Enzyme-Mediated Hydrolysis of Poly(ethylene glycol)-Supported Carbonates

1Introduction Enzymatic kinetic resolution of racemic alcohols or esters is known as a useful method for the preparation of optically active secondary alcohols. However, the work-up including the separation of the mixture of the remaining substrate and the resulting compound spend a lot of time and waste much amount of solvents. On the other hand, organic synthesis based on polymer supports has made rapid progress. Although the methodology is potentially useful for the easy separation of compounds obtained by the enzymatic reaction, there have been relatively few reports on enzymatic resolutions of using a polymer so far. We have noticed that using a watersoluble polymer could be suitable for enzymatic transformation. Here, we report the first example of an enzyme-mediated enantioselective hydrolysis of poly(ethylene glycol)(PEG)-supported substrates with a carbonate moiety to afford optically active compounds, and the method enables us to achieve the easy separation of the products[1]. See Scheme 1.     

水溶性三聚氰胺甲醛树脂的合成及其性质的研究

研究了固体含量大于50％,粘度100秒(B-4杯,25℃),有效使用期6个月以上的三聚氰胺甲醛树脂的合成方法。测定结果表明,该样品与美国ACCOBOND 3900锚固剂比,IR吸收峰基本相同,分子量为247(占78％)、492(占16％)和1.5×10~4(占6％)。该树脂用作防潮玻璃纸的锚固剂,效果很好,热封值为93g/cm我们还合成了三聚氰胺甲醛粉状树脂,可直接用于嘧胺塑料制品的表面罩光,增加光泽度和耐磨性。     

人参水溶性总蛋白对小鼠黑色素瘤细胞B16的增殖抑制的研究及对Bcl-2/Bax表达的影响

摘 要 目的：研究人参水溶性总蛋白对小鼠黑色素瘤细胞的增殖抑制作用及对Bcl-2/Bax表达的的影响。方法：1.MTT法测定人参水溶性总蛋白对黑色素瘤B16细胞株的增殖抑制率。2. RT-PCR（Real-time PCR）法检测Bcl-2、Bax基因的mRNA表达情况。3.Western blot测定凋亡蛋白Bcl-2和Bax的表达。结果：1.人参水溶性总蛋白对小鼠黑色素瘤B16细胞株有明显的增殖抑制作用,其抑制率随着加药浓度的增加而升高,并与加药浓度呈现一定的量效关系。2.随着人参水溶性总蛋白浓度的增高（0,100,500,1000ug/mL）,RT-PCR检测的Bcl-2 mRNA相对表达量逐渐降低,Bax mRNA相对表达量逐渐升高,当加药浓度为1000ug/ml时,Bcl-2的相对表达量最低,为0.20±0.05,Bax的相对表达量最高,为16.83±0.07;与对照组相比,Bcl-2和Bax基因的相对表达量差异均有统计学意义（P＜0.05）。3.经Western blot测定,各组Bcl-2蛋白的表达均低于对照组,各组Bax蛋白表达均高于对照组,且随着加药浓度的增加,Bcl-2蛋白表达量逐渐降低,Bax蛋白表达量逐渐升高。结论：人参水溶性总蛋白可以抑制小鼠黑色素瘤B16细胞株增殖,其分子机制可能与调控Bcl-2/Bax凋亡蛋白的表达有关。     

2-（2-甲氧基-4-硝苯基）-3-（4-硝苯基）-5-（2,4-二磺基苯）-2H-四唑单钠盐的合成方法改进

2-（2-甲氧基-4-硝苯基）-3-（4-硝苯基）-5-（2,4-二磺基苯）-2H-四唑单钠盐（6）是用于细胞增殖和细胞毒性试验的CCK-8试剂盒的主要成分,该试剂盒具有操作方便、重复性好,灵敏度高等优点.改进了化合物（6）合成方法,使用N-溴代丁二酰亚胺（NBS）氧化甲臜（5）中间体,路线总收率34.4%.同时,利用高分辨质谱（HRMS）、核磁共振（NMR）、熔点测定、紫外光谱（UV）方法对目标产物（6）进行了结构验证.实验方法具有路线简单、操作安全、原料价廉易得等优点,并且后处理简单,适合工业生产.     

