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Gait MJ 《Cellular and molecular life sciences : CMLS》2003,60(5):844-853
Improving the delivery of synthetic oligonucleotides and their analogues into cells is an important goal in the full development of antisense technology for control of gene expression in cell culture and in vivo. This review describes the harnessing of certain peptides, either as noncovalent complexes or as covalent conjugates, to enhance the delivery of antisense oligonucleotides into cells and/or to affect their cell localization. Phosphodiester and phosphorothioate oligonucleotides are included as well as peptide nucleic acids (PNAs), analogues of oligonucleotides where the negatively charged phosphate backbone is replaced by a neutral amide linkage. This review contains a critical evaluation of claims for certain peptide-oligonucleotide conjugates to translocate into cultured cells by a non-energy-dependent nonendosomal route. In addition, the available evidence for the utility of stable versus nonstable linkages between peptide and oligonucleotide or PNA is discussed. 相似文献
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Cell penetrating peptides: overview and applications to the delivery of oligonucleotides 总被引:1,自引:1,他引:0
F. Said Hassane A. F. Saleh R. Abes M. J. Gait Bernard Lebleu 《Cellular and molecular life sciences : CMLS》2010,67(5):715-726
Crossing biological barriers represents a major limitation for clinical applications of biomolecules such as nucleic acids,
peptides or proteins. Cell penetrating peptides (CPP), also named protein transduction domains, comprise short and usually
basic amino acids-rich peptides originating from proteins able to cross biological barriers, such as the viral Tat protein,
or are rationally designed. They have emerged as a new class of non-viral vectors allowing the delivery of various biomolecules
across biological barriers from low molecular weight drugs to nanosized particles. Encouraging data with CPP-conjugated oligonucleotides
have been obtained both in vitro and in vivo in animal models of diseases such as Duchenne muscular dystrophy. Whether CPP-cargo
conjugates enter cells by direct translocation across the plasma membrane or by endocytosis remains controversial. In many
instances, however, endosomal escape appears as a major limitation of this new delivery strategy. 相似文献
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