Ultrastructural investigation of fetal rat brain hemisphere tissue in nonadherent stationary organ culture

Summary Fetal rat brain fragments grown in nonadherent stationary organ culture for 50 days have been investigated ultrastructurally. Synaptogenesis and myelin formation occurred at the same time as the corresponding time-dependent events in the developing brain in vivo. Intermediate junctions were observed between cellular processes lining a central cavity in the fragments and later associated with astrocytes at the surface. Gap junctions and tight junctions were also present. In some fragments cilia were observed in the central cavity. Subependymal basement membrane labyrinths were observed in all fragments after 10 days in culture. The ultrastructural characteristics and the tissue-like structure in general were preserved for at least 50 days in this tissue culture system. The brain fragments may therefore be a valuable supplement to existing culture methods for nervous tissue.Research fellow from the Norwegian Research Council for Sciences and Humanities. This work was supported by the Norwegian Cancer Society.     

家猫精子的超微结构

本文通过扫描电镜,透射电镜对家猫精子的形态结构进行了观察.家猫精子的核占头的绝大部分.顶体是核外的一个薄层.颈部的9个节柱每个有15节.4、5、6、7节柱的前部形成一个植入板,1、2、9形成另一个植入板.近侧中心粒长轴与尾的长轴成46°—70°角.线粒体鞘有28—31旋.致密纤维1、5、6、9常比其他纤维明显增大.主段尾部微管A的二臂、连丝、辐条和二个联体微管消失,微管排列无序,“9 2”变成“7 2”,继续后行,中央管和一个二联体微管消失,“7 2”变成“6”直至末段.这种微管终止模式是家猫精子的一个特征.     

HMBA对人成骨肉瘤MG-63细胞形态结构和终末分化指标表达的影响

应用环六亚甲基双乙酰胺处理人成骨肉瘤MG-63细胞,观察MG-63细胞处理前后形态与超微结构及其相关终末分化指标的表达变化.实验结果显示,经5 mmol/L HMBA处理后,MG-63细胞体积增大,趋于扁平铺展状态,细胞大小较为一致,排列较为规则,细胞核形态规则,核质比例减小,核仁减少,核内异染色质减少,常染色质增多,细胞核内的线粒体和高尔基体较为发达,内质网数量增多,细胞表面的微绒毛减少,在成熟细胞中可见钙化糖原颗粒,细胞表面出现钙化小泡沉积,并且形成典型的骨结节.常规细胞化学和免疫细胞化学检测显示,HMBA处理后的细胞中Ⅰ型胶原纤维、骨钙素、骨粘素的表达显著增加,实验结果表明HMBA能够有效诱导人成骨肉瘤细胞的分化,并促进其终末分化指标的表达.     

Cr~(6+)、As~(3+)污染对黑藻叶细胞伤害的超微结构研究

电镜观察发现 ,黑藻 (Hydrillaverticillata(L .F .)Royle)叶细胞遭受Cr6+ 、As3 + 毒害初期 ,高尔基体消失 ,内质网膨胀后解体 ,叶绿体中的类囊体和线粒体中的脊突膨胀 ,核中染色质凝集 .随着叶细胞毒害程度的加重 ,核仁消失 ,核膜破裂 ,叶绿体和线粒体解体 ,质壁分离使胞间连丝拉断 ,最后细胞死亡 .结果表明 :Cr6+ 、As3 + 对细胞的膜结构与非膜结构都产生毒害作用     

鸣禽前脑上纹状体腹侧尾核超微结构的观察

实验用鸟经脑灌流后于HVc处取材,用Epon 812包埋剂浸润包埋,经铀－铅电子染色后,H－600投射电镜下观察,IBAS图像分析集资进行处理,结果发现,鸣禽HVc核 的神经细胞以圆形,卵圆形为主,胞体体积无明显的种间差别,但雄鸟细胞比雄鸟大,HVc的突触连接以轴－树突触和轴－棘突触为常见,经统计学t检验HVc单位面积突触总数,I型和II型突触所占比较,棘突触所占比较的种间差异不显著（P＞0．05）,而性别差异显著（P＜0．05）,结果表明,鸣禽唯唱控制核团体积的性别差异不仅反映在显微水平,而且在亚显微水平亦表现出明显的性别二态性。     

