大豆异黄酮增强大鼠骨密度作用的研究

大豆异黄酮(soybean isoflavone)是新型天然生理活性物质，具有多种生理功能．研究表明：连续饲喂大鼠90天，实验组股骨的长度、重量、骺端骨密度、中点骨密度均显著大于对照组，说明大豆异黄酮具有增强骨密度的作用．     

A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet

Summary A strong fibrinolytic activity was demonstrated in the vegetable cheese Natto, which is a typical soybean food eaten in Japan. The average activity was calculated at about 40 CU (plasmin units)/g wet weight. This novel fibrinolytic enzyme, named nattokinase, was easily extracted with saline. The mol. wt and pI were about 20,000 and 8.6, respectively. Nattokinase not only digested fibrin but also the plasmin substrate H-D-Val-Leu-Lys-pNA (S-2251), which was more sensitive to the enzyme than other substrates tried. Diisopropyl fluorophosphate and 2,2,2-trichloro-1-hydroxyethyl-o,o-dimethylphosphate strongly inhibited this fibrinolytic enzyme.     

钾对大豆产量及品质的影响

采用田间试验方法,研究了钾对大豆产量、产量构成因素及品质的影响。结果表明,钾对大豆产量构成因素有增强作用,钾能显著提高大豆产量,改善大豆品质。产量随着施K量的增加而增加,当施纯K量150kg/hm2时,高油品种东农434产量达2727kg/hm2,高蛋白品种黑农35产量达2651kg/hm2。施钾有提高大豆脂肪含量、降低蛋白质含量的趋势。表4,参6。     

氮磷钾配施对黑龙江西部半干旱地区大豆产量及效益的影响研究

在黑龙江省甘南县兴隆乡东兴村,进行了不同氮、磷、钾肥配比对大豆产量影响的研究。利用单因素分析法分析了氮、磷、钾对大豆产量的影响程度。并利用回归分析方法拟和了产量与氮、磷、钾肥之间的回归方程。图3,表5,参11。     

Detection of genetically modified ingredients in seed samples from Heilongjiang soybean field

A total of 210 soybean samples collected from different areas of Heilongjiang Province were analyzed by PCR to detect the presence of RR (Roundup Ready) soybean ingredient. Results showed that CaMV35S promoter was not detected in all samples. CP4-EPSPS gene was amplified in 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of PCR products. Further analysis by nested PCR indicated that there were no RR soybean ingredients in the samples. Besides, the amplified fragments of 0xl by CP4-EPSPS primers were sequenced, and the results confirmed again the fact that this fragment was not from CP4-EPSPS gene.     

富硒大豆低聚肽口服液的制备及其主要成分分析

富硒大豆蛋白经过酶解、超滤、调配、杀菌等工艺,制备成富硒大豆低聚肽口服液,分析了产品中的硒含量、大豆低聚肽含量及氨基酸组成,其中硒含量2.45 mg/100 ml,大豆低聚肽含量(分子量1000 D以下)达到638.25 mg/100 ml,含有Se-Cys在内的19种氨基酸,研究结果为富硒大豆低聚肽产品的质量标准制定提供了一定的理论依据.     

大豆抗营养因子对生长蛋鸡小肠内胰酶活性的影响

采用４８０只海兰褐蛋用雏鸡,以熟豆饼日粮为对照,从３周或９周开始给饲３个水平的生大豆日粮（４％,９％和１３％）,每期每个日粮４个重复,观察不同梯度大豆抗营养因子对不同生理阶段的生长蛋鸡小肠内胰蛋白酶活性的影响。在８,１３和１８周末每个重复随机抽取试鸡２只屠宰,观察小肠内容物胰蛋白酶活性的变化。结果表明,不同生理阶段给饲不同梯度大豆ＡＮＦｓ对８,１３和１８周龄雏鸡小肠内容物胰蛋白酶活性没有显著影响。     

soja亚属的数量分类学研究

以不同进化类型的7 个大豆居群为分类运算单位,选取了28 个形态学、生态学性状,对不同进化类型的大豆居群进行了数值分类研究.Q 分析及图论分析揭示野大豆、半野生大豆与栽培大豆之间存在较大差异.主成份分析表明前三主成份可保留总信息量的91 .05 % ,说明种内居群分类所选性状合理,并能在三维空间表达各居群间的相对位置.     

香菇酸豆奶发酵工艺的优化及营养成分分析

以香菇发酵滤液、豆浆和脱脂奶为原料,探讨了香菇酸豆奶的优化配方和发酵工艺.根据单因素实验,选择香菇发酵液添加量、豆浆添加比例和明胶添加量作为优化因素,以感官评分和持水力为响应值,设计响应面分析试验,确定合理的配方和生产工艺,并对产品的营养成分进行分析.结果表明,香菇酸豆奶的优化工艺参数:香菇发酵液添加量为20.15%(体积比),豆浆添加比例为3/11(体积比),脱脂奶添加比例为8/11(体积比),明胶添加量为0.17%(质量比),蔗糖添加量为6%(质量比),发酵剂添加量0.1%(质量比),42℃发酵5 h,产品酸甜爽口、营养丰富.香菇酸豆奶中的乳酸菌含量较高,持水力略高于对照酸豆奶,稳定性较好;产品中蛋白降解更充分,氨基酸含量较高,乙醛和丁二酮含量略高于对照组.