakuen Health University, Toyoake, Aichi 470-11, Japan.     

Reduction of serum X-prolyl dipeptidyl-aminopeptidase activity in tumour-bearing mice and reversal of reduced enzyme activity by lentinan,an anti-tumour polysaccharide

Summary Serum X-prolyl dipeptidyl-aminopeptidase activity which had been shown to be depressed in cancer patients was clearly reduced in mice with Ehrlich carcinoma and Sarcoma 180, and slightly reduced in mice with methylcholanthrene-induced sarcomas. The reduced enzyme activity was completely reversed during tumour regression of sarcoma 180 by administration of lentinan, which causes regression of sarcoma 180.     

Subcellular distribution of tyrosine hydroxylase and monoamine oxidase in the bovine caudate nucleus

Zusammenfassung Die subzelluläre Lokalisation von Tyrosin-Hydroxylase und Monoaminoxydase im Nucleus caudatus des Rindergehirns wurde untersucht. Tyrosin-Hydroxylase fand sich grösstenteils in den Synaptosomen, den Mikrosomen und dem Zytoplasma. Eine bedeutende Aktivität von Monoaminoxydase war in den Mitochondrien vorhanden.     

Dopamine-beta-hydroxylase activity in stroke-prone spontaneously hypertensive rats

Dopamine-beta-hydroxylase (DBH) activity was higher in the serum, the mesenteric artery and the cerebral cortex of 4-week-old stroke-prone spontaneously hypertensive rats (SHRSP), and lower in the nucleus tractus solitarii than it was in spontaneously hypertensive rats (SHR).     

Comparison of X-prolyl dipeptidyl-aminopeptidase activity in human cerebrospinal fluid with that in serum

Summary X-Prolyl dipeptidyl-aminopeptidase activities in cerebrospinal fluid and serum from the same patients without neurological diseases, undergoing surgery under lumbar anesthesia, were assayed fluorometrically with a newly synthesized fluorogenic substrate, 7-glycylproline-4-methylcoumarinamide; the values were 129.1±19.5 nmoles/min/1 and 54.17±3.11 moles/min/1 (mean±SEM, n=23), respectively, and there was no correlation between both activities (r=0.0894).     

Studies on substrate specificity of X-prolyl dipeptidyl-aminopeptidase during new chromogenic substrates, X-Y-p-nitroanilides

Substrate specificity of X-prolyl dipeptidyl-aminopeptidase (dipeptidyl aminopeptidase IV) was examined by using newly synthesized 8 chromogenic substrates, X-Y-p-nitroanilides. Homogeneous enzyme from human submaxillary gland hydrolyzed glycylproline p-nitroanilide almost specifically, except alanylalanine p-nitroanilide which had 11% activity.     

Hydrolysis of amino acidβ-naphthylamides by aminopeptidases in human parotid saliva and human serum

Zusammenfassung Die Aktivität der Aminopeptidasen im menschlichen Speichel von Ohrspeicheldrüsen wurde durch Fluoreszenzanalyse gemessen. Die Substratspezifität der Speichelaminopeptidasen war derjenigen der Serumenzyme ähnlich. Das Glycyl-Prolin -naphthylamidspaltende Enzym war jedoch im Speichel von Ohrspeicheldrüsen in relativ grösserer Menge vorhanden.     

Serum dopamine-β-hydroxylase in spontaneously hypertensive rats

Zusammenfassung Es wird gezeigt, dass die Dopamin--Hydroxylase-Aktivität im Serum bei spontan hypertonischen Ratten im Vergleich zu normotonen Kontrollen nicht verändert ist.