文蛤(Meretrix Meretrix)凝集素(MML)的初步研究——Ⅰ.MML在文蛤体内的分布及纯化

在文蛤(Meretrix Meretrix)体内具有血球凝集活性的凝集素(简称MML),经实验证实仅分布于文蛤外套腔液中。向外套腔液中加入固体硫酸铵达40％时,可使99％的MML沉淀。用不同饱和度的硫酸铵溶液依次对上述沉淀进行抽提,得到MML粗制品溶液,其比活比原始外套腔液提高了约9.95倍,总活力回收达48％。最后用自制的人血浆糖蛋白一Sepharose 4 B亲合层析柱可将上述MML粗制品的比活再提高400多倍。结果与原始外套腔液比较,MML终产品的比活总共提高了4313倍,总活力回收达44.8％,在电泳鉴定时显示出单一的区带。     

龙门山地区泥盆纪锶、碳同位素与海平面变化

文章将龙门山地区泥盆系划分为四个沉积旋回,即四个地层层序,建立了该区泥盆纪海相碳酸盐锶、碳同位素地层曲线,并将同位素演化与沉积旋回结合起来研究该区泥盆纪的海平面变化。     

降低砼人工骨料开采大块率爆破试验研究

从理论上提出了降低隔河岩水利枢纽砼人工骨料开采大块率的小抵抗 线深孔微差爆破技术．通过现场试验确定了合理的钻爆参数和微差起爆方 式，给出了相应的技术措施．     

低中碳合金结构钢深冷处理试验研究

本文对25MnV、40C_r等合金结构钢进行深冷处理,深冷处理后冲击韧性均得到不同程度的提高。回火温度越低、冲击韧性值提高幅度越大,表明钢的基体组织过饱和度是影响深冷处理强韧比效果的重要因素。     

莱州湾东南沿岸海水入侵发展规律与趋势预测

对莱州湾东南沿岸地区海水入侵的特点和规律进行了分析，并以灰色系统模型ＧＭ对该地区海水入侵的发展趋势进行了预测，最后提出了防治海水入侵的对策建议。     

窄幅深耕犁翻土覆盖性能的初探

通过“埋块试验”探讨了犁体宽深比和耕深对翻土覆盖性能的影响,证明了窄幅深耕犁的翻土覆盖性能能满足农艺要求。发现犁的宽深比还影响土垡在行驶方向上的位移距离,用窄幅深耕犁时土垡在行驶方向上的位移距离明显小于普通铧式犁,这很可能是窄幅深耕犁比阻降低的主要因素之一。     

水压支柱支护特性及其在深井开采中的应用

研究了水压支柱的工作原理、支柱的阻力特性曲线以及水压支柱的等效刚度和阻力增量等参数,并确定了工作面顶板下沉量与支柱初撑力、支柱阻力增量之间的相互关系,求出了水压支柱的最小初撑力、额定初撑力和额定支柱工作阻力等参数的计算方法;此外,还对水压支柱的让压性能及其在湘西金矿深井缓倾斜复杂薄矿脉开采工艺技术中的应用进行了分析.得出:湘西金矿深部试验采区水压支柱的额定初撑力为162kN,额定工作阻力为200kN,合理支护网度为2.0m×2.0m;水压支柱在湘西金矿的使用,改善了采空区顶板的受力条件,为落矿、运搬及充填工作提供了良好的作业环境,为缓倾斜薄矿脉强化开采提供了可靠的安全保障.     

Notable diversity in hemoglobin expression patterns among species of the deep-sea clam, <Emphasis Type="Italic">Calyptogena</Emphasis>

The deep-sea clams Calyptogena nautilei and C. tsubasa, which live in the cold-seep area at a depth of 3570 m in the Nankai Trough, Japan, have abundant hemoglobins (Hbs) in erythrocytes, similar to other Calyptogena species. We determined the cDNA-derived amino acid sequences of Hbs from two Calyptogena species. C. tsubasa was found to contain two dimeric Hbs, Hb I consisting of 145 amino acid residues and Hb II with 137 residues, similar to known Hbs from C. soyoae and C. kaikoi. Sequence identity was over 90% among the orthologous chains of Calyptogena Hbs. On the other hand, surprisingly, C. nautilei contained two monomeric Hbs, Hb III containing 141 residues and Hb IV with 134 residues. In addition, Hbs III and IV showed only 33–42% sequence identity with Hbs I and II from other Calyptogena species. The distal (E7) histidine, one of the functionally important residues of the heme protein, is replaced by glutamine in all Hb chains of Calyptogena species. A phylogenetic analysis indicated that C. nautilei Hb III is closer to Hb I from other Calyptogena species. We suppose that a Hb gene was duplicated at least three times in an immediate ancestor of Calyptogena and, presumably depending on physiological conditions different Hb sets are being expressed: dimeric Hbs I and II in C. soyoae, C. kaikoi and C. tsubasa, and monomeric Hbs III and IV in C. nautilei. Received 13 May 2003; received after revision 5 June 2003; accepted 12 June 2003     

A weak El Nio event in 2002 detected by the astronomical observation

Using the length-of-day (LOD) data series derived from astronomical observations made by various modern space techniques, southern oscillation index (SOI) and anomalies of sea surface temperature (SST) in tropical Pacific Ocean, the inter-annual components of these data series are obtained by the technique of band filtering from January 1962 to October 2002. It is found that a new ENSO event has already occurred in 2002 and been detected by the interannual LOD. It is shown by the wavelet analysis that the component with a period of about 4-6 years and the quasi-biennial component are out of phase during 2001-2002, which consequently suggests that the new ENSO event cannot be a strong one.