长春地铁某区间降水方案设计与优化

以长春地铁某区间降水工程为例，介绍了基坑涌水量、单井出水量及降水井井深、数量和间距的计算，并用有限元软件Midas GTS NX 对不同的降水井井间距进行模拟分析，得到了适宜的降水井井间距和井水位降深值，达到了既能满足现场施工的要求又能节约降水工程造价的目的。     

渤中34-1N钻井帽在锦州9-3油田井口平台上的应用

渤中34-1N钻井帽始建于2009年,计划在渤中34-1N油田利用其与非悬臂式钻井船(渤海5号)在导管架上方进行钻完井作业。但在钻井帽建造后期,悬臂式钻井船的紧张局面得到缓解,钻井帽被放置于建造场地4年。鉴于锦州9-3油田综合调整项目新建1座四腿井口平台,初步考虑利用钻井帽在锦州9-3油田井口平台的导管架上进行钻完井作业。因此,需要对钻井帽进行适应性改造。     

日食对地球元素谱线波长的影响

1987年9月23日日食期,日食观测组拍摄了地球元素H,D,Ca,Fe,…的发射光谱(光源和仪器安置在同一实验室内),首次发现在日食期地球元素谱线波长发生变化,H,D,Ca谱线波长的相对变化达10~(-4)量级。     

用剪切干涉条纹测双棱镜的折射棱角

用激光楔形板剪切干涉,测量干涉条纹的间距,得出了双棱镜的折射棱角。对测量结果进行了讨论,并与劈尖法结果进行比较。     

杨粮复合系统内生态因子的变化及对小麦产量的影响

对徐淮平原杨粮复合系统内不同物候期生态因子的变化规律进行了研究。结果表明：在杨树展叶期,不同株行距生态因子变化不明显,能满足小麦生长需要;在杨树展叶后的全叶期到盛叶期,不同株行距生态因子变化有明显差异,并对小麦生长有一定影响。从实验区不同复合模式下小麦产量的高低考虑该区最佳的复合模式为：杨树间作行距≥30m,林网200m×300m.     

高位钻孔瓦斯抽放参数的确定

为防治煤矿瓦斯灾害和有效开采瓦斯资源,基于高位钻孔瓦斯抽放理论,在分析采空区顶板覆岩空间垮落规律及采动裂隙"0"形圈理论基础上,给出了钻孔有效高度范围的理论计算方法及钻孔沿倾向的布置范围,得出了钻场间距应小于实际施工的钻孔长度与钻孔重叠区之差的结论.在现场应用中取得了良好的技术与经济效果.     

刮板输送机刮板间距的优化

分析了刮板推移物料运动过程的受力状态,为保证物料稳定运行,以输送机运行阻力最小为前提,推导出刮板最大间距计算式,分析了影响因素;研究了回空侧溜槽箱内充满物料的极端工况下,链条运行阻力产生机理,推导出运行阻力的计算式,以输送机运行阻力最小为目标,优化刮板间距;实例分析证明,目前工程上使用的刮板输送机在近水平布置时,刮板间距在1m时是比较合理的.     

Alpha-crystallin: an ATP-independent complete molecular chaperone toward sorbitol dehydrogenase

-Crystallin, the major component of the vertebrate lens, is known to interact with proteins undergoing denaturation and to protect them from aggregation phenomena. Bovine lens sorbitol dehydrogenase (SDH) was previously shown to be completely protected by -crystallin from thermally induced aggregation and inactivation. Here we report that -crystallin, in the presence of the SDH pyridine cofactor NAD(H), can exert a remarkable chaperone action by favoring the recovery of the enzyme activity from chemically denaturated SDH up to 77%. Indeed, even in the absence of the cofactor, -crystallin present at a ratio with SDH of 20:1 (w:w) allows a recovery of 35% of the enzyme activity. The effect of ATP in enhancing -crystallin-promoted SDH renaturation appears to be both nonspecific and to not involve hydrolysis phenomena, thus confirming that the chaperone action of -crystallin is not dependent on ATP as energy donor.Received 28 October 2004; received after revision 22 December 2004; accepted 10 January 2005     

Hydra vulgaris ω10-lipoxygenase is used in vivo to synthesize new α-linolenic acid metabolites

Previous studies conducted in cytosolic extracts of the freshwater hydrozoanHydra vulgaris led to the finding of an abundant 11(R)-lipoxygenase catalyzing the peroxidation of polyunsaturated fatty acid (PUFAs) on the tenth carbon atom from the aliphatic end (10 peroxidation). Here we describe experiments aimed at identifying the actual metabolites generated in vivo by such enzymic activity. Homogenates ofH. vulgaris polyps were analyzed by HPLC. This showed the presence of three major components chromatographically identical to three metabolites obtained when incubating the homogenates with exogenous -linolenic acid (-LA). The presence, in extracts of polyps prelabelled with [¹⁴C]--linolenic acid, of radioactive metabolites displaying the same chromatographic properties, substantiated the hypothesis that the natural products isolated in vivo are derived from -LA. Gas chromatographic analyses revealed that this was the most abundant PUFA in both free and phosphoglyceride-bound fatty acid pools. [¹H]-NMR analysis of the endogenous substances, carried out in comparison with products obtained from exogenously incubated -LA, indicated that their structures were those of 9-hydroxy-, 9-hydroperoxy- and 9-keto-octadeca-10E-12Z-15Z-trienoic acids (9--HOTrE,-HPOTrE and-KOTrE).Hydra homogenates transformed 9--HPOTrE partly into 9--HOTrE and partly into 9--KOTrE. Chiral phase HPLC conducted on 9--HOTrE established that this metabolite was composed mostly of theR anantiomer. These observations, and the finding that the presence of exogenous arachidonic acid in incubated homogenates significantly reduces the production of -LA metabolites, provide strong evidence that these compounds are produced by an enzymic activity identical to the previously-describedH. vulgaris (R)-10-lipoxygenase. Further experiments suggested that -LA, acting as the native substrate for this enzyme, is mainly esterified on the 2 position ofHydra phosphoglycerides, and that the production of the -LA metabolites described here for the first time from natural sources, can be potentially enhanced in vivo by stimuli activating phospholipase A₂.