SLC2A9 is a newly identified urate transporter influencing serum urate concentration, urate excretion and gout

Uric acid is the end product of purine metabolism in humans and great apes, which have lost hepatic uricase activity, leading to uniquely high serum uric acid concentrations (200-500 microM) compared with other mammals (3-120 microM). About 70% of daily urate disposal occurs via the kidneys, and in 5-25% of the human population, impaired renal excretion leads to hyperuricemia. About 10% of people with hyperuricemia develop gout, an inflammatory arthritis that results from deposition of monosodium urate crystals in the joint. We have identified genetic variants within a transporter gene, SLC2A9, that explain 1.7-5.3% of the variance in serum uric acid concentrations, following a genome-wide association scan in a Croatian population sample. SLC2A9 variants were also associated with low fractional excretion of uric acid and/or gout in UK, Croatian and German population samples. SLC2A9 is a known fructose transporter, and we now show that it has strong uric acid transport activity in Xenopus laevis oocytes.     

Effect of Supervision on Stress and Burnout in School Counsellors: A Case of Action Research

School counsellors are permanently in action, the success of which depends on their systemic approach to their action. Due to the difficulty, complexity and scope of their work, school counsellors are often under a lot of stress, which may eventually develop into occupational burnout. Thus, it is very important that school counsellors can use appropriate i.e. systemic, i.e. requisitely holistic ways of coping with stress and occupational burnout. Recently, after many years, supervision has been gaining importance as an action in the form of professional help to school counsellors to enable them to cope effectively with stress and to prevent occupational burnout. The primary purpose of the reported-about study is to determine how supervision, in accordance with the relational family model, affects the perception of stress and occupational burnout. The sample included 30 school counsellors in Slovenia. They were divided into an experimental group (with the presence of supervision) and a control group (without supervision). Results show a positive effect of supervision, in accordance with the relational family model, on the school counsellors’ perception of stress, since stress decreased after supervisory meetings, while the school counsellors’ perception of their professional burnout after supervisory meetings did not change significantly.     

Critical Self-Evaluation: An Attribute of Systemic Behavior: Authors of Natural Science Learning Materials as Evaluators

Social responsibility (SR) requires critical self-evaluating personalities. Here is a case of self-measurement. Some basic content-, methodology- and implementation-related characteristics of evaluation in education are presented. Particularly emphasized are attributes of good evaluation and good evaluators. The study empirically surveys the cognitive and emotional–motivational preparedness of e.g. authors of natural sciences learning materials for evaluation of their authored materials. Data analysis shows that authors hesitate. They worry about their objectivity, lack of time, and insufficient methodological training. Authors more generally confirmed their cooperation in the planning of evaluation in terms of content and methodology and evaluation report generation. Method may be usable in other cases, too, and may help its users improve their SR in self-measurement.     

Effect of storage conditions on long-term stability of Ag nanoparticles formed via green synthesis

Spherical Ag nanoparticles (AgNPs) with a diameter of 20 nm or smaller were biologically synthesized using algae Parachlorella kessleri. The effect of storage conditions on the long-term stability of AgNPs was investigated. UV/Vis spectrophotometry, transmission electron microscopy, and dynamic light scattering measurements revealed that the long-term stability of AgNPs was influenced by light and temperature conditions. The most significant loss of stability was observed for the AgNPs stored in daylight at room temperature. The AgNPs stored under these conditions began to lose their stability after approximately 30 d; after 100 d, a substantial amount of agglomerated particles settled to the bottom of the Erlenmeyer flask. The AgNPs stored in the dark at room temperature exhibited better long-term stability. Weak particle agglomeration began at approximately the 100th day. The AgNPs stored in the dark at about 5℃ exhibited the best long-term stability; the AgNPs stored under such conditions remained spherical, with a narrow size distribution, and stable (no agglomeration) even after 6 months. Zeta-potential measurements confirmed better dispersity and stability of AgNPs stored under these conditions.