水溶性硫化黑BR染色性能和检验办法

介绍了水溶性硫化黑BR的标准技术指标，从强度和色光检测、分光强度测定等方面阐述了水溶性硫化黑BR染色性能和检验方法。     

Water-soluble chitosan nanoparticles as a novel carrier system for protein delivery

High MW chitosan (CS) solutions have already been proposed as vehicles for protein delivery. The aim of the present work is to investigate the potential utility of water-soluble chitosan (WSC) as vehicles to load and deliver proteins. WSC nanoparticles (WSC NP) with various formations were prepared based on ionic gelation of WSC with pentasodium tripolyphosphate (TPP) anions. Bovine serum albumin (BSA) was used as a model protein drug incorporated into the WSC nanoparticles. Blank and BSA-loaded WSC nanoparticles were examined and determined to have a spherical shape with diameters between 35―190 nm, and zeta potential between 35―42 mV. FTIR confirmed that the tripolyphosphoric groups of TPP linked to the ammonium groups of WSC in the nanoparticles. Some factors affecting delivery properties of BSA have been investigated. Altering the concentration of BSA from 0.05 to 1 mg/mL enhanced the loading capacity of BSA but decreased loading efficiency simultaneously. Also, with the introduction of poly ethylene glycol (PEG), BSA release accelerated. Nanoparticle preparation from WSC with various deacetylation degrees (DDs) from 72.6% to 90% and MWs ranging from 3.5 to 15.8 kDa promoted loading efficiency and decreased the release rate. These results indicate that WSC nanoparticles are promising carriers for protein delivery.     

元坝地区雷口坡组气源与碳同位素倒转成因

探讨四川盆地元坝地区雷口坡组气源与碳同位素倒转成因.通过雷口坡组烃源岩与天然气地化参数分析,其烃源岩以泥晶灰岩、含泥灰岩为主,TOC质量分数平均为0.37％,生烃能力较差.天然气中非烃气的体积分数多数＜10％,烷烃气组分较干,CH4相对体积分数平均为99.0％,碳同位素较轻,其中δ13C1均值为-34.19‰,δ13 C2均值为-33.31‰,且多数样品发生δ13C1,δ13C2倒转;甲烷氧同位素较轻,均值为-156.3‰.与相邻层位烷烃气对比并结合烃源背景,综合分析认为其气源主要为须家河组媒型气,其甲烷、乙烷碳同位素倒转为水溶分馏作用导致气层中乙烷变轻程度超过甲烷一定幅度引起.     

魔芋中水溶性多糖的提取

探讨了对不同的料液比、提取次数、提取时间、提取温度等对魔芋中水溶性多糖提取的影响，结果表明，魔芋中水溶性多糖的提取的最适宜条件是料液比为1：100，提取温度为90℃，提取时间为2h，提取次数为3次．     

莺歌海盆地水溶相天然气析出成藏特征

通过莺歌海盆地的烃源岩、温压特征及底辟活动规律研究,对水溶气出溶成藏的条件进行分析,并根据天然气的地球化学和同位素特征,研究水溶气出溶成藏的证据。结果表明:莺歌海盆地烃源岩气源供给充足,中浅层和深层天然气溶解度差异大,底辟构造周期性开启提供了流体向上运移的通道,具备水溶气运移出溶成藏的理想条件;在中浅层1.5~2.0 km深度范围内,天然气干燥系数较大、异构烷烃与正构烷烃含量比值大、甲烷碳同位素组成偏重、芳烃富集,表明水溶气出溶成藏是中浅层气藏形成的重要机制之一;在底辟活动期间,深层溶解大量天然气的水沿着底辟形成的断裂运移至中浅层,由于温压大幅度降低,天然气从水中释放,进入到底辟上部或附近的圈闭中形成气藏。     

郑州市PM2.5和PM10中水溶性无机离子的污染特征

于2009年10月至2010年8月间采集郑州市大气颗粒物PM2.5与PM10样品,对其质量浓度及水溶性离子进行分析研究.结果表明：PM2.5在秋、冬、春、夏四季的质量浓度的平均值分别为134.9、121.6、77.9和102.0μg/m^3,PM10在秋、冬、春、夏四季的质量浓度的平均值分别为193.2、184.0、140.9和140.5μg/m^3,日均值超标率分别达77.8%和59%.PM2.5和PM10质量浓度呈现很好的相关性,春季粗粒子在PM10中的比例相对较高,而秋、冬和夏季细粒子是PM10的主要组成部分.主要的水溶性离子是SO4^2-、NO3^-和NH4^＋,大部分以（NH4）2SO4和NH4NO3形式存在;NO3^-和SO4^2-质量比小于1,说明采样期间郑州市大气以固定排放源污染为主.