Mesenchymal–epithelial interactions during digestive tract development and epithelial stem cell regeneration

The gastrointestinal tract develops from a simple and uniform tube into a complex organ with specific differentiation patterns along the anterior–posterior and dorso-ventral axes of asymmetry. It is derived from all three germ layers and their cross-talk is important for the regulated development of fetal and adult gastrointestinal structures and organs. Signals from the adjacent mesoderm are essential for the morphogenesis of the overlying epithelium. These mesenchymal–epithelial interactions govern the development and regionalization of the different gastrointestinal epithelia and involve most of the key morphogens and signaling pathways, such as the Hedgehog, BMPs, Notch, WNT, HOX, SOX and FOXF cascades. Moreover, the mechanisms underlying mesenchyme differentiation into smooth muscle cells influence the regionalization of the gastrointestinal epithelium through interactions with the enteric nervous system. In the neonatal and adult gastrointestinal tract, mesenchymal–epithelial interactions are essential for the maintenance of the epithelial regionalization and digestive epithelial homeostasis. Disruption of these interactions is also associated with bowel dysfunction potentially leading to epithelial tumor development. In this review, we will discuss various aspects of the mesenchymal–epithelial interactions observed during digestive epithelium development and differentiation and also during epithelial stem cell regeneration.     

Effets immédiats de quelques antibiotiques sur l'électro-osmose chezNitella flexilis L.

Summary The short term effects of chloramphenicol, polymyxin sulfate and oligomycin A + B (the latter in 0,5M alcoholic solution) on the basic flow and the electrosmosis inNitella were studied. Both chloramphenicol and polymyxin increase the basic flow but only polymyxin increases the electro-osmotic efficiency.     

Timescales of shock processes in chondritic and martian meteorites

The accretion of the terrestrial planets from asteroid collisions and the delivery to the Earth of martian and lunar meteorites has been modelled extensively. Meteorites that have experienced shock waves from such collisions can potentially be used to reveal the accretion process at different stages of evolution within the Solar System. Here we have determined the peak pressure experienced and the duration of impact in a chondrite and a martian meteorite, and have combined the data with impact scaling laws to infer the sizes of the impactors and the associated craters on the meteorite parent bodies. The duration of shock events is inferred from trace element distributions between coexisting high-pressure minerals in the shear melt veins of the meteorites. The shock duration and the associated sizes of the impactor are found to be much greater in the chondrite (approximately 1 s and 5 km, respectively) than in the martian meteorite (approximately 10 ms and 100 m). The latter result compares well with numerical modelling studies of cratering on Mars, and we suggest that martian meteorites with similar, recent ejection ages (10(5) to 10(7) years ago) may have originated from the same few square kilometres on Mars.     

Microwave devices: carbon nanotubes as cold cathodes

To communicate, spacecraft and satellites rely on microwave devices, which at present are based on relatively inefficient thermionic electron sources that require heating and cannot be switched on instantaneously. Here we describe a microwave diode that uses a cold-cathode electron source consisting of carbon nanotubes and that operates at high frequency and at high current densities. Because it weighs little, responds instantaneously and has no need of heating, this miniaturized electron source should prove valuable for microwave devices used in telecommunications.     

Variations de la différence de potentiel électrochimique des chlorures chezNitella en présence de benzène sulphonate

Summary The chloride electrochemical potential difference between the inside of cells ofNitella translucens and some external solutions, where Cl has been progressively replaced by benzenesulphonate, has been measured by means of Ag-AgCl electrodes. It appears that benzenesulphonate moves into the cell by an electrogenic pump and that the uptake of Cl byNitella is not in contradiction with a carrier hypothesis.

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This work was supported by grants from the F.N.R.S. and was carried out in the Biophysics Section of the Edinburgh University. We are indebted to Dr. E. J.Williams and Dr.Hogg for having sponsored this research and kindly provided the material.     

Modification de la vitesse du courant cytoplasmique,induite à distance,par l'irradiation,chezNitella flexilis

Summary Irradiation of an internodal cell ofNitella flexilis induces in the adjacent internodal cell a transitory acceleration of cytoplasmic streaming; during 8 h at least the speed of this streaming is higher than in control internodal cells. It is probable that this phenomenon is due to transfer of certain substances from the irradiated cell.     

Rad51 paralogues Rad55-Rad57 balance the antirecombinase Srs2 in Rad51 filament formation

Homologous recombination is a high-fidelity DNA repair pathway. Besides a critical role in accurate chromosome segregation during meiosis, recombination functions in DNA repair and in the recovery of stalled or broken replication forks to ensure genomic stability. In contrast, inappropriate recombination contributes to genomic instability, leading to loss of heterozygosity, chromosome rearrangements and cell death. The RecA/UvsX/RadA/Rad51 family of proteins catalyses the signature reactions of recombination, homology search and DNA strand invasion. Eukaryotes also possess Rad51 paralogues, whose exact role in recombination remains to be defined. Here we show that the Saccharomyces cerevisiae Rad51 paralogues, the Rad55-Rad57 heterodimer, counteract the antirecombination activity of the Srs2 helicase. The Rad55-Rad57 heterodimer associates with the Rad51-single-stranded DNA filament, rendering it more stable than a nucleoprotein filament containing Rad51 alone. The Rad51-Rad55-Rad57 co-filament resists disruption by the Srs2 antirecombinase by blocking Srs2 translocation, involving a direct protein interaction between Rad55-Rad57 and Srs2. Our results demonstrate an unexpected role of the Rad51 paralogues in stabilizing the Rad51 filament against a biologically important antagonist, the Srs2 antirecombination helicase. The biological significance of this mechanism is indicated by a complete suppression of the ionizing radiation sensitivity of rad55 or rad57 mutants by concomitant deletion of SRS2, as expected for biological antagonists. We propose that the Rad51 presynaptic filament is a meta-stable reversible intermediate, whose assembly and disassembly is governed by the balance between Rad55-Rad57 and Srs2, providing a key regulatory mechanism controlling the initiation of homologous recombination. These data provide a paradigm for the potential function of the human RAD51 paralogues, which are known to be involved in cancer predisposition and human disease.