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Bennett IM Farfano HM Bogani F Primak A Liddell PA Otero L Sereno L Silber JJ Moore AL Moore TA Gust D 《Nature》2002,420(6914):398-401
Transport of calcium ions across membranes and against a thermodynamic gradient is essential to many biological processes, including muscle contraction, the citric acid cycle, glycogen metabolism, release of neurotransmitters, vision, biological signal transduction and immune response. Synthetic systems that transport metal ions across lipid or liquid membranes are well known, and in some cases light has been used to facilitate transport. Typically, a carrier molecule located in a symmetric membrane binds the ion from aqueous solution on one side and releases it on the other. The thermodynamic driving force is provided by an ion concentration difference between the two aqueous solutions, coupling to such a gradient in an auxiliary species, or photomodulation of the carrier by an asymmetric photon flux. Here we report a different approach, in which active transport is driven not by concentration gradients, but by light-induced electron transfer in a photoactive molecule that is asymmetrically disposed across a lipid bilayer. The system comprises a synthetic, light-driven transmembrane Ca2+ pump based on a redox-sensitive, lipophilic Ca2+-binding shuttle molecule whose function is powered by an intramembrane artificial photosynthetic reaction centre. The resulting structure transports calcium ions across the bilayer of a liposome to develop both a calcium ion concentration gradient and a membrane potential, expanding Mitchell's concept of a redox loop mechanism for protons to include divalent cations. Although the quantum yield is relatively low (approximately 1 per cent), the Ca2+ electrochemical potential developed is significant. 相似文献
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E. de Conti C. L. Messias Hebe Myrina L. de Souza J. L. Azevedo 《Cellular and molecular life sciences : CMLS》1980,36(3):293-294
Summary 11 wild-type strains ofMetarrhizium anisopliae were electrophoretically tested for phosphatases and esterases. Phosphatase was homogeneous for all strains. For esterase it was possible to distinguish 5 different patterns with which it is possible to characterize the strains analyzed.Acknowledgment. The authors are thankful to the Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) and to the Empresa Brasileira de Pesquisa Agropecuária (EM-BRAPA) for financial assistance. 相似文献
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