Genome sequence of enterohaemorrhagic Escherichia coli O157:H7

The bacterium Escherichia coli O157:H7 is a worldwide threat to public health and has been implicated in many outbreaks of haemorrhagic colitis, some of which included fatalities caused by haemolytic uraemic syndrome. Close to 75,000 cases of O157:H7 infection are now estimated to occur annually in the United States. The severity of disease, the lack of effective treatment and the potential for large-scale outbreaks from contaminated food supplies have propelled intensive research on the pathogenesis and detection of E. coli O157:H7 (ref. 4). Here we have sequenced the genome of E. coli O157:H7 to identify candidate genes responsible for pathogenesis, to develop better methods of strain detection and to advance our understanding of the evolution of E. coli, through comparison with the genome of the non-pathogenic laboratory strain E. coli K-12 (ref. 5). We find that lateral gene transfer is far more extensive than previously anticipated. In fact, 1,387 new genes encoded in strain-specific clusters of diverse sizes were found in O157:H7. These include candidate virulence factors, alternative metabolic capacities, several prophages and other new functions--all of which could be targets for surveillance.     

半胱氨酸亚金铵的合成、理化性质及电镀金应用

以半胱氨酸为配体合成一种新型亚金配合物NH4Au(Cys)2,对该配合物进行元素分析、红外光谱、紫外光谱、热失重分析和导电性测量等理化性质研究;以该亚金配合物为金源开展相关的电镀金工艺探索,并通过四因素三水平的正交试验获得其最佳条件参数;采用扫描电子显微镜(SEM)和X线衍射(XRD)对镀金层的表面质量进行探讨。研究结果表明:该目标产物的分子式为NH4Au(Cys)2·2H2O,该配合物中以半胱氨酸的巯基和金配位为成健特征,在170℃以下热稳定性较好,该亚金配合物是一个典型的离子化合物。在电流密度为200~300 A/m2,p H为10.5~12.0,温度为35~45℃,金质量浓度为15~25 g/L的电镀工艺条件下,得到粒度为0.5~1.0μm的单质金,且主要沿着(111)面进行生长。     

随机纳米碳管网络及其渗流性质

数值模拟了实验上构造纳米碳管网络的溶液沉积方法.与一般的随机网络模型不同,将碳管的长度计算在内,而且考虑了不同的空间相交位形.数值模拟发现网络的度分布为高斯分布,平均集聚系数约为0.11.当网络中碳管平均面密度取值在σ0=179 200根/cm2附近时,网络系综达到渗流.在临界点附近,网络的连通概率p、两极之间电导G、...     

十六位微机教学实验系统分析与改进

本文在分析CCT－88／51／98教学实验系统监控程序的基础上，对该教学实验系统监控程序中存在的问题作出了一些改进。     

用模糊综合评判法评价变电所所址

变电所选址是电力系统规划的一个重要环节。但与变电所所址有关的因素错综复杂,很难仅凭经验就能确定最佳所址。本文采用模糊综合评判方法对备选所址进行评判,确定其优先次序。在模糊综合评判中,各因素的权重分配对结果起决定性作用,用层次分析法确定与所址密切相关的各个因素相对总体目标而言的权重,使得权重的确定更科学。算例表明该方法是可行的。     

Genomic sequencing of colorectal adenocarcinomas identifies a recurrent VTI1A-TCF7L2 fusion

Prior studies have identified recurrent oncogenic mutations in colorectal adenocarcinoma and have surveyed exons of protein-coding genes for mutations in 11 affected individuals. Here we report whole-genome sequencing from nine individuals with colorectal cancer, including primary colorectal tumors and matched adjacent non-tumor tissues, at an average of 30.7× and 31.9× coverage, respectively. We identify an average of 75 somatic rearrangements per tumor, including complex networks of translocations between pairs of chromosomes. Eleven rearrangements encode predicted in-frame fusion proteins, including a fusion of VTI1A and TCF7L2 found in 3 out of 97 colorectal cancers. Although TCF7L2 encodes TCF4, which cooperates with β-catenin in colorectal carcinogenesis, the fusion lacks the TCF4 β-catenin-binding domain. We found a colorectal carcinoma cell line harboring the fusion gene to be dependent on VTI1A-TCF7L2 for anchorage-independent growth using RNA interference-mediated knockdown. This study shows previously unidentified levels of genomic rearrangements in colorectal carcinoma that can lead to essential gene fusions and other oncogenic events.     

用脸谱图对太子河本溪市区段河流沉积物中重金属污染进行评价的研究

本文将沉积学原理及国际上新发展的两种重金属污染评价方法与多变量的图表示法——脸谱图相结合,对太子河本溪市区段河道沉积物中重金属的污染状况及潜在生态危害进行了综合性的评价研究。从脸谱图上可以直观地看出各采样点重金属的污染情况和潜在生态危害程度。从研究结果可以看出,太子河本溪市区段河道沉积物中重金属的污染是很严重的。     

寻找四元域上n维线性空间中n/2维自对偶子空间的方法

本文讨论F4上n维线性空间的k维子空间W，这些子空间都有特定的自同构群(实际上是典型群GLn(F4)的一个子群)，根据群中元素形式的不同可将子空间W分为两类，并对寻找n维空间中形如这两类的n／2维自对偶子空间提供了一种采用降低维数寻找的方法。     

Autotaxin

Autotaxin is a protein of approximately 900 amino acids discovered in the early 1990s. Over the past 15 years, a strong association between cancer cells and autotaxin production has been observed. Recent publications indicate that autotaxin and the capacity of cancer to metastasise are intimately linked. The discovery of new molecular targets in pharmacology is a mixture of pure luck, hard work and industrial strategy. Despite a crucial and desperate need for new therapeutic tools, many targets are approached in oncology, but only a few are validated and end up at the patient bed. Outside the busy domain of kinases, few targets have been discovered that can be useful in treating cancer, particularly metastatic processes. The fortuitous relationship between autotaxin and lysophosphatidic acid renders the results of observations made in the diabetes/obesity context considerably important. The literature provides observations that may aid in redesigning experiments to validate autotaxin as a potential oncology target.     

多梯度复杂图像的分割

图像分割是一种重要的图像分析技术,它不仅得到人们广泛的重视和研究,也在实际中得到大量的应用。本文针对一些经典分割算法对多梯度复杂图像分割边缘定位不准确,易受噪声干扰的特点,提出了一种利用图像边缘区域对多梯度复杂图像进行自适应阈值分割的算法。通过对各种算法的比较,本算法抗干扰能力较强,稳定性好,而且完全自动,不需预先设定任何参数。对多种图像的实验表明本文方法十分有效。