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乙醇脱氢酶在聚苯胺-聚丙烯酸修饰电极上的固定及表征   总被引:1,自引:1,他引:1  
采用循环伏安法在铂电极上于0.5mol/L苯胺-2.5mol/LHCI-15%(质量分数)聚丙烯酸的水溶液中制备聚丙烯酸掺杂的聚苯胺膜;并在0.25mol/L磷酸氢二钠-柠檬酸缓冲溶液(pH=7)中测试掺杂后的聚苯胺膜的电化学性能,聚苯胺-聚丙烯酸(PAn-PAA)复合膜修饰电极在电位为-0.2~0.4V时出现1对稳定的氧化还原峰,与PAn修饰电极相比,它在此中性缓冲溶液中具有更好的导电性和电化学活性,可用作固定酶的载体和酶催化反应的电子传递媒介。实验中采用两步法将乙醇脱氢酶(ADH)固定至PAn-PAA复合膜中,用循环伏安(CV)、交流阻抗(EIS)和扫描电镜(SEM)等方法对固定乙醇脱氢酶前后膜的性能进行表征。结果表明ADH已固定至复合膜中。  相似文献   
2.
采用油包水(W/O)微乳液法,以KBH4为还原剂,在水相-Triton X 100-异丙醇-环己烷组成的W/O微乳液中还原二价铁、钴、镍盐,合成Fe-Co-Ni合金纳米微粒。考察反应气氛、温度、时间和洗涤溶液对反应的影响,并用X射线衍射仪、扫描电子显微镜对合成的纳米微粒的形貌、结构进行检测。研究结果表明:在25-30℃的N2中反应1 h,得到的产物待丙酮破乳后用无水乙醇洗涤结果最佳。合成的Fe-Co-Ni合金是粒径为1-3 nm的球状微粒。  相似文献   
3.
Breast metastases from extramammary neoplasms are very rare. We presented a 66 year-old female with metastasis of small cell lung carcinoma to the breast. She presented with consolidation over the left upper lobe of her lung undetermined after endobronchial or video-assisted thoracoscopic surgery (VATS) biopsy, and this was treated effectively after antibiotic therapy at initial stage. The left breast lumps were noted 4 months later, and she underwent a modified radical mastectomy under the impression of primary breast carcinoma. However, the subsequent chest imaging revealed re-growing mass over the left mediastinum and hilum, and cells with the same morphological and staining features were found from specimens oftransbronchial brushing and biopsy. An accurate diagnosis to distinguish a primary breast carcinoma from metastatic one is very important because the therapeutic planning and the outcome between them are different.  相似文献   
4.
In this paper we formulate a bi-criteria search strategy of a heuristic learning algorithm forsolving multiple resource-constrained project scheduling problems. The heuristic solves problems intwo phases. In the pre-processing phase, the algorithm estimates distance between a state and the goalstate and measures complexity of problem instances. In the search phase, the algorithm uses estimatesof the pre-processing phase to further estimate distances to the goal state. The search continues in astepwise generation of a series of intermediate states through search path evaluation process withbacktracking. Developments of intermediate states are exclusively based on a bi-criteria new stateselection technique where we consider resource utilization and duration estimate to the goal state. Wealso propose a variable weighting technique based on initial problem complexity measures.Introducing this technique allows the algorithm to efficiently solve complex project schedulingproblems. A numerical example illustra  相似文献   
5.
提出了不同于传统电动力学的直流电能流输运过程解释,指出坡印廷能流的定义不能简单推广到直流电.通过对含欧姆定律的麦克斯韦方程组进行分析,证明了直流电的能流输运过程受扩散方程支配,与波动方程无关;发现经典坡印廷能流的推导过程存在隐含的物理学限制,导致其不适用于金属导体内电磁纵场的做功过程.研究表明,金属导体直流电能的输运过程及其支配方程均与洛伦茨力有关,与坡印廷矢量无关.研究结论可以推广至低频似稳态,从而为电工学和近场电磁学的研究带来广阔的前景.  相似文献   
6.
A spray drier was designed employing the two-fluid nozzle as, the spraying device complete with a collecter for the powder conveyed by air. No difficulty was encountered in dehydrating the soy-bean milk just as in the case of cow milk. Improving the interaction between .the hot air and the sprayed liquor increased the rate of heat transmission and the percentage of air utilized with higher thermal efficiency as a. result. The thermal efficiency was governed also by the mean temperature difference bet...  相似文献   
7.
研究不同浓度的Cr6+溶液胁迫对番茄种子发芽及幼根生长的影响,以"903号"番茄种子为试材,采用0.01、0.05、0.1、0.5 mmol/L Cr6+溶液处理进行种子的发芽试验,研究种子萌发和生长的影响.试验结果表明:0.01、0.05、0.1、0.5 mmol/L Cr6+溶液处理的番茄种子的发芽率、发芽势、发芽指数、活力指数、幼根长和芽长均低于CK,且随Cr6+溶液浓度的升高而呈下降趋势.Cr6+对番茄种子萌发及幼苗生长有抑制作用.  相似文献   
8.
应用型人才培养的实验教学改革实践   总被引:14,自引:0,他引:14  
针对地方高校实验教学中存在的问题,以调动学生积极性,培养学生实践能力和创新能力为出发点,从提高实验课地位、构建实验教学体系、改进教学方法、改革实验考核方式等方面进行了实践探索,取得了较好的效果。  相似文献   
9.
为了优化金针菇合成多糖的液体发酵培养基配置,本文以金针菇杂交菌种F19为研究对象,以金针菇多糖收率为评价指标,探讨了不同碳源、氮源、无机盐、维生素及其不同配比对金针菇多糖合成的影响,采取正交试验方法得出最佳液体发酵培养基为:马铃薯200 g(去皮煮汁)、葡萄糖10 g、可溶性淀粉30 g、玉米粉30 g、KCl 1.0 g,VB2 100 mg、水1 000 mL.  相似文献   
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