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One potential obstacle to effective information systems development involves the conflict between users and developers. It has been argued that information systems development personnel have different perceptions of what constitutes systems effectiveness than do users. System objectives are accomplished from the developer's viewpoint when a system has technical validity. System objectives are accomplished from the user's viewpoint when the system has organizational validity. Differences in the assessment of information systems project success are accentuated when users perceive the project as a failure. Attribution theory, a social psychology theory, is employed here to explain the source and outcome of such conflict. Also discussed are alternative ways of resolving those differences. 相似文献
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B M Hogema M Gupta H Senephansiri T G Burlingame M Taylor C Jakobs R B Schutgens W Froestl O C Snead R Diaz-Arrastia T Bottiglieri M Grompe K M Gibson 《Nature genetics》2001,29(2):212-216
Succinate semialdehyde dehydrogenase (ALDH5A1, encoding SSADH deficiency is a defect of 4-aminobutyric acid (GABA) degradation that manifests in humans as 4-hydroxybutyric (gamma-hydroxybutyric, GHB) aciduria. It is characterized by a non-specific neurological disorder including psychomotor retardation, language delay, seizures, hypotonia and ataxia. The current therapy, vigabatrin (VGB), is not uniformly successful. Here we report the development of Aldh5a1-deficient mice. At postnatal day 16-22 Aldh5a1-/- mice display ataxia and develop generalized seizures leading to rapid death. We observed increased amounts of GHB and total GABA in urine, brain and liver homogenates and detected significant gliosis in the hippocampus of Aldh5a1-/- mice. We found therapeutic intervention with phenobarbital or phenytoin ineffective, whereas intervention with vigabatrin or the GABAB receptor antagonist CGP 35348 (ref. 2) prevented tonic-clonic convulsions and significantly enhanced survival of the mutant mice. Because neurologic deterioration coincided with weaning, we hypothesized the presence of a protective compound in breast milk. Indeed, treatment of mutant mice with the amino acid taurine rescued Aldh5a1-/- mice. These findings provide insight into pathomechanisms and may have therapeutic relevance for the human SSADH deficiency disease and GHB overdose and toxicity. 相似文献
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Wen X Lei YP Zhou YL Okamoto CT Snead ML Paine ML 《Cellular and molecular life sciences : CMLS》2005,62(9):1038-1046
Tuftelin-interacting protein (TFIP11) was first identified in a yeast two-hybrid screening as a protein interacting with tuftelin. The ubiquitous expression of TFIP11 suggested that it might have other functions in non-dental tissues. TFIP11 contains a G-patch, a protein domain believed to be involved in RNA binding. Using a green fluorescence protein tag, TFIP11 was found to locate in a novel subnuclear structure that we refer to as the TFIP body. An in vivo splicing assay demonstrated that TFIP11 is a novel splicing factor. TFIP11 diffuses from the TFIP body following RNase A treatment, suggesting that the retention of TFIP11 is RNA dependent. RNA polymerase II inhibitor (-amanitin and actinomycin D) treatment causes enlargement in size and decrease in number of TFIP bodies, suggesting that TFIP bodies perform a storage function rather than an active splicing function. The TFIP body may therefore represent a new subnuclear storage compartment for splicing components.Received 8 December 2004; received after revision 27 January 2005; accepted 8 March 2004The nucleotide sequence for the cDNA to mouse TFIP11 (previously known as TIP39 and TIP39kDa) has been submitted to Gen- BankTM/ EBI Data Bank with accession numbers AF290474 and NM_018783. The accession number for the human TFIP11 homologueis NM_012143. 相似文献
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Shapiro JL Wen X Okamoto CT Wang HJ Lyngstadaas SP Goldberg M Snead ML Paine ML 《Cellular and molecular life sciences : CMLS》2007,64(2):244-256
Proteins of the developing enamel matrix include amelogenin, ameloblastin and enamelin. Of these three proteins amelogenin
predominates. Protein-protein interactions are likely to occur at the ameloblast Tomes’ processes between membrane-bound proteins
and secreted enamel matrix proteins. Such protein-protein interactions could be associated with cell signaling or endocytosis.
CD63 and Lamp1 are ubiquitously expressed, are lysosomal integral membrane proteins, and localize to the plasma membrane.
CD63 and Lamp1 interact with amelogenin in vitro. In this study our objective was to study the molecular events of intercellular trafficking of an exogenous source of amelogenin,
and related this movement to the spatiotemporal expression of CD63 and Lamp1 using various cell lineages. Exogenously added
amelogenin moves rapidly into the cell into established Lamp1-positive vesicles that subsequently localize to the perinuclear
region. These data indicate a possible mechanism by which amelogenin, or degraded amelogenin peptides, are removed from the
extracellular matrix during enamel formation and maturation.
Received 27 September 2006; received after revision 24 November 2006; accepted 5 December 2006 相似文献
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