Chromatographic separation of an inhibitor reversibly associated with fibrinolysin in the serum of guinea-pig

Riassunto Il solfato di cellulosa attiva l'enzima fibrinolitico del siero di cavia in soluzione neutra, a differenza di altri poliacidi che sono attivatori a basso pH. È proposto un meccanismo per l'attivazione basato sull'interazione fra il poliacido e l'inibitore. L'inibitore è stato separato dalle globuline di cavia per cromatografia a scambio di ioni.     

The effect of sea water on cytochrome oxidase and oxidative phosphorylation

Riassunto Piccole quantità di acqua di mare causano una forte inibizione della fosforilazione ossidativa, mentre l'effetto sulla attività citocromossidasica è piuttosto modesto. L'ione responsabile di questo effetto è il Calcio.

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This work has been supported in part by a grant of the National Institute of Health, U.S. Public Health Service (RG-6211) to the Laboratory of Comparative Anatomy.     

Enzymic oxidation of aminoketones in mammalian blood plasma

Zusammenfassung Rinderserum oxydiert Aminoaceton rasch und-Aminolävulinsäure langsam, Reaktionen, die wahrscheinlich von der Sperminoxydase katalysiert werden. Aminoketone werden jedoch nicht im Plasma aller Tierarten oxydiert, welche Spermin oxydieren. Plasma von Tierarten, in denen Benzylaminoxydase vorkommt, oxydieren in der Regel ebenfalls Aminoaceton. Eine Ausnahme: Die Benzylaminoxydase des Schweines greift die beiden Aminoketone nicht merklich an.     

Deep sub-seafloor prokaryotes stimulated at interfaces over geological time

The sub-seafloor biosphere is the largest prokaryotic habitat on Earth but also a habitat with the lowest metabolic rates. Modelled activity rates are very low, indicating that most prokaryotes may be inactive or have extraordinarily slow metabolism. Here we present results from two Pacific Ocean sites, margin and open ocean, both of which have deep, subsurface stimulation of prokaryotic processes associated with geochemical and/or sedimentary interfaces. At 90 m depth in the margin site, stimulation was such that prokaryote numbers were higher (about 13-fold) and activity rates higher than or similar to near-surface values. Analysis of high-molecular-mass DNA confirmed the presence of viable prokaryotes and showed changes in biodiversity with depth that were coupled to geochemistry, including a marked community change at the 90-m interface. At the open ocean site, increases in numbers of prokaryotes at depth were more restricted but also corresponded to increased activity; however, this time they were associated with repeating layers of diatom-rich sediments (about 9 Myr old). These results show that deep sedimentary prokaryotes can have high activity, have changing diversity associated with interfaces and are active over geological timescales.     

Distribution of radioactivity after administration of3H-5-hydroxytryptamine by three different routes to the musselMytilus edulis

Summary Intracardiac injection gives proportionately higher and earlier peak concentration in tissues that specifically accumulates it, i.m. injection gives slower but longer lasting accumulations, and topical application to a ganglia essentially localizes the drug in that structure.We wish to thank Drs Hiripi and Nemcsok for the demonstration of the intracardiac technique. We also wish to thank Drs Haley and Ledeen for the use of the scintillation counter.     

2-Amino-6-[(1'R,2'S)-1',2'-dihydroxypropyl]-3-methyl-pterin-4-one,a biologically active metabolite from the anthozoanAstroides calycularis Pallas

Summary 2-Amino-6-[(1'R,2'S)-1',2'-dihydroxypropyl]-3-methyl-pterin-4-one (1) has been isolated from the marine anthozoanAstroides calycularis; its structure was illustrated by spectral analyses including 2D-NMR and by partial synthesis.1 appears to possess cell-growth inhibiting activity.29 December 1986Work supported by C.N.R. (Progetto Finalizzato Chimica Fine e Secondaria) and by Ministero Pubblica Istruzione Italy. — Acknowledgments. Mass spectral data were provided by Servizio di Spettrometria di massa del C.N.R. e dell'Università di Napoli. The assistance of the staff is gratefully appreciated. Finally we would like to thank Prof. C. Pietropaolo for supplying chick embryo fibroblasts.