Acylphosphatase overexpression triggers SH-SY5Y differentiation towards neuronal phenotype

An acylphosphatase (AcPase) overexpression study was carried out on SH-SY5Y neuroblastoma cells, using a green fluorescent fusion protein (AcP-GFP), with GFP acting as a reporter protein. The cellular proliferation rate was significantly reduced by overexpression of AcPase by a factor of ten. In contrast, clones transfected with two inactive AcPase mutants showed a growth rate comparable to control cells. This suggests that AcPase catalyzes the proliferative down-regulation. AcPase-overexpressing clones showed a physiological mortality rate as assessed by an MTT reduction test and by evaluation of necrotic markers. DNA fragmentation analysis and assays of caspase-3 and poly (ADP-ribose) polymerase (PARP)-active fragments showed no evidence of any apoptotic pattern. AcPase overexpression led to a marked increase in PARP activity as well as Bcl-2 content; these are commonly up-regulated during differentiative processes in neuronal cells. In fact, the typical differentiation marker, growth-associated-protein 43, was significantly up-regulated. Microscopic observations also showed a clear increase in the differentiative phenotype in AcPase-overexpressing cells. Our results clearly show that AcPase plays a primary causative role in neuronal differentiation.Received 3 May 2004; accepted 25 May 2004     

复杂性与社会分化——卢曼社会系统理论研究

在卢曼看来,社会系统总是处于极端的复杂状态,同时也处于一种复杂化的过程之中。系统的复杂性征使其在面对环境的影响时,出现各种内在的危机和各种不平衡现象,并产生一系列的运作难题,使社会系统无法以其原来对付环境的原则进行实际运作,迫使社会系统不断地进行复杂性的化约和自我的区分化以化解危机,而社会系统也在此一过程中实现自身的演化和进化。     

Principle, traits and functions of electrochemical fingerprint of Chinese traditional medicine

In regard to such challenging analysis problems as scientific identification and quality evaluation of Chinese traditional medicine, the electrochemical fingerprint technique of Chinese traditional medi- cine has been researched. The principle, traits, functions, etc., of the electrochemical fingerprint have detailedly been analyzed and discussed by the B-Z oscillatory system using malonic acid as main dissipation substance, characterized by the fact that different influences of different Chinese traditional medicines on the mechanism of the oscillatory reaction cause different changes of the shape of the potential-time curve of the oscillatory system under the conditions of constant tem- perature and pressure. An economical, simple and convenient, easy pushing and effective scientific method for distinguishing and evaluating multifarious Chinese traditional medicines has success- fully been put forward.     

变化及复杂织物组织的计算机生成

采用织物组织的数学函数组织异化法,对三原变化组织进行计算机模拟,并在此基础上,对复杂组织的处理方法进行探索,旨在建立一种高效简单的织物组织的计算机生成方法,最后给出计算机模拟效果图.     

Influence of long-period pesticide force on genetic polymorphism of wolf spider Pardosa pseudoannulata (Lycosidae: Araneae)

Wolf spiders are predators in large quantities in the fields. How wolf spiders keep their dominant species status under long-period pesticide force? To answer this question, eight geographical populations of Pardosa pseudoannulata were used as materials to test the influence of geographical habitats on their genomic DNA polymorphism. The RAPD pattern showed polymorphic variations among and within different populations. Total 84 bands amplified by 10 random primers, of which 62 (73.81%) are polymorphic, were generated from 55 individuals of eight geographical populations. Meanwhile, Shannon’s index (Ho= 0.5177) showed a rich genetic diversity of P. pseudoannulata, and most of the genetic variation (64.24%) was found within populations. Multiple regression analysis suggested that it is the climatic variation (such as annual average temperature etc.) that results in adaptive eco-geographic differentiation, and it is the long-period pesticide force that speeds up the genetic differentiation of P. pseudoannulata which changed the genetic diversity of the population.     

用博弈论分析摩托罗拉网络服务的策略

基于豪泰林的地点差异化模型的分析研究,以摩托罗拉网络服务为例,分析了摩托罗拉作为跨国大公司在面对市场中采用低价策略的国内小电信网络服务公司的竞争时,采取差异化策略推出一系列的新型移动网络服务产品,从而获得竞争优势的内在因素和实施方法.从而进一步表明:为避免价格竞争,获取市场份额和利润,差异化是竞争战略的重要手段;作为国际性大企业,类似摩托罗拉公司,有能力、有条件实施差异化,提供差异化服务,并最终在绩效上有了良好的表现;差异化和消费者的赶路成本、偏好紧密相关,产品差异化要满足不同用户不同地点的不同需求.     

改革开放以来中国区域经济分异研究综述与展望

本文从阐述区域经济分异的含义入手,综述了国外对区域经济分异的研究和对中国区域经济分异的研究,进而提出了未来中国区域经济分异研究的热点应主要集中在地级行政区之间的区域经济分异。     

Determining method and conditional factors of electrochemical fingerprint of Chinese traditional medicine

The thermodynamic systems and kinetic model suitable for the electrochemical token of the compo-nent throng in Chinese traditional medicines have been analyzed. It has been indicated that the damp oscillatory reactions in the non-equilibrium close system and open system without the supplement of dissipative substance have important significance for the throng token and analysis of chemical com-ponents in Chinese traditional medicines. Various factors influencing the electrochemical fingerprint,such as reactant species and their concentrations,electrode types,temperature,stir rate,the kind,dosage and granularity of Chinese traditional medicines,have amply been researched by the B-Z os-cillatory reaction which used malonic acid as a main dissipative substance. In addition,the quantitative information about the chemical components in Chinese traditional medicines has been discussed in detail. The method and its conditions for determining the electrochemical fingerprint used in scientifi-cally distinguishing and evaluating Chinese traditional medicines have successfully been put forward.     

对几道有机化学反应式写法的辨析

依据化学反应历程对几种不同教科书中的烯烃与卤素加成、氢化铝锂或硼氢化钠还原醛酮、卤仿反应的相关习题进行科学的辨析，对如何得出准确、规范和严谨的答案进行探讨。     

导数在函数图象对称性判断中的应用

利用导数的一些性质,发现了函数图象的对称性与函数的一阶、二阶导数的密切关系．根据这些关系,找到了一种判定函数图象是否关于某一直线对称或关于某点成中心对称的方法,这种方法是导数在研究初等函数中的又一应用,用它可以方便地讨论函数的对称性,有较广泛的应用价值．