维生素K3对安定血药浓度及药理作用的影响

用动物实验研究了维生素Ｋ３对安定血药浓度及药理作用的影响。结果表明，维生素Ｋ３能明显缩短安定血药浓度分布半衰期和消除半衰期，提高安定体内清除率。增加家兔呼吸频率，但不影响麻醉后家兔的心电图、血压和心率。能缩短安定所致小鼠睡眠时间，具有催醒作用。     

磁性聚乙二醇微球固定化辅酶维生素B6的研究

以制备的磁性聚乙二醇胶体粒子为载体，戊二醛为交联剂，固定化辅酶维生素B6，对固定化的条件进行了探索，确定了固定化的最佳条件：室温下，0.03g磁性聚乙二醇微球与5mL(8%)戊二醛交联，固定0.01g辅助酶维生素B6；振荡时间为12小时。     

生物合成麦角甾醇酵母菌株选育

采用24株不同种属的酵母菌进行发酵培养，研究其麦角甾醇的生物合成，并筛选Yeg一14菌株为实验菌。以玉米粉水解液为碳源，尿素(NH2)2CO为氮源，通过两步深层发酵工艺路线，研制麦角甾醇酵母。     

柿树叶Vc含量的对比性研究

本文对各个时期、各个季节的柿树叶维生素C含量进行了大量 的定量分析和对比性研究，同时严密而完整地说明其生理作用，旨在更好地认识柿树叶的经济价值，使其得以有效的开发和利用。     

广东8种野菜中硝酸盐、亚硝酸盐及Vc的含量

测定了广东菊科8种野菜中硝酸盐、亚硝酸盐和维生素c的含量．结果表明：革命菜、一点红、苣荬菜的硝酸盐含量低于轻度污染水平，属于一级野菜；山莴苣、地胆草、加拿大蓬和艾的硝酸盐含量低于785mg/kg，达中度污染水平，属二级野菜范围，不宜生食，煮熟或盐渍可安全食用；甜菜籽属于三级野菜，不可生食和盐渍，可熟食,这些野菜维生素C的含量均低于50mg／100g 500mg/kg(鲜重)，属于中、低维生素C含量的野菜。     

维生素K3对Cu(Ⅱ)-H2O2-NaSCN化学振荡反应的影响

研究了在碱性条件下,维生素K3(以下简称VitK3)对Cu2 催化H2O2和NaSCN的化学振荡反应(以下简称铜体系)的影响.结果表明,VitK3的加入会使振荡图形的周期和振幅均增大,且VitK3浓度与加入VitK3后第一周期的改变值(ΔT1)呈良好的线性关系.线性范围分别为5 26×10-7～2 17×10-4mol/L和3 21×10-4～8 87×10-3mol/L,相关系数分别为0 9981和0 9975.初步探讨了VitK3对铜体系振荡反应影响的机理.     

高效液相色谱法测定保健食品中维生素B12的含量

采用高效液相色谱法测定保健食品中维生素B12含量,色谱柱用Agilent ZORBAX SB-Aq (4.6mm×250mm,5μm), 流动相为甲醇-0.2%磷酸水溶液(38:62),流速1.0ml/min,检测波长361nm.结果是维生素B12线性范围为0.816～4.08μg,r=0.9997;平均回收率为97.2%,RSD为1.39%(n=6),最小检出限为0.85μg/g.高效液相色谱法灵敏度高,操作简便,重现性好,结果准确,可以用于保健食品中维生素B12的质量控制.     

Inhibition of lung carcinoma cell growth by high density lipoprotein-associated α-tocopheryl-succinate

-Tocopheryl-succinate (TS) is a synthetic, anti-neoplastic derivative of -tocopherol. Here we studied the effects of free and high-density lipoprotein subclass 3 (HDL₃)-associated TS on the growth of human (A549) and mouse Lewis (LL2) lung carcinoma cells. Both free and HDL3-associated TS inhibited A549 growth in a time- and concentration-dependent manner. Treatment of A549 cells with TS-enriched HDL3 led to DNA fragmentation and a time-dependent decrease in immunoreactivity of poly(ADP-ribose)polymerase. Uptake experiments revealed a high capacity for selective TS uptake in excess of holoparticle endocytosis. Overexpression of scavenger receptor class B, type I (SR-BI), the prime receptor mediating selective lipid uptake, in A549 cells resulted in significantly increased selective TS uptake, a finding associated with complete cellular growth arrest. The present in vitro findings were verified in an in vivo model: tumor inoculation in C57BL6 was performed with either wild-type, -galactosidase- or SR-BI-overexpressing LL2 cells. After tumor inoculation, the animals received six consecutive intravenous injections of TS. This experimental setup resulted in significantly reduced tumor burden in animals that were inoculated with SR-BI-overexpressing LL2 cells but not in animals inoculated with wild-type or -galactocidase-transfected cells. Based on our in vitro and in vivo findings, we propose that SR-BI could provide a novel route for HDL₃-mediated drug delivery of anti-neoplastic drugs.Received 8 March 2004; received after revision 7 April 2004; accepted 26 April 2004     

纳米金光度法测定维生素B1

采用柠檬酸钠还原氯金酸的方法制得粒径约为13 nm的纳米金.在酸性环境中纳米金与维生素B1发生相互作用,纳米金产生凝聚,使其最大吸收峰发生红移,由此建立维生素B1的比色测定方法.优化了溶液pH、纳米金的浓度以及反应时间.在最佳条件下,方法的线性范围为5～ 60 ng/mL,检测限为0.74 ng/mL.用此方法成功分析了维生素B1片剂和注射液中维生素B1的含量.