纤毛虫阔口游仆虫(Euplotes eurystomus)微管类细胞骨架的超微结构

采用透射电镜术显示腹毛类纤毛虫阔口游仆虫(Euplotes eurystomus)的微管类细胞骨架包括纤毛器微管骨架和非纤毛器微管骨架.结果表明:细胞皮层纤毛器中除纤毛杆、纤毛基体微管外,其基体间连接纤维、基体托架、基体附属微管、纤毛器基部附属微管束以及围棘纤维篮等也是皮层纤毛器微管的重要组成部分,各种微管相互联系、聚...     

PCBs 对斑马鱼鳃及肝脏细胞超微结构的影响的初步研究

为了进一步了解ＰＣＢｓ对人类健康及生存的直接影响 ,作者对暴露在 50 μｇ/Ｌ质量浓度的ＰＣＢｓ水中的实验斑马鱼的鳃、肝脏细胞的超微结构进行观察研究 ,现将结果报道如下 .1　材料与方法实验斑马鱼 (Ｂｒａｃｈｙｄａｎｉｏｒｅｒｉｏ) ,体长 2 8- 3 5ｃｍ ,体质量 0 3 - 0 4ｇ,在水族箱暂养 1 0ｄ后活体取样 ,将实验组与对照组活鱼分别取其肝脏及鳃并用 4%戊二醛溶液和 1 %锇酸双固定 ,0 2ｍｏｌ磷酸缓冲液 (ｐＨ7 4)冲洗 ,各级乙醇脱水 ,环氧丙烷透明 ,Ｅｐｏｎ 81 2包埋 ,ＬＫＢ - 5型超薄切片机切片 ,醋酸铀及Ｒｅｙｎｏｌｄ…     

Ultrastructure of the prezoeal cuticle and integument of the first zoea of Pagurus brachiomastus (Thallwitz) (Anomura,Paguridae) just after hatching

Transverse sections of the prezoea stage of Pagurus brachiomastus (Thallwitz) were made to determine the fine structure of the prezoeal cuticle and the exoskeleton of the first zoea, by using transmission electron microscopy. The thin cuticle covering the newly hatched prezoea is 15–17 nm thick, which is approximately 24 times thinner than the zoeal cuticle. High resolution revealed a trilaminar structure of the prezoeal cuticle, which is composed of an outer layer, approximately 4·4 nm thick, a mid lighter layer, approximately 3·5 nm thick, and an inner layer, approximately 7·7 nm thick. During the prezoeal stage, the inner zoeal exoskeleton consists of an epicuticle and an exocuticle, measuring approximately 0·36 μm thick. At this stage, there were no indications of the formation of endocuticle.     

西北地区4种麻虻超微结构及其微量元素的初步观察

首次用于电子扫描显微镜对甘肃，宁夏和内蒙地区的甘肃麻虻Ｈ．ｋａｎｓｕｅｎｓｉｓ，等额麻虻Ｈ，ｈｅｄｉｎｉ，土麻虻Ｈ。ｔｕｒｋｅｓｔａｎｉｃａ，低额麻虻Ｈ．ｕｓｔｕｌａｔａ的触角，尾器外部开矿作了初步观察，并对尾器亚生殖板所含向量元素作了测析。     

低温胁迫下解放钟枇杷幼果细胞超微结构的变化

以解放钟枇杷幼果为试验材料,采用人工降温方法,研究低温胁迫下枇杷幼果超微结构的变化。结果表明：6℃、3℃低温胁迫时,解放钟果肉细胞质膜、液泡膜清晰,叶绿体、线粒体结构没有明显的变化;0℃低温处理条件下的原生质膜结构完整,少数叶绿体出现部分解体,双层膜有部分破裂现象,线粒体结构仍清晰可见;-3℃低温胁迫下,原生质膜、液胞膜均破裂,原生质体浓缩,叶绿体扭曲变形、相互融合,线粒体膜结构受损,脊消